Infectious entry pathway for canine parvovirus

Basak, Sukla; Turner, Henrietta

doi:10.1016/0042-6822(92)90002-7

Cited by 57 publications

(46 citation statements)

References 28 publications

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“…However, it seems that interactions between hydrophobic portions of capside proteins participate in the entry. Furthermore, the entry is pH dependent, because endosome acidification blocks virus infection and replication (67).…”

Section: Discussionmentioning

confidence: 99%

In Vivo, Dendritic Cells Can Cross-Present Virus-Like Particles Using an Endosome-to-Cytosol Pathway

Morón

Rueda

Sedlik

2003

The Journal of Immunology

102

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Recombinant parvovirus-like particles (PPV-VLPs) are particulate exogenous Ags that induce strong CTL response in the absence of adjuvant. In the present report to decipher the mechanisms responsible for CTL activation by such exogenous Ag, we analyzed ex vivo and in vitro the mechanisms of capture and processing of PPV-VLPs by dendritic cells (DCs). In vivo, PPV-VLPs are very efficiently captured by CD8α− and CD8α+ DCs and then localize in late endosomes of DCs. Macropinocytosis and lipid rafts participate in PPV-VLPs capture. Processing of PPV-VLPs does not depend upon recycling of MHC class I molecules, but requires vacuolar acidification as well as proteasome activity, TAP translocation, and neosynthesis of MHC class I molecules. This study therefore shows that in vivo DCs can cross-present PPV-VLPs using an endosome-to-cytosol processing pathway.

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Section: Discussionmentioning

confidence: 99%

In Vivo, Dendritic Cells Can Cross-Present Virus-Like Particles Using an Endosome-to-Cytosol Pathway

Morón

Rueda

Sedlik

2003

The Journal of Immunology

102

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“…Blocking acidification of endosomes is a way of showing entry of virus consistent with clathrin-mediated endocytosis (Basak & Turner, 1992). We investigated the role of endosomal acidification in BPV entry into EBTr cells employing NH 4 Cl, chloroquine (CHLO) and bafilamicin A1 (Baf A1).…”

Section: Bpv Entry Into Cells Utilizes Clathrin and Endosomal Acidifimentioning

confidence: 99%

Bovine parvovirus uses clathrin-mediated endocytosis for cell entry

Dudleenamjil¹,

Lin²,

Dredge³

et al. 2010

Journal of General Virology

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Entry events of bovine parvovirus (BPV) were studied. Transmission electron micrographs of infected cells showed virus particles in cytoplasmic vesicles. Chemical inhibitors that block certain aspects of the cellular machinery were employed to assess viral dependency upon those cellular processes. Chlorpromazine, ammonium chloride, chloroquine and bafilamicin A1 were used to inhibit acidification of endosomes and clathrin-associated endocytosis. Nystatin was used as an inhibitor of the caveolae pathway. Cytochalasin D and ML-7 were used to inhibit actin and myosin functions, respectively. Nocodazole and colchicine were employed to inhibit microtubule activity. Virus entry was assessed by measuring viral transcription using real-time PCR, synthesis of capsid protein and assembly of infectious progeny virus in the presence of inhibitor blockage. The results indicated that BPV entry into embryonic bovine trachael cells utilizes endocytosis in clathrin-coated vesicles, is dependent upon acidification, and appears to be associated with actin and microtubule dependency. Evidence for viral entry through caveolae was not obtained. These findings provide a fuller understanding of the early cell-entry events of the replication cycle for members of the genus Bocavirus.

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“…Since inhibition of intracellular viral disassembly by NH 4 Cl, bafilomycin A or E64, or that of viral RNA synthesis by ribavirin should not prevent the extracellular attachment or internalization of the virus (Basak & Turner, 1992;Donelli et al, 1992;Greber et al, 1993), our data suggested that viral attachment and viral internalization might not be sufficient to induce chIL-1b mRNA expression because the early or late chIL-1b mRNA expression was abolished when viral disassembly or viral RNA synthesis were blocked, respectively. The possibility that receptor engagement by ARV might play a role in the induction of chIL-1b mRNA expression could not be excluded.…”

Section: Discussionmentioning

confidence: 59%

Characterization of interleukin-1β mRNA expression in chicken macrophages in response to avian reovirus

Liu

Shien

et al. 2008

Journal of General Virology

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Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1b (IL-1b) mRNA in chicken (chIL-1b) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1b mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1b mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1b mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1b mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA + virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response. INTRODUCTIONAvian reovirus (ARV) has a genome consisting of 10 segments of double-stranded RNA (dsRNA), which is encapsidated by a double-shell capsid (Spandidos & Graham, 1976). The virus encodes at least 13 primary translation products; eight of them are structural components and the other five are non-structural proteins (Varela & Benavente, 1994;Bodelon et al., 2001;Shmulevitz et al., 2002;Touris-Otero et al., 2004). Viruses have been isolated frequently from the gastrointestinal and respiratory tracts of chickens with a variety of disease conditions (Rosenberger, 2003). Among them, viral arthritis and pale bird syndrome are the most commonly recognized diseases in poultry.It has been demonstrated that the expression and release of pro-inflammatory cytokines, such as interleukin-1 (IL-1) and type I interferons, is a primary antiviral response in virus-infected cells (Jacobs & Langland, 1996;Iordanov et al., 2001;Maggi et al., 2003). In mammals, IL-1, which exhibits a broad spectrum of activities, has been extensively studied. It is a low molecular mass protein produced by many different cell types, but with stimulated macrophages being the main producers (Dinarello, 2000). IL-1 is an important factor in the pathogenesis of many diseases and in the mediation of a host response to infections through inflammatory and immunological events (Dinarello, 2000). IL-1b is a member of the IL-1 family (Burger et al., 2006). The primary translation product of the IL-1b gene is converted into a mature protein of approximately 17 kDa by the IL-1b converting enzyme, a proteinase that belongs to the caspase family of proteases (Nicholson & Thomberry, 1997). It is not glycosylated in spite of a number of potential N-glycosylation sites being present in it...

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Infectious entry pathway for canine parvovirus

Cited by 57 publications

References 28 publications

In Vivo, Dendritic Cells Can Cross-Present Virus-Like Particles Using an Endosome-to-Cytosol Pathway

In Vivo, Dendritic Cells Can Cross-Present Virus-Like Particles Using an Endosome-to-Cytosol Pathway

Bovine parvovirus uses clathrin-mediated endocytosis for cell entry

Characterization of interleukin-1β mRNA expression in chicken macrophages in response to avian reovirus

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