Infectious defective interfering particles of VSV from transcripts of a cDNA clone

Pattnaik, Asit K.; Ball, L. Andrew; LeGrone, Alison W.; Wertz, Gail W.

doi:10.1016/0092-8674(92)90619-n

Cited by 247 publications

(264 citation statements)

References 19 publications

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“…Two and three extra residues were indeed deleterious for the replication of the CAT (chloramphenicol acetyltransferase)-expressing minigenomes of human parainfluenza virus type 3 (De & Banerjee 1993) and measles virus (Radecke et al 1995), respectively. In contrast, similar SeV minigenome and VSV DI RNA were tolerant of up to two and four extra residues, respectively (Harty & Palese 1995;Pattnaik et al 1992). …”

Section: Discussionmentioning

confidence: 99%

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Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense

et al. 1996

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Background: The mononegavirus superfamily (Mononegavirales) comprises three families, Rhabdoviridae, Paramyxoviridae and Filoviridae. These viruses possess a single stranded negative sense RNA as the genome. Recentsuccessintherecoveryofinfectiousvirusfroma transfected cDNA of mononegaviruses including Sendai virus, a prototypic paramyxovirus, is opening the possibility of their genetic engineering. However, infectious viruses have been recovered only by initiating the infectious cycle with cDNA directing the synthesis of antigenomic positive sense ( ) RNA. Starting with genomic negative sense (ÿ) RNA has been unsuccessful. Furthermore, the recovery efficiency has often been extremely low.

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Section: Discussionmentioning

confidence: 99%

“…However, the extra nucleotides of the VSV DI RNA were removed during replication, yielding the authentic DI genome (Pattnaik et al 1992). As discussed above, essentially the same correction appeared to be necessary for recovering fully infectious SeV (Garcin et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense

et al. 1996

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“…To find conditions suitable for the rescue of infectious virus from a genome cDNA, a synthetic MUV minireplicon similar to those described for influenza virus and members of the Paramyxoviridae and Rhabdoviridae was assembled so as to contain the CAT reporter gene (5,7,8,20,24,28,35). Initially CAT activity was rescued by transfection of MUV-infected 293 cells with RNA transcribed from this construct in vitro.…”

Section: Discussionmentioning

confidence: 99%

Rescue of Mumps Virus from cDNA

Clarke

Sidhu

Johnson

et al. 2000

J Virol

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A complete DNA copy of the genome of a Jeryl Lynn strain of mumps virus (15,384 nucleotides) was assembled from cDNA fragments such that an exact antigenome RNA could be generated following transcription by T7 RNA polymerase and cleavage by hepatitis delta virus ribozyme. The plasmid containing the genome sequence, together with support plasmids which express mumps virus NP, P, and L proteins under control of the T7 RNA polymerase promoter, were transfected into A549 cells previously infected with recombinant vaccinia virus (MVA-T7) that expressed T7 RNA polymerase. Rescue of infectious virus from the genome cDNA was demonstrated by amplification of mumps virus from transfected-cell cultures and by subsequent consensus sequencing of reverse transcription-PCR products generated from infected-cell RNA to verify the presence of specific nucleotide tags introduced into the genome cDNA clone. The only coding change (position 8502, A to G) in the cDNA clone relative to the consensus sequence of the Jeryl Lynn plaque isolate from which it was derived, resulting in a lysine-to-arginine substitution at amino acid 22 of the L protein, did not prevent rescue of mumps virus, even though an amino acid alignment for the L proteins of paramyxoviruses indicates that lysine is highly conserved at that position. This system may provide the basis of a safe and effective virus vector for the in vivo expression of immunologically and biologically active proteins, peptides, and RNAs.

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“…This was first successfully used for intracellular generation of functional nodavirus RNA (Ball, 1992) and then of an artificial VSV DI RNA in Wertz's laboratory (Pattnaik et al, 1992). Virus cDNA derived from the natural 2 kb VSV DI-T was cloned between the T7 promoter and ribozyme sequences derived from either the genomic strand of tobacco ringspot virus (Prody et al, 1986) or the antigenomic strand of hepatitis delta virus (HDV; Perrotta & Been, 1990 and followed by a T7 polymerase transcription termination sequence.…”

Section: Production Of Rnps Entirely From Cdnaencoded Componentsmentioning

confidence: 99%

Genetic manipulation of non-segmented negative-strand RNA viruses

Conzelmann

1996

Journal of General Virology

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Infectious defective interfering particles of VSV from transcripts of a cDNA clone

Cited by 247 publications

References 19 publications

Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense

Initiation of Sendai virus multiplication from transfected cDNA or RNA with negative or positive sense

Rescue of Mumps Virus from cDNA

Genetic manipulation of non-segmented negative-strand RNA viruses

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