Infiltration of demineralized dentin with silica and hydroxyapatite nanoparticles

Besinis, Alexandros; Noort, Richard van; Martín, Nicolás

doi:10.1016/j.dental.2012.05.007

Cited by 104 publications

(82 citation statements)

References 43 publications

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“…In general terms, phosphate and carbonate peak heights and areas increased at the interface when NPs were used for dentin infiltration, but peaks and ratios concerning the organic components (crosslinking and nature/secondary structure of collagen) decreased after 21 d of immersion (Tables 3 and 4). Tested NPs had a higher diameter than the width of the collagen interfibrillar spaces, preventing NPs from passing into the hybrid layer (Besinis et al, 2012). However, when smaller particles (20 nm or less) are applied, infiltrative capability into the demineralized dentin is also reduced, due to particle agglomeration and/or to particle binding to demineralized collagen fibres (Besinis et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Tested NPs had a higher diameter than the width of the collagen interfibrillar spaces, preventing NPs from passing into the hybrid layer (Besinis et al, 2012). However, when smaller particles (20 nm or less) are applied, infiltrative capability into the demineralized dentin is also reduced, due to particle agglomeration and/or to particle binding to demineralized collagen fibres (Besinis et al, 2012). Particle binding to demineralized collagen was produced ( Figure 2B).…”

Section: Discussionmentioning

confidence: 99%

“…Particle binding to demineralized collagen was produced ( Figure 2B). It may be accounted for: 1) the result of the high affinity between the negatively charged polymeric NPs (-43.3 mV) and the positively charged demineralized dentin collagen (Besinis et al, 2012), or 2) due to the binding of COO -groups from NPs to NH + sites at dentin collagen. This step is crucial, as NPs collagen binding is necessary to exert a remineralization effect (Besinis et al, 2012(Besinis et al, , 2014.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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The aim of this study was to evaluate changes in the mechanical and chemical behavior, and bonding ability at dentin interfaces infiltrated with polymeric nanoparticles (NPs) prior to resin application. Dentin surfaces were treated with 37% phosphoric acid followed by application of an ethanol suspension of NPs, Zn-NPs or Ca-NPs followed by the application of an adhesive, Single Bond (SB). Bonded interfaces were stored for 24 h, submitted to microtensile bond strength test, and evaluated by scanning electron microscopy. After 24 h and 21 d of storage, the whole resin-dentin interface adhesive was evaluated using a Nano-DMA. Complex modulus, storage modulus and tan delta (δ) were assessed. AFM imaging and Raman analysis were performed. Bond strength was not affected by NPs infiltration. After 21 d of storage, tan δ generally decreased at Zn-NPs/resin-dentin interface, and augmented when Ca-NPs or non-doped NPs were used. When both Zn-NPs and Ca-NPs were employed, the storage modulus and complex modulus decreased, though both moduli increased at the adhesive and at peritubular dentin after Zn-NPs infiltration. The phosphate and the carbonate peaks, and carbonate substitution, augmented more at interfaces promoted with Ca-NPs than with Zn-NPs after 21 d of storage, but crystallinity did not differ at created interfaces with both ions-doped NPs. Crosslinking of collagen and the secondary structure of collagen improved with Zn-NPs resin-dentin infiltration. Ca-NPs-resin dentin infiltration produced a favorable dissipation of energy with minimal stress concentration trough the crystalline remineralized resin-dentin interface, causing minor damage at this structure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Toledano

Osorio

et al. 2017

Journal of the Mechanical Behavior of Biomedical Materials

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“…The authors observed that silica nanoparticles can penetrate dentin and remain embedded within the collagen matrix. The infiltration of demineralised dentin with sol-gel hydroxyapatite nanoparticles was limited but was significantly increased when combined with the deflocculating agent sodium hexametaphosphate and when acetone was used as transport vehicle 41 .…”

