Inflammageing assessed by MMP9 in normal Japanese individuals and the patients with Werner syndrome

Goto, Makoto; Chiba, Junji; Matsuura, M.; Iwaki-Egawa, Sachiko; Watanabe, Yasuhiro

doi:10.5582/irdr.2016.01028

Cited by 5 publications

(6 citation statements)

References 24 publications

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“…However, these relationships would need to be confirmed in a more extensive study of a larger cohort of healthy non‐smokers and smokers. In healthy adults, serum NGAL is correlated with age , but serum MMP‐9 may not be . In the present study, age‐corrected serum levels of MMP‐9 and proMMP‐9/NGAL were correlated with smoking status in patients with COPD but not in healthy smokers.…”

Section: Discussioncontrasting

confidence: 46%

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Concomitant elevations of MMP‐9, NGAL, proMMP‐9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease

Bchir

Nasr

Bouchet

et al. 2016

J Cellular Molecular Medi

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A growing body of evidence points towards smoking‐related phenotypic differences in chronic obstructive pulmonary disease (COPD). As COPD is associated with systemic inflammation, we determined whether smoking status is related to serum levels of matrix metalloproteinase‐9 (pro‐ and active MMP‐9), neutrophil gelatinase‐associated lipocalin (NGAL) and the proMMP‐9/NGAL complex in patients with COPD. Serum samples were collected in 100 stable‐phase COPD patients (82 smokers, 18 never‐smokers) and 28 healthy adults (21 smokers, 7 never‐smokers). Serum levels of studied factors were measured in ELISA. Our data provide the first evidence of simultaneously elevated serum levels of MMP‐9, NGAL and proMMP‐9/NGAL in COPD smokers. While the triad discriminated between smokers and non‐smokers in the COPD group, MMP‐9 and proMMP‐9/NGAL (but not NGAL) discriminated between smokers with and without COPD. Adjustment for age and smoking pack‐years did not alter the findings. Serum MMP‐9, NGAL and proMMP‐9/NGAL levels were not correlated with the GOLD stage or FEV1 decline. Furthermore, serum levels of neutrophil elastase (NE) and MMP‐3 (but not of IL‐6 and MMP‐12) were also higher in COPD smokers than in healthy smokers before and after adjustment for age and pack‐years. Among COPD smokers, levels of MMP‐9, NGAL and proMMP‐9/NGAL were positively correlated with NE (P < 0.0001) but not with the remaining factors. Gelatin zymography detected proMMP‐9 in serum samples of healthy and COPD smoking groups. Our results suggest that associated serum levels of proMMP‐9, NGAL, proMMP‐9/NGAL and NE may reflect the state of systemic inflammation in COPD related to cigarette smoking.

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Section: Discussioncontrasting

confidence: 46%

“…However, these relationships would need to be confirmed in a more extensive study of a larger cohort of healthy non-smokers and smokers. In healthy adults, serum NGAL is correlated with age [58,59], but serum MMP-9 may not be [60][61][62].…”

Section: Discussionmentioning

confidence: 99%

Concomitant elevations of MMP‐9, NGAL, proMMP‐9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease

Bchir

Nasr

Bouchet

et al. 2016

J Cellular Molecular Medi

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“…We have already reported the increasing level of serum hsCRP and MMP-9 in accordance with healthy calendar aging and also significantly more elevation of hsCRP compared with healthy aging controls in patients with a genetically-determined progeroid syndrome such as WS (4,5).…”

Section: Discussionmentioning

confidence: 67%

“…Human aging is inevitably encountered by an increasing chance of environmental attack from infectious agents such as herpes viruses during daily living leading to a minor/latent inflammation that could be evaluated by highly sensitive CRP (hsCRP) and matrix metallo-proteinase-9 (MMP-9) ( 1,2,4,5).…”

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confidence: 99%

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Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome

Goto

Chiba

Matsuura

et al. 2018

IRDR

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Human aging is probably controlled by a combination of intrinsic/genetic factors including metabolism/ catabolism functions (1), and extrinsic/environmental factors such as infections (2). The possible intrinsic/ genetic factors include genetically-determined progeroid syndrome genes such as WRN for Werner syndrome (WS) (3). WS has been proposed as the representative human natural ageing model. Because patients with WS, transmitted autosomal-recessively by the homozygous mutation of WRN (RecQ3 DNA helicase), usually manifest their premature aging phenotypes immediately after adulthood. Typical aging signs and symptoms in WS include voice change, bilateral cataracts, gray hair/alopecia, skin atrophy, skin pigmentation, type II diabetes mellitus, central obesity, atherosclerosis, hyperlipidemia, skin ulcer, renal dysfunction, osteoporosis, and cancer/sarcoma. The Summary A series of our "inflammageing" study examining serum samples from a maximum of 217 healthy Japanese individuals aged between 1 and 100 years and mutation-proven 40 patients with Werner syndrome (WS) indicated normal aging-associated elevations of highly sensitive CRP (hsCRP) and matrix metalloproteinase-9 (MMP-9). To further study the contribution of environmental factors such as persistent herpes viral infection to inflammageing, IgG antibodies against varicella/zoster virus (VZV) and cytomegalovirus (CMV) were examined in the same serum samples as has been done for hsCRP and MMP-9 analyses. The mean levels of serum IgG viral antibodies were comparable between normal (mean ± SE: 31.0 ± 4.3 unit) and WS (38.6 ± 7.6) for CMV, and between normal (42.0 ± 12.2) and WS (29.8 ± 3.8) for VZV, respectively. Significant associations of aging with IgG anti-CMV antibody were in normal aging (p = 0.023) and WS (p = 0.037), but not with IgG VZV in both conditions. Aging-associated change of IgG anti-CMV antibody titer in WS increased significantly (1.32 times higher) compared with normal aging (p = 0.037). IgG anti-CMV level was significantly elevated in the male gender than female in both conditions (p = 0.006). Elevated hsCRP level was significantly associated with IgG anti-CMV (p = 0.016) and IgG anti-VZV (p = 0.008) antibodies in normal aging, but not in WS. Serum MMP-9 was significantly associated with IgG anti-CMV level (p = 0.0002) in normal aging, but not in WS. Persistent herpes viral infection may constitute a part of "inflammageing" in normal aging and WS.

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Nonfunctional mutant Wrn protein leads to neurological deficits, neuronal stress, microglial alteration, and immune imbalance in a mouse model of Werner syndrome

Hui

St‐Pierre

Detuncq

et al. 2018

Brain, Behavior, and Immunity

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Inflammageing assessed by MMP9 in normal Japanese individuals and the patients with Werner syndrome

Cited by 5 publications

References 24 publications

Concomitant elevations of MMP‐9, NGAL, proMMP‐9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease

Concomitant elevations of MMP‐9, NGAL, proMMP‐9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease

Aging-associated latent herpes viral infection in normal Japanese individuals and patients with Werner syndrome

Nonfunctional mutant Wrn protein leads to neurological deficits, neuronal stress, microglial alteration, and immune imbalance in a mouse model of Werner syndrome

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