Infectivity of human group B adenovirus to cells from different species

Abstract

Adenovirus is a world public health problem that causes symptoms of respiratory, intestine, urethra and bladder, eyes and liver. The aim of this study is to observe the infectivity of adenovirus to cells from different species, which will provide evidence for finding potential adenovirus infecting animals and nature hosts. 10 cells from different species were infected with human adenovirus (HAdV) in group B (HAdV-3, HAdV-7, HAdV-14 and HAdV-55). Results showed that all the cells supported HAdV-55 entry and hexon protein expression. Increased viral DNA levels were observed in HEp-2, Vero, MDCK, PK15, OAR-L1, MDBK, and F81 cells, while not in CHO, NIH3T3, CT26WT and BHK21 cells. Infectious viral particles were produced in HAdV-55 infected HEp-2, Vero, MDCK, MDBK and PK15 cells, and in HAdV-7 infected HEp-2, Vero, MDCK, F81, MDBK, PK15 and OAR-L1 cells. Conclusively, it is possible to find animals that might be infected by human adenovirus through screening of susceptible cells, and the prevalence of human adenovirus in these animals deserves further investigation.