Infectivity of Epiphyas postvittana nucleopolyhedrovirus for New Zealand leafrollers

Markwick, Ngaire P.; Graves, Sally; Fairbairn, Fiona M; Docherty, Lisa C.; Poulton, Joanne; Ward, Vernon K.

doi:10.1016/s1049-9644(02)00059-2

Cited by 7 publications

(13 citation statements)

References 27 publications

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“…In Japan, the use of B. mori and BmNPV has also yielded good results. Other combinations may prove better, for example the Australian tortricid, Epiphyas postvittana, and its associated virus, EppoNPV [25] may be useful because this virus develops more slowly [26] than AcMNPV and BmNPV, and may permit greater continued expression before the insect needs to be harvested.…”

Section: Discussionmentioning

confidence: 99%

Expression of two heterologous proteins depends on the mode of expression: comparison of in vivo and in vitro methods

Gatehouse

Markwick

Poulton

et al. 2008

Bioprocess Biosyst Eng

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The yield of two proteins, avidin and green fluorescent protein (GFP), expressed from a modified Autographa californica nucleopolyhedrovirus (AcMNPV), was compared in Sf9 cell culture monolayer, Sf21 cell suspension culture and intact Spodoptera litura larvae. GFP expressed from the p10 promoter yielded up to 1.5% of total soluble protein in larvae, 20-fold higher than that in monolayer suspension culture. Avidin, expressed from the polh promoter, yielded up to 2.3% of total soluble protein in larvae, 10-fold higher than that in suspension culture and 40-fold higher than that in monolayers. Avidin expression did not affect amounts of GFP in dual-expressing baculovirus compared with those detected from a GFP-only expressing AcMNPV. A biotin-binding assay showed that all avidin expressed in larvae was fully active. Glycosylation patterns of chicken-avidin and Spodoptera-avidin were very similar, though the latter showed a proportion of partially glycosylated material.

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Section: Discussionmentioning

confidence: 99%

Expression of two heterologous proteins depends on the mode of expression: comparison of in vivo and in vitro methods

Gatehouse

Markwick

Poulton

et al. 2008

Bioprocess Biosyst Eng

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“…In New Zealand apple orchards, control of E. postvittana has typically been achieved using insecticides, but resistance has arisen (Armstrong & Suckling, 1988). Use of Bt (McKenna, 1998) and EppoNPV (Markwick et al, 2002) have been investigated as well as potential insecticides such as avidin (Markwick et al, 2002). The effective use of these and novel control strategies will require a thorough knowledge of digestive physiology and identification of target genes.…”

Section: Introductionmentioning

confidence: 99%

Expressed sequence tags from the midgut of Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae)

Simpson

Newcomb

Gatehouse

et al. 2007

Insect Molecular Biology

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The midgut is a key tissue in insect science. Physiological roles include digestion and peritrophic membrane function, as well as being an important target for insecticides. We used an expressed sequence tag (EST) approach to identify candidate genes and gene families involved in these processes in the light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae). Two cDNA libraries were constructed from dissected midgut of third to fifth instar larvae. Clustering analysis of 6416 expressed sequence tags produced 1178 tentative unique genes comprising 725 tentative contigs and 453 singletons. The sequences show similar codon usage to sequences from other lepidopterans, a Kozak consensus sequence similar to Drosophila and single nucleotide polymorphisms (SNPs) were detected at a frequency of 1.35/kb. The identity of the most common Interpro families correlates well with major known functions of the midgut. Phylogenetic analysis was conducted on representative sequences from selected multigene families. Gene families include a broad range of digestive proteases, lipases and carbohydrases that appear to have degradative capacity against the major food components found in leaves, the diet of these larvae; and carboxylesterases, glutathione-S-transferases and cytochrome P450 monooxygenases, potentially involved in xenobiotic degradation. Two of the larger multigene families, serine proteases and lipases, expressed a high proportion of genes that are likely to be catalytically inactive.

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“…Initial attempts to recover EppoNPV in the EpN1 cell line at 27°C yielded a limited number of infected cells. Because in vivo propagation of EppoNPV is undertaken at 21°C (Markwick et al, 2002), a reduction in culture temperature was investigated in the EpN1 and EpN1.10 cells (Fig. 1B).…”

Section: Clonal Derivatives Of the Epn1 Cell Line And Establishment Omentioning

confidence: 99%

“…Epiphyas postvittana nucleopolyhedrovirus (EppoNPV: Baculoviridae) was identified in Australia and New Zealand in the 1960s (Longworth and Singh, 1980;Thomas, 1965) and infects E. postvittana, and other members of the leafroller complex (Markwick et al, 2002). The inability of EppoNPV to infect non-target insects and promise in early field trials (Longworth, 1976) suggests EppoNPV has potential as a biocontrol agent.…”

Section: Introductionmentioning

confidence: 99%

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Establishment of a neonate cell line from Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae) that supports replication of E. postvittana nucleopolyhedrovirus

Young

Sneddon

Ward

2010

Journal of Invertebrate Pathology

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Infectivity of Epiphyas postvittana nucleopolyhedrovirus for New Zealand leafrollers

Cited by 7 publications

References 27 publications

Expression of two heterologous proteins depends on the mode of expression: comparison of in vivo and in vitro methods

Expression of two heterologous proteins depends on the mode of expression: comparison of in vivo and in vitro methods

Expressed sequence tags from the midgut of Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae)

Establishment of a neonate cell line from Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae) that supports replication of E. postvittana nucleopolyhedrovirus

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