Infectious agents and inflammation in donated hearts and dilated cardiomyopathies related to cardiovascular diseases, Chagas' heart disease, primary and secondary dilated cardiomyopathies

Mangini, Sandrigo; Higuchi, Maria de Lourdes; Kawakami, Joyce T.; Reis, Marlene Paulino dos; Ikegami, Renata Nishiyama; Palomino, Suely Aparecida Pinheiro; Pomerantzeff, Pablo Maria Alberto; Fiorelli, Alfredo Inácio; Marcondes‐Braga, Fabiana G.; Bacal, Fernando; Ferreira, Sílvia Moreira Ayub; Issa, Victor Sarli; Souza, Germano Emílio Conceição; Chizzola, Paulo Roberto; Bocchi, Edimar Alcides

doi:10.1016/j.ijcard.2014.10.133

Cited by 16 publications

(12 citation statements)

References 41 publications

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“…Recently, Mangini et al detected the presence of Mycoplasma pneumoniae , Chlamydophila pneumoniae and Borrelia burgdorferi in hearts with dilated cardiomyopathy, including Chagas disease [20]. In the aortic valve, the proliferation of Chlamydophila pneumoniae and Mycoplasma pneumoniae was related to tissue calcification and aortic valve stenosis [21].…”

Section: Discussionmentioning

confidence: 99%

Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study

et al. 2017

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Background: The etiology of myxomatous mitral valve degeneration (MVD) is not fully understood and may depend on time or environmental factors for which the interaction of infectious agents has not been documented. The purpose of the study is to analyze the effect of Mycoplasma pneumoniae (Mp), Chlamydophila pneumoniae (Cp) and Borrelia burgdorferi (Bb) on myxomatous mitral valve degeneration pathogenesis and establish whether increased in inflammation and collagen degradation in myxomatous mitral valve degeneration etiopathogenesis. Methods: An immunohistochemical test was performed to detect the inflammatory cells (CD20, CD45, CD68) and Mp, Bb and MMP9 antigens in two groups. The in situ hybridization was performed to detect Chlamydophila pneumoniae and the bacteria study was performed using transmission electron microscopy. Group 1 (n = 20), surgical specimen composed by myxomatous mitral valve degeneration, and group 2 (n = 20), autopsy specimen composed by normal mitral valve. The data were analyzed using SigmaStat version 20 (SPSS Inc., Chicago, IL, USA). The groups were compared using Student's t test, Mann-Whitney test. A correlation analysis was performed using Spearman's correlation test. P values lower than 0.05 were considered statistically significant.

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Section: Discussionmentioning

confidence: 99%

Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study

et al. 2017

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“…Activation of inflammatory response and apoptosis in cardiomyocyte cells under pathological cardiac remodeling associated with cardiovascular diseases (CVDs) progression. Inflammation is now recognized with cardiac dysfunction, including myocardial infarction, cardiac hypertrophy, myocarditis, and heart failure [1,2]. Studies have revealed that many detrimental stimuli such as oxidized low-density lipoprotein (oxLDL), ischemia/reperfusion, high glucose, and lipopolysaccharide (LPS) can provoke apoptosis and damage to cardiomyocytes, which involve in the CVD pathogenesis [3,4].…”

Section: Introductionmentioning

confidence: 99%

Amnion membrane proteins attenuate LPS-induced inflammation and apoptosis by inhibiting TLR4/NF-κB pathway and repressing MicroRNA-155 in rat H9c2 cells

Akseh

Nemati

Zamani-Gharehchamani

et al. 2021

Immunopharmacology and Immunotoxicology

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Objective: Amnion membrane (AM) has been popular for the treatment of inflammatory disorders due to its cell repairing properties. This current study aims to find the underlying mechanisms of amnion membrane proteins (AMPs) against the pro-inflammatory miRNA, miR-155, miR-146, and antiapoptotic microRNA, miR-21, in LPS-treated H9c2 cells. Methods: Cell viability and apoptosis were determined by MTT assay and annexin V/PI staining. The production of the cytokines, TNF-a and IL-6 were evaluated by using qPCR and Enzyme-linked immunