Infectivity of turnip mosaic potyvirus cDNA clones and transcripts on the systemic host Arabidopsis thaliana and local lesion hosts

Sánchez, Flora; Martínez-Herrera, David I.; Aguilar, Isabel; Ponz, F

doi:10.1016/s0168-1702(98)00049-5

Cited by 85 publications

(73 citation statements)

References 46 publications

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“…The Col-0 ecotype (RTM1) is fully susceptible to a range of other viruses, such as turnip crinkle virus, turnip mosaic virus, and cauliflower mosaic virus (5,32,33). Does the RTM1 gene confer TEV specificity, or does RTM1 participate in a complex or pathway in which specificity is determined by another component?…”

Section: Discussionmentioning

confidence: 99%

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Chisholm

Mahajan

Whitham

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

191

102

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The locus RTM1 is necessary for restriction of long-distance movement of tobacco etch virus in Arabidopsis thaliana without causing a hypersensitive response or inducing systemic acquired resistance. The RTM1 gene was isolated by map-based cloning. The deduced gene product is similar to the ␣-chain of the Artocarpus integrifolia lectin, jacalin, and to several proteins that contain multiple repeats of a jacalin-like sequence. These proteins comprise a family with members containing modular organizations of one or more jacalin repeat units and are implicated in defense against viruses, fungi, and insects.resistance

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Section: Discussionmentioning

confidence: 99%

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Chisholm

Mahajan

Whitham

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

191

102

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“…A. tumefaciens strain GV2260 with binary TuMV constructs were first used to infect N. benthamiana and subsequent infectious TuMV extracts from N. benthamiana were used to infect A. thaliana plants as described in ref. 13. Mottling of young A. thaliana leaves was observed after 6 -8 days.…”

Section: Methodsmentioning

confidence: 99%

“…Turnip mosaic virus (TuMV) is able to systemically infect A. thaliana (12,13). The virus belongs to the class of single-stranded (ϩ) RNA potyviruses, with nonoverlapping ORFs (Fig.…”

Section: ⌬41atr13 Emco5 Activates Resistance To a Viral Pathogen In Pmentioning

confidence: 99%

Recognition of the Hyaloperonospora parasitica effector ATR13 triggers resistance against oomycete, bacterial, and viral pathogens

Rentel

Leonelli

Dahlbeck

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

114

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Phytopathogenic oomycetes cause some of the most devastating diseases affecting agricultural crops. Hyaloperonospora parasitica is a native oomycete pathogen of Arabidopsis and is related to other oomycete phytopathogens that include several species of Phytophthora, including the causal agent of potato late blight. Recently, four oomycete effector genes have been isolated, and several oomycete genomes have been sequenced. We have developed an efficient and genetically amenable system to test putative effector genes using the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. The H. parasitica effector protein ATR13 was delivered via P. syringae by fusing the ATR13 gene with the avrRpm1 type three secretion signal peptide, a bacterial sequence that allows transfer of proteins into the host cell through the bacterial type III secretion system. We also inserted ATR13 into the genome of the turnip mosaic virus, a single-stranded RNA virus. Our results show that delivery of ATR13 via the bacterial or viral pathogen triggers defense responses in plants containing the cognate resistance protein RPP13 Nd , which restricts proliferation of both pathogens. Hence, recognition of ATR13 by RPP13 initiates defense responses that are effective against oomycete, bacterial and viral pathogens, pointing to a common defense mechanism. We have characterized regions of the RPP13 Nd resistance protein that are essential for effector recognition and/or downstream signaling, using transient coexpression in Nicotiana benthamiana.plant disease resistance ͉ effector-triggered plant immunity ͉ plant pathogenesis

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“…Importantly, TuMV infects Arabidopsis thaliana, the well known plant model (Martínez-Herrera et al, 1994). Infectious cloned cDNAs of TuMV have been previously described (Sánchez et al, 1998), which are increasingly being used as tools in studies of plant-virus interactions. This paper reports the derivation of the original TuMV infectious clone, p35Tunos, into a vector (p35Tunos-vec01), useful for the expression of heterologous genes in TuMV hosts.…”

Section: Resumen Altos Niveles De Expresión De Proteínas Foráneas a Pmentioning

confidence: 99%

“…An infectious cDNA clone of TuMV, p35Tunos (Sánchez et al, 1998) was modified to introduce a NgoMIV restriction site at the NIb-CP junction. There are no NgoMIV restriction sites in p35Tunos.…”

Section: Construction Of P35tunos-vec01mentioning

confidence: 99%

High expression of foreign proteins from a biosafe viral vector derived from Turnip mosaic virus

Touriño

Sánchez

Fereres

et al. 2008

Span. j. agric. res.

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A vector derived from an original infectious clone of Turnip mosaic potyvirus (TuMV) has been developed. The vector (p35Tunos-vec01) was made through the creation of a new unique insertion site for foreign genes between the NIb and the CP genes of the viral genome. The jellyfish green fluorescent protein (GFP) and E. coli beta-glucuronidase (GUS) were expressed in Arabidopsis thaliana plants infected with chimeric vectors carrying their corresponding genes. In the case of GUS, expression levels that were 15-50 fold higher than nuclear transgenic A. thaliana lines carrying the same gene were attained. Acceptable levels of gene stability were found in the infected plants, compatible with the use of the vector for protein production in plants. A non-aphid transmissible version of the vector was also made and its lack of aphid transmission was extensively shown. The vector was not transmitted through seeds or by contact between plants.

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Infectivity of turnip mosaic potyvirus cDNA clones and transcripts on the systemic host Arabidopsis thaliana and local lesion hosts

Cited by 85 publications

References 46 publications

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Cloning of the Arabidopsis RTM1 gene, which controls restriction of long-distance movement of tobacco etch virus

Recognition of the Hyaloperonospora parasitica effector ATR13 triggers resistance against oomycete, bacterial, and viral pathogens

High expression of foreign proteins from a biosafe viral vector derived from Turnip mosaic virus

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