Infertility Induced in Mice by a Single Injection of Testosterone Propionate.

Barraclough, Charles A.; Leathem, James H.

doi:10.3181/00379727-85-20989

Cited by 101 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the demonstration by Barraclough & Leathern (1954) and by Barraclough (1958) that in mice and rats a single injection of androgen at the appro-M.R.C. Neuroendocrinology Unit, Department of Human Anatomy, South Parks Road, Oxford, England (Received 6 July 1970) priate time in early postnatal life produces permanent sterility, attention shifted to these species.…”

Section: Introductionmentioning

confidence: 98%

The 'Early Androgen Syndrome' in the Guinea-Pig

Brown-Grant¹,

Sherwood²

1971

Journal of Endocrinology

View full text Add to dashboard Cite

When testosterone propionate was administered to pregnant guinea-pigs over a short period (days 33-37) of gestation a high proportion of the female offspring exhibited a characteristic syndrome. The time of the first vaginal opening was delayed and its duration reduced. Subsequent periods of opening were fairly regular in occurrence but were shorter in duration than in normal animals; the 'cycle' length was usually slightly longer. Continuous vaginal opening was not observed but during the periods of opening, vaginal smears containing many cornified cells and no leucocytes were obtained. During the periods of vaginal opening no lordosis response to manual stimulation could be elicited nor did the animals mate when run with males. The increase in body weight was normal up to about 150 days of age and slightly, but not significantly, less than that of controls between 150 and 200 days of postnatal life. As adults some masculinization of the external genitalia was observed. At autopsy the weights of the uteri, ovaries, adrenal and anterior pituitary glands were much greater than those of control animals at any stage of the cycle. Histological examination showed that the ovaries contained many antral follicles but no luteal tissue. Enlargement of the glands and metaplastic changes in the epithelium were observed in the uteri. The pituitaries showed an excess of cells containing large, densely packed eosinophilic granules. This early androgen syndrome is compared with that produced by hypothalamic lesions in the guinea-pig and with the changes produced in other species by the administration of androgenic steroids during prenatal or early postnatal life.

show abstract

Section: Introductionmentioning

confidence: 98%

The 'Early Androgen Syndrome' in the Guinea-Pig

Brown-Grant¹,

Sherwood²

1971

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…That this hormonal interplay is critical for leukaemogenesis is illustrated by the experiment in which the SJL/J female mice were either gonadectomized (Table III) or in which a permanent sterilization and virilization were induced by one neonatal injection of testosterone (Table V). These procedures profoundly affect the mechanisms of synthesis and/or release of gonadotrophin-releasing factors in the hypothalamic centres, and permanently alter the cyclicity of females (Barraclough and Leathem, 1954;Barraclough, 1961a, b). It is certain that gonadotrophins (GTH) release is increased in both cases, and the findings are in conformity with the view of Gardner (1953) that hormonal carcinogenesis requires not only an ongoing hormonal imbalance, but also that cyclical or intermittent changes be replaced by continuous action.…”

Section: Discussionmentioning

confidence: 99%

“…(b) Masculinization of females.-Permanent masculinization and sterilization of SJL/J and CR females were induced by a single injection of 1 mg testosterone propionate in peanut oil, between 1 and 3 days of age (Barraclough and Leathem, 1954;Barraclough, 1961a, b). Controls were injected with oil only.…”

Section: Animalsmentioning

confidence: 99%

Role of host endocrine status in murine leukaemogenesis

Pierpaoli¹,

Haran-Ghera²,

Kopp³

1977

Br J Cancer

View full text Add to dashboard Cite

Summary.-Permanent changes in the endocrine status of female SJL/J and CR mice were induced by masculinization, ablation of endocrine glands, inoculation of hormones, or feeding of the chemical carcinogen DMBA. All these prodecures resulted in modification of the host hormonal milieu, as shown by blood hormone determination. Masculinization reduced drastically the onset of lymphosarcoma and increased the incidence of systemic neoplasms respectively in DMBA-treated female SJL/J and CR mice. Continued administration of gonadotrophins increased the incidence of systemic neoplasia in CR mice. A direct correlation is suggested between onset of lymphosarcoma or other tumours in mice and a specific shift to an abnormal hormonal environment.

show abstract

“…It was shown by Barraclough & Leathem (1954) and Barraclough (1961) that a single dose of testosterone given to 5-day-old mice and rats was enough to inhibit normal sexual development, and that this date seemed to be the most critical moment after birth for determination of normal development. Previously, repeated doses of testosterone had been given over considerable periods.…”

Section: Discussionmentioning

confidence: 99%

The vascular responses to neurohypophysial hormones of rats and dogs with abnormal sexual development

Lloyd¹,

Pickford²

1963

The Journal of Physiology

View full text Add to dashboard Cite

When studying afresh the actions of oxytocin on the cardiovascular system it was found to be a vasodilator in normal circumstances, but a vasoconstrictor following either sympathetic block or the administration of oestrogens (Lloyd, 1959;Lloyd & Pickford, 1961. On those occasions when oxytocin became pressor the sensitivity to vasopressin (Pitressin, Parke Davis) was increased. The importance of the gonadal hormones in determining cardiovascular reactivity was further shown in that, following gonadectomy in the rat, temporary changes in the response to oxytocin and vasopressin occurred (Lloyd, 1959; Honore6 & Lloyd, 1961), with the return of an apparently normal response to oxytocin and a slightly modified one to vasopressin by the 10th day after operation. The aim of the work described in this paper was to study the effect of the neurohypophysial hormones on the blood pressure of animals whose sexual development was disturbed or inhibited as soon as practicable after birth. METHODSThe animals used were twelve hooded rats and four dogs. The rats came from two litters. At 5 days of age the males were given 2-3 mg stilboestrol dipropionate in oil subcutaneously in a total volume of 0-2-0-3 ml. and the females received at the same age 1-25 mg testosterone propionate in oil subcutaneously in a total volume of 0-125 ml. The animals were used experimentally when between 3 and 4 months old, by which time the males weighed from 225 to 360 g and the females from 200 to 260 g. After death the testes and ovaries were examined histologically. The ovaries contained a number of follicles and no corpora lutea.The testes were small and contained few spermatozoa when compared with normal ones; in particular few spermatozoa were in the lumen of the tubules. Anaesthesia was induced with intraperitoneal sodium pentobarbitone (5 mg/100 g body weight). Blood pressure was recorded by mercury manometer on smoked paper through an intracarotid cannula, and injections were given through a cannula inserted into a femoral vein. Vaginal smears were examined intermittently in the females and were always of the oestrous type.Of the four dogs used, two were males and two females, three of the puppies being from one litter and one from another. At the age of 6 weeks, at aseptic operations, one male was castrated extra-abdominally, and one female ovariectomized through a mid-line abdominal incision. All four dogs grew up at about the same period in similar conditions and on similar diets. All were used experimentally when between 11-5 and 13 months of age. Anaesthesia

show abstract

Infertility Induced in Mice by a Single Injection of Testosterone Propionate.

Cited by 101 publications

References 0 publications

The 'Early Androgen Syndrome' in the Guinea-Pig

The 'Early Androgen Syndrome' in the Guinea-Pig

Role of host endocrine status in murine leukaemogenesis

The vascular responses to neurohypophysial hormones of rats and dogs with abnormal sexual development

Contact Info

Product

Resources

About