Infectious bursal disease virus of chickens: pathogenesis and immunosuppression

Sharma, J. M.; Kim, In Jeong; Rautenschlein, Silke; Yeh, Hung Yueh

doi:10.1016/s0145-305x(99)00074-9

Cited by 340 publications

(264 citation statements)

References 44 publications

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“…Infectious bursal disease (IBD), 3 also called Gumboro disease, is an acute, highly contagious disease in young chickens that occurs across the world (1). Its causative agent, IBD virus (IBDV), destroys its target cells, the B lymphocyte precursors (2)(3)(4).…”

Section: Infectious Bursal Disease (Ibd) Is An Acute Highly Contagiomentioning

confidence: 99%

“…Its causative agent, IBD virus (IBDV), destroys its target cells, the B lymphocyte precursors (2)(3)(4). IBDV infection may cause mortality in naïve chickens and very high mortality in chickens with low levels of neutralizing antibodies or no mortality at all but a high degree of immunosuppression (5).…”

Section: Infectious Bursal Disease (Ibd) Is An Acute Highly Contagiomentioning

confidence: 99%

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The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

Lin

Zhang

et al. 2015

Journal of Biological Chemistry

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Background: IBDV VP5 protein induces apoptosis in DF-1 cells via interaction with VDAC2. Results: RACK1 inhibits IBDV-induced apoptosis, enhances viral replication, and interacts with both VDAC2 and VP5. Conclusion: RACK1 forms a complex with VDAC2 and VP5, affecting IBDV-induced apoptosis and viral replication. Significance: RACK1 inhibits apoptosis via interaction with VDAC2, indicating sequestration of RACK1 and VDAC2 by VP5 during IBDV infection.

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Section: Infectious Bursal Disease (Ibd) Is An Acute Highly Contagiomentioning

confidence: 99%

Section: Infectious Bursal Disease (Ibd) Is An Acute Highly Contagiomentioning

confidence: 99%

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

Lin

Zhang

et al. 2015

Journal of Biological Chemistry

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“…The infection with IBDV may exacerbate previous infections with other infectious agents, and may reduce the capacity of the bird to respond to vaccination, as the virus damages the humoral and cellular immune responses of chickens (Sharma et al, 2000). Previous studies have demonstrated that IBDV strains with different virulence may differ in their ability to replicate in vivo, to induce humoral immunity, and to cause immunosuppression.…”

Section: Introductionmentioning

confidence: 99%

Infectious Bursal Disease: Pathogenicity and Immunogenicity of Vaccines

Camilotti

Moraes

Furian

et al. 2016

Rev. Bras. Cienc. Avic.

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The Infectious Bursal Disease (IBD) is a contagious viral disease that affects young chickens and may cause high morbidity and mortality. As the virus is very resistant to the environment, vaccination is required in case of high infection pressure. Due to variations in the virulence degree of the vaccines available to control IBD, this study aimed at evaluating the pathogenicity and immunogenicity of three types of vaccines. In total, 220 one-day-old specific pathogen free (SPF) chickens were immunized with recombinant, immune-complex and intermediate vaccines, or not vaccinated (55 birds per group) and challenged with IBD G11 strain on day 25. On days 25, 30, and 35, the Bursa of Fabricius (BF) were submitted to gross and histological examination, and serum samples were submitted to ELISA to determined anti-IBD antibody titers. On day 23, chickens were submitted to the test of hypersensitivity to phytohemagglutinin to evaluate the immunosuppressive effect of vaccines on the cell-mediated immunity. The results have indicated that the immune-complex vaccine induced the most severe BF lesions, whereas the recombinant vaccine preserved BF tissue and cell integrity. The three evaluated vaccines induced humoral immunity of similar intensity. The cellular reaction to phytohemagglutinin of the chickens immunized with recombinant and immune-complex vaccines was less severe compared with the unvaccinated chickens. In conclusion, these results indicate that the immune-complex vaccine was the most pathogenic and that all vaccines were effective in protecting SPF chickens against IBD.

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“…T cells may also promote the production of pro-inflammatory factors, such as nitric oxide, increasing tissue destruction. 18 Outbreaks with high mortality rates caused by very virulent IBDV (vvIBDV) strains have been reported in Europe, Asia, and Latin America. 21 Subclinical and immunosuppressive forms of the disease are prevalent in the United States, where reduction of body weight and lack of uniformity may be produced in mild outbreaks.…”

Section: Introductionmentioning

confidence: 99%

Detection of Infectious Bursal Disease virus from Formalin-Fixed Paraffin-Embedded Tissue by Immunohistochemistry and Real-Time Reverse Transcription-Polymerase Chain Reaction

2007

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Abstract. Formalin fixed paraffin embedded tissues blocks are used routinely to diagnose the economically important immunosuppressive infectious bursal disease virus (IBDV) in chickens. Immunohistochemical detection of viruses in tissue blocks has been done with varying results between laboratories. Extraction of IBDV RNA from tissue blocks allows IBDV strain identification at a molecular level. This allows correlation between virus identity and histological lesions present in the tissue. Experimentally reverse transcription-polymerase chain reaction (RT-PCR) detectable IBDV RNA could always be extracted from tissue blocks with acute +3 or higher histological lesion scores. However, many blocks from diagnostic field cases did not yield detectable IBDV RNA, in spite of having severe IBDV histological lesion scores. The reason for this can be the effect different formalin fixation conditions have on RNA detection from tissue blocks. To study the effect of various fixation parameters on RNA extraction and immunohistochemical detection of IBDV, bursas with maximum histological lesion score of 4 for IBDV were fixed in formalin under various conditions (different pH levels, temperatures, concentrations of formalin, and fixation duration). Only tissues fixed in formalin with a pH of 7.0, concentration of 5 or 10% formaldehyde, storage temperature of 25uC or less, and kept for up to 2 weeks in formalin yielded detectable IBDV RNA upon extraction. No RNA could be detected from tissues fixed under extreme temperature, pH, or formalin concentrations. Optimal fixation conditions for IHC detection of IBDV were 10% formalin concentration, pH 7.0, and temperature of 4uC, where maximum intensity of immunostaining was observed.

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Infectious bursal disease virus of chickens: pathogenesis and immunosuppression

Cited by 340 publications

References 44 publications

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

The Association of Receptor of Activated Protein Kinase C 1(RACK1) with Infectious Bursal Disease Virus Viral Protein VP5 and Voltage-dependent Anion Channel 2 (VDAC2) Inhibits Apoptosis and Enhances Viral Replication

Infectious Bursal Disease: Pathogenicity and Immunogenicity of Vaccines

Detection of Infectious Bursal Disease virus from Formalin-Fixed Paraffin-Embedded Tissue by Immunohistochemistry and Real-Time Reverse Transcription-Polymerase Chain Reaction

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