Infertility in the Aging Male

Mazur, Daniel J.; Lipshultz, Larry I.

doi:10.1007/s11934-018-0802-3

Cited by 51 publications

(50 citation statements)

References 87 publications

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“…The semen analysis seems to be crucial for assessing the fertility potential of males. The comparisons of semen parameters of younger and older men in individual studies demonstrated inconsistent findings, while meta-analyses proved that age has a negative impact on semen quality, including volume, morphology, and motility (Mazur and Lipshultz, 2018). The present study showed that study participants with higher motility were significantly younger than those with lower motility.…”

Section: Discussioncontrasting

confidence: 50%

Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen

et al. 2020

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Infertility is among the most serious medical problems worldwide. Male factors contribute to 40%–50% of all infertility cases, and approximately 7% of men worldwide are affected by infertility. Spermatozoa are extremely vulnerable to oxidative insult. Oxidative stress results in axonemal damage and increased midpiece sperm morphological defects, which lead to reduced sperm motility. The aim of the study is to evaluate the association between sperm motility and the levels of selected antioxidants, cytokines, and markers of oxidative damage in the seminal plasma. The study group included 107 healthy males, who were split into two subgroups based on the percentage of motile spermatozoa after 1 hr: low motility (LM, n = 51) and high motility (HM, n = 56). The glucose-6-phosphate dehydrogenase (G6PD) activity was 52% lower in the LM group compared to that in the HM group. The level of malondialdehyde (MDA) was 12% higher in the LM group compared to that in the HM group. Similarly, the median values of interleukin (IL)-1β, IL-10, IL-12, and tumor necrosis factor alpha (TNF-α) were higher in the LM group than those in the HM group. Results of the present study revealed that the percentage of motile spermatozoa after 1 hr correlated positively with the levels of IL-1β, IL-10, IL-12, and TNFα. The lower motility of spermatozoa in healthy men is associated with a decreased activity of G6PD and increased levels of cytokines, which may be related to increased oxidative stress in seminal plasma that manifests as an increased level of MDA.

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Section: Discussioncontrasting

confidence: 50%

Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen

et al. 2020

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“…Ageing or other diseases have a negative influence on spermatogenic function and fertility (Cardona Maya, Berdugo, & Cadavid Jaramillo, ; Mazur & Lipshultz, ; Rybar, Kopecka, Prinosilova, Markova, & Rubes, ; Sekhavat & Moein, ; Varshini et al, ). In this study, we found that in elderly human testes, spermatogenic function declines were characterised by histological atrophic lesions and thin seminiferous epithelium, lower Johnsen's scores and decreased expression of spermatogenic cells markers, including SSC (PLZF + ), differentiating spermatogonia (C‐kit + ), spermatocyte (PIWIL1 + ) and spermatid (SMAD3 + ) (Fayomi & Orwig, ; Hermann et al, ; Sauer, ).…”

Section: Discussionmentioning

confidence: 99%

Phosphorylated mixed lineage kinase domain‐like protein in human seminal plasma: A potential novel biomarker of spermatogenic function

Xie

Yao

et al. 2019

Andrologia

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Keeping the self-renewal and differentiation of spermatogonial stem cell (SSC) in balance is essential for maintaining spermatogenesis. However, whether the cell death of SSC also plays a vital role in the human being remains unknown. To explore the necroptosis of SSC, the activation marker of necroptosis, phosphorylated mixed lineage kinase domain-like protein (pMLKL) in testes was evaluated by immunofluorescence staining and Western blot. Meanwhile, a total of 81 semen samples were divided based on the sperm concentration (>15, 10-15, 5-10 and 0-5 million/ml) to study the relationships between pMLKL levels and sperm counts. We found that the pMLKL was increased in the ageing human testes (p < 0.05). Moreover, the seminal pMLKL expression was decreased in groups with sperm concentration 0-5 and 5-10 million/ml when compared with normal sperm concentration in young men (p < 0.05). Further analysis revealed that pMLKL showed an age-related increased expression in men aged 22-60 years with normal sperm concentration. These data demonstrated that the necroptosis of SSC was important for the spermatogenic function and would raise in advance on the point of testicular hypofunction. In conclusion, the pMLKL may serve as a potential biologically seminal indicator for the spermatogenic function in men.

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“…Women age is a major risk factor (Fritz & Jindal, ; Shirasuna & Iwata, ), with fertility even after in vitro fertilisation (IVF) dropping below 30% already by 35 years of age (Q‐IVF, ). Male subfertility at similar age intervals is thought to be predisposed/caused by several factors all leading to low sperm count, sperm dysfunction or both (Levine et al, ; Mazur & Lipshultz, ).…”

Section: Introductionmentioning

confidence: 99%

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?

et al. 2019

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Men affected with idiopathic infertility often display basic spermiogramme values similar to fertile individuals, questioning the diagnostic impact of the World Health Organization (WHO) thresholds used. This study explored sperm DNA fragmentation in single ejaculates from 14 fertile donors and 42 patients with idiopathic infertility providing semen for assisted reproductive techniques in a university fertility clinic. Each ejaculate was simultaneously studied for sperm DNA fragmentation by the flow cytometer‐based sperm chromatin structure analysis (SCSA) and the new light‐microscopy‐based sperm chromatin dispersion assay (SCD‐HaloSpermG2®), before and after sperm selection for in vitro fertilisation with a colloid discontinuous gradient. The WHO semen variables did not differ between groups, but DNA fragmentation after SCSA (DFI) or SCD (SDF) was significantly (p < 0.05) higher in patients (DFI: 40.2% ± 3.0 vs. SDF: 40.3% ± 1.4) than in fertile donors (DFI: 17.1% ± 2.1 vs. SDF: 20.9% ± 2.5). Sperm selection led to lower proportions of DNA‐fragmented spermatozoa (DFI: 11.9 ± 1.7 vs. SCD: 10.0 ± 0.9, p < 0.05). The techniques output correlated highly and significantly (r2 = 0.82). DNA fragmentation is confirmed as a relevant variable for scrutinising patients with idiopathic infertility, beyond the evidently insufficient WHO semen analyses. Since both techniques yielded similar results, the reduced necessity of complex equipment when running SCD ought to be considered for a clinical setting.

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Infertility in the Aging Male

Cited by 51 publications

References 87 publications

Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen

Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen

Phosphorylated mixed lineage kinase domain‐like protein in human seminal plasma: A potential novel biomarker of spermatogenic function

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?

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Infertility in the Aging Male

Cited by 51 publications

References 87 publications

Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen

Oxidative Stress is Associated with Reduced Sperm Motility in Normal Semen

Phosphorylated mixed lineage kinase domain‐like protein in human seminal plasma: A potential novel biomarker of spermatogenic function

Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ® ) comparable?

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Diagnostics of DNA fragmentation in human spermatozoa: Are sperm chromatin structure analysis and sperm chromatin dispersion tests (SCD‐HaloSpermG2 ^® ) comparable?