Inferring the evolutionary relationship of 23 Malaysian Rhodococcus isolates with potential as cholesterol degrading bacteria

Hasdianty, A.; Suhaila, Y. Nor; Hazwan, A. Hazeeq; Maniyam, Maegala Nallapan; Fadzli, A. Mohd; Ibrahim, Ab Latif

doi:10.1016/j.bcab.2020.101840

Cited by 10 publications

(3 citation statements)

References 42 publications

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“…R. pyridinivorans strain UCC 0003 (Hasdianty et al, 2020 ) was isolated from a sludge sample from Kuala Lukut, Negeri Sembilan, Malaysia, and kindly provided by the Culture Collection Unit of Institute of Bio-IT Selangor, Universiti Selangor. This strain has been deposited in GenBank ® (NCBI) under the gene accession number MN56068.…”

Section: Methodsmentioning

confidence: 99%

Optimization of culture conditions for improved green biodecolourization of methylene blue by Rhodococcus pyridinivorans strain UCC 0003

Maniyam

Hari

2021

bta

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Methylene blue is a toxic dye present in the textile industry, and if left untreated, it causes harm to the environment. Therefore, to decolorize methylene blue from industrial effluents, a green approach using Rhodococcus pyridinivorans strain UCC 0003 was attempted. Methylene blue decolorization was measured spectrophotometrically, and the static condition yielded 86% decolorization after 24 h as compared to the shaking mode (20%). Optimization of static conditions using the one-factor-at-a-time approach resulted in 100% decolorization at 30EC, pH 6, inoculum size of 16% (v/v), and 5% (v/v) banana peel addition as a carbon source. The R. pyridinivorans strain UCC 0003 could successfully and completely decolorize 0.75 g/l methylene blue for 4 consecutive cycles, which is advantageous from an economic point of view. The rate of methylene blue disappearance was investigated using 10% (v/v) R. pyridinivorans strain UCC 0003 at 30EC over a certain incubation time with 0.4 g/l to 10.0 g/l methylene blue as the substrate. This study revealed V max and K m values of 37.04 g/l/h and 55.69 g/l, respectively, as the kinetic behavior of methylene blue-decolorizing enzymes from the bacterial strain. The properties of the treated solution of methylene blue resembled the control system (distilled water) for the phytotoxicity study, thereby indicating the complete removal of dye toxicity as evidenced by the growth of Vigna radiata and Triticum aestivum, respectively, in the treated methylene blue solution. This local bacterial strain has therefore a huge potential to be used as a green biocatalyst for the bioremediation of methylene blue-containing industrial effluents.

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Section: Methodsmentioning

confidence: 99%

Optimization of culture conditions for improved green biodecolourization of methylene blue by Rhodococcus pyridinivorans strain UCC 0003

Maniyam

Hari

2021

bta

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“…This strain was routinely conserved and stored at the Culture Collection Unit of Institute of Bio-IT Selangor, Universiti Selangor. This strain was placed at the GenBank ® (NCBI) and identified with an accession number MN56069 [15].…”

Section: Isolation Of R Pyridinivoransmentioning

confidence: 99%

Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis

Maniyam¹,

Hazwan²,

Abdullah³

et al. 2022

J App Biol biotech

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Environmental concern over the discharge of improperly treated textile wastewater has been on the rise. The present study explored the use of the limitedly studied, tropical Rhodococcus pyridinivorans for the decolorization of crystal violet, a typically encountered dye in the textile industry. The effect of agitation speed, temperature, pH, nutritional source, initial dye concentration, and size of inoculum on crystal violet removal were evaluated using one-factorat-a-time method. Decolorization of 0.6 mM crystal violet carried out at agitated mode showed 73 ± 3% crystal violet removal efficiency in comparison to static mode (43 ± 2%) after 24 h. This crystal violet removal efficiency escalated to 91 ± 2% after optimizing the culture conditions at 45°C and pH 7 using maltose as carbon source and 12% (v/v) size of inoculum. Furthermore, the optimization process reduced the incubation time to achieve almost complete decolorization by 67%. Ultraviolet-visible analysis revealed that the decolorization of crystal violet primarily occurred through biodegradation. The findings from this study validated the potential of R. pyridinivorans as an effective biocatalyst to remediate crystal violet. R. pyridinivorans will be attempted to decolorize different triphenylmethane dyes, namely, malachite green in future studies.

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“…Rhodococcus pyridinivorans strain UCC 0009 with a gene accession number of MN56068 [11] was obtained from the culture collection unit, Universiti Selangor. The strain was streaked on nutrient agar plates (20 g/L) and the plates were kept in an incubator (MEMMERT 108L Incubator INB500, Germany) at 30 °C for 36 hours.…”

Section: Preparation Of Seed Culturementioning

confidence: 99%

Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

et al. 2021

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Recently, the application of lipase enzyme as biocatalyst in the conversion of waste cooking oil (WCO) to free fatty acids and glycerol has been trending well. Therefore, the present study attempts to use WCO which is found in abundance in Malaysia as the substrate for halal microbial lipase conversion to glycerol which can be exploited in the food industry. The workability of free lipase for WCO conversion, however suffers severely due to potential denaturation of the enzyme and extended reaction time. Thus, this study embraced the immobilization method to encapsulate crude lipase extracted from Rhodococcus pyridinivorans strain UCC 0009 in gellan gum and calcium alginate, respectively and compared their ability for WCO conversion to free crude lipase. The gellan gum and calcium alginate-immobilized crude lipase evidently exhibited greater WCO conversion, demonstrating 2.18-fold and 1.61-fold enhanced lipase activity, respectively in comparison to free crude lipase. The repeated reuse of the gellan gum-immobilized crude lipase maintained reasonable lipase activity for 9 cycles, retaining an average 85 % WCO conversion for the first seven cycles and 67 % conversion in the subsequent batches. Thus, the immobilized halal lipase can be foreseen as a green substitute to chemical catalyst for WCO conversion which meets the worldwide demand for clean technologies.

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Inferring the evolutionary relationship of 23 Malaysian Rhodococcus isolates with potential as cholesterol degrading bacteria

Cited by 10 publications

References 42 publications

Optimization of culture conditions for improved green biodecolourization of methylene blue by Rhodococcus pyridinivorans strain UCC 0003

Optimization of culture conditions for improved green biodecolourization of methylene blue by Rhodococcus pyridinivorans strain UCC 0003

Process optimization for efficacious biodecolorization of crystal violet by Malaysian Rhodococcus pyridinivorans using monothetic analysis

Conversion of waste cooking oil by rhodococcal lipase immobilized in gellan gum

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