INFECTIVITY OF Oryctes NUDIVIRUS PRODUCED ON CELL CULTURE DSIR HA-1179 AGAINST LARVAE AND ITS EFFECTS ON FEEDING OF NEONATES OF RHINOCEROS BEETLE, Oryctes rhinoceros

Khudri, Nur Ain Farhah Ros Saidon

doi:10.21894/jopr.2016.2803.03

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…The survival rate of OrNV‐infected larvae gradually decreased to 92% at 12 days post‐infection (pids), 50% at 27 pids and no survival at 34 pids. This result is in agreement with a previous study that reported 50% survival of OrNV‐infected larvae at 20 pids and no survival at 30 pids (Khudri et al 2016). However, the survival rate of control larvae inoculated with PBS did not start to decrease until 20 pids and remained at 65% until 34 pids.…”

Section: Resultssupporting

confidence: 94%

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Cho,

Vaidya,

Kwon

et al. 2023

Entomological Research

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Oryctes rhinoceros nudivirus (OrNV) infects the larval stage of many coleopteran insects; however, the underlying mechanisms and biomarkers of infection are not fully characterised. In this study, an optimal culture condition was developed for OrNV replication and proteomic biomarkers were identified using comparative proteomic analysis. The highest level of viral copy number was observed in Sf9 cells treated with 450 μM of H2O2 and 2% foetal bovine serum (FBS). Among the 48 identified proteins, 14 proteins were significantly modulated in 2% FBS and H2O2‐ treated OrNV‐infected cells (F2V) as compared with 10% FBS treated non‐infected cells (F10M). Network analysis revealed that SLC25A5, VDAC3, PHB2, and ANXA1 act as signature proteins for OrNV replication. Moreover, viral envelope glycoproteins, GRBNV_gp28‐like and GrBNV_gp62‐like proteins could be used as sensitive diagnostic signatures for OrNV infection. Furthermore, to conveniently identify the OrNV‐infection in Allomyrina dichotoma larvae, an image classification model was trained by Google Teachable Machine, which distinguished images with accuracy rates of 91% and 86% for infected and non‐infected larvae, respectively, at a learning rate of 0.001. This study demonstrated that Sf9 cell medium treated with 2% FBS and 450 μM H2O2 is a permissible culture condition for OrNV replication. Proteomic signatures may be involved in the progression of viral infection. Additionally, a low‐cost and non‐invasive machine learning‐derived digital imaging analysis may improve the prediction of OrNV infection in larvae.

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Section: Resultssupporting

confidence: 94%

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Cho,

Vaidya,

Kwon

et al. 2023

Entomological Research

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Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)

Bedford¹

2018

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It is suggested that the isolates of Oryctes Nudivirus (OrNV), cultured for decades in cells of Heteronychus arator (F.) (HA), be checked to verify genomic changes have not occurred which adapt them to culture but reduce or cancel their ability to infect the target pest, the coconut rhinoceros beetle (CRB), Oryctes rhinoceros (L.). Full genomes of field-caught OrNV isolates, and their infectivity against larvae and adults, could be compared with those of HA-cultured isolates. Further data to correlate OrNV dosage indices with doses in number of virions/ml could be advantageous so as to explore if CRB larvae or adults may resist infection by a sub-threshold dose. Also the possibility of changes in the HA culture cells which alter the outer coat of the resulting virion, hence perhaps its infectivity towards CRB cells, could be checked. Might it be possible to move beyond HA-culture and develop tissue culture of Oryctes rhinoceros cells for mass production of OrNV as this beetle species is the target? Nuclear genomes of OrNV-resistant and OrNV-susceptible strains of the CRB could be examined for changes perhaps correlated with resistance. The possibility of endosymbiotic bacteria affecting CRB susceptibility to OrNV might be checked.

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INFECTIVITY OF Oryctes NUDIVIRUS PRODUCED ON CELL CULTURE DSIR HA-1179 AGAINST LARVAE AND ITS EFFECTS ON FEEDING OF NEONATES OF RHINOCEROS BEETLE, Oryctes rhinoceros

Abstract: ABSTRACT

Cited by 2 publications

References 13 publications

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)

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INFECTIVITY OF Oryctes NUDIVIRUS PRODUCED ON CELL CULTURE DSIR HA-1179 AGAINST LARVAE AND ITS EFFECTS ON FEEDING OF NEONATES OF RHINOCEROS BEETLE, Oryctes rhinoceros

Abstract: ABSTRACT

Cited by 2 publications

References 13 publications

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Exploration of proteomic biomarkers and digital imaging analysis for Oryctes rhinoceros nudivirus infection

Possibility of Evolution in Culture of the &lt;i&gt;Oryctes&lt;/i&gt; Nudivirus of the Coconut Rhinoceros Beetle &lt;i&gt;Oryctes rhinoceros&lt;/i&gt; (Coleoptera: Scarabaeidae: Dynastinae)

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Possibility of Evolution in Culture of the <i>Oryctes</i> Nudivirus of the Coconut Rhinoceros Beetle <i>Oryctes rhinoceros</i> (Coleoptera: Scarabaeidae: Dynastinae)