Section: Nanotechnology and Dental Applicationsmentioning

confidence: 99%

“…An alternative strategy that has become the focus of many studies in this field is the use of nanoparticles for the management of dental caries. Nano-sized calcium fluoride 36 , nano-sized carbonated apatite 37 , carbonate hydroxyapatite nanocrystals 38 , hydroxyapatite nanoparticles [39][40][41] and nanoparticulate bioactive glass 42 are nanomaterials that seem to increase the mineral content of enamel and dentin. Besinis et al 41 investigated the ability of colloidal silica and hydroxyapatite nanoparticles to infiltrate the collagen structure of demineralized dentin.…”

Section: Nanotechnology and Dental Applicationsmentioning

confidence: 99%

Nanotechnology in Dentistry

Arruda¹,

Oliveira²,

Paula³

et al. 2017

Arch Health Invest

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Introduction: Nanotechnology is a rapidly expanding field that encompasses the development, manipulation, and application of structures on the nanometer scale. Applications of nanotechnology to dentistry are particularly promising and comprise materials and devices designed to achieve maximal therapeutic efficacy with minimal side effects. Objective: This review discusses the advantages of nanotechnology and the different types of nanostructures used in dentistry. Material and Method: In this study, online databases: pubmed, medline and scielo were searched to analyse the current understanding of the potential of nanotechnology in dentistry, including the restoration of tooth structure with nanocomposites and the development of nanoparticles for dentin remineralisation, drug delivery, disease diagnostics, oral analgesia, oral hygiene maintenance, local anaesthesia, tooth desensitisation, and bone tissue repair. Results: The study demonstrated a wide range of nanotechnological strategies in different dentistry areas and suggests that nanotechnology-based delivery systems may be very useful to improve treatment, prevention and repair in dentistry in the future. Conclusion: There is little or no clinical experience for the nanotechnology-based drug delivery systems cited herein. Safety assessments and clinical trials are the next step in their development.

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The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

et al. 2022

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Biomimetic nanohydroxyapatite (nHAp) has long been used as a biocompatible material for bone repair, bone regeneration, and bone reconstruction due to its low toxicity to local or systemic tissues. Various cross‐linkers have been employed to maintain the structure of collagen; these include epigallocatechin‐3‐gallate (EGCG), which can fortify the mechanical properties of collagen and withstand the degradation of collagenase. We hypothesized that EGCG combined with nHAp may promote resin–dentin bonding durability. Here, we examined the effect of epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica (EGCG@nHAp@MSN) on thermal stability and remineralization capability of dentin collagen. Dentin slices (2 × 2 × 1 mm3) were obtained and completely demineralized in a 10% phosphoric acid water solution. The resulting dentin collagen matrix was incubated with deionized water, EGCG, nHAp@MSN, and EGCG@nHAp@MSN. The collagen thermal degradation temperature was assessed utilizing differential scanning calorimetry analysis, which indicated that EGCG, nHAp@MSN, and EGCG@nHAp@MSN reinforced collagen's capability to resist thermal degradation. EGCG@nHAp@MSN resulted in the highest increase in denaturation temperature. Thermogravimetric analysis showed that both nHAp@MSN and EGCG@nHAp@MSN achieved a higher residual mass than the EGCG and control groups. Fourier transform infrared spectroscopy was performed to examine the interaction between EGCG@nHAp@MSN and dentin collagen. The EGCG@nHAp@MSN sample exhibited stronger dentin microhardness and uppermost bond strength after thermocycling. EGCG significantly enhanced collagen's capability to resist thermal degradation. In summary, EGCG and nHAp@MSN may work together to assist the exposed collagen to improve resistance to thermal cycling and promote remineralization while also strengthening the durability of resin–dentin bonds.

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Infiltration of demineralized dentin with silica and hydroxyapatite nanoparticles

Cited by 104 publications

References 43 publications

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Ions-modified nanoparticles affect functional remineralization and energy dissipation through the resin-dentin interface

Nanotechnology in Dentistry

The durability of resin–dentine bonds are enhanced by epigallocatechin‐3‐gallate‐encapsulated nanohydroxyapatite/mesoporous silica

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