1990
DOI: 10.1099/0022-1317-71-11-2503
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Infectious cucumber mosaic virus RNA transcribed in vitro from clones obtained from cDNA amplified using the polymerase chain reaction

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Cited by 45 publications
(26 citation statements)
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References 42 publications
(38 reference statements)
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“…The lower numbers are amino acid residues in the la protein. (c) Relevant restriction endonuclease sites in the 2a coding region (boxed) and 3' flanking region of pCMV2A (Hayes et al, 1990b). The upper numbers are nucleotide residues corresponding to CMV RNA 2 (Rezaian et al, 1984).…”
Section: Introductionmentioning
confidence: 99%
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“…The lower numbers are amino acid residues in the la protein. (c) Relevant restriction endonuclease sites in the 2a coding region (boxed) and 3' flanking region of pCMV2A (Hayes et al, 1990b). The upper numbers are nucleotide residues corresponding to CMV RNA 2 (Rezaian et al, 1984).…”
Section: Introductionmentioning
confidence: 99%
“…1 b), a full-length cDNA clone of CMV RNA 1 (Hayes & Buck, 1990b), and two oligonucleotide primers, one of which contained an added EcoRI site and the other a BamHI site at the 5' end. After purification with a PrimerErase column (Stratagene), the PCR product was cleaved with EcoRI and BamHI and cloned into the corresponding sites of pMAL-cRI to give pMALla-A, pCMV1A was then cleaved with StuI and BamHI and the 3"2 kb fragment was cloned into the corresponding sites of pMALla-A to give pMALla-1.…”
Section: Introductionmentioning
confidence: 99%
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“…The CMV genome consists of the tripartite component RNAs (RNAs 1−3), and encodes five proteins (Palukaitis and Garcia-Arenal, 2003). RNA1 and RNA2 encode the 1a and 2a proteins, which are attributed viral replication (Hayes and Buck, 1990). RNA3 encodes the movement protein (3a) and coat protein (CP).…”
mentioning
confidence: 99%
“…In this paper we describe the use of reverse transcriptase-PCR reactions (RT-PCR) and a high fidelity Vent DNA polymerase (Mattila et al, 1991) for construction of fulllength cDNA clones of Ba, Mo, Suv and Tu strains of BBMV RNA components. Previously, Hayes & Buck (1990) have used a low fidelity Taq DNA polymerase for RT-PCR amplification of full-length CMV cDNAs. Transcribed BBMV RNAs have been used to determine the translational capacity of the individual BBMV segments and to demonstrate the repair of 5' termini in transcript-derived progeny BBMV RNAs.…”
mentioning
confidence: 99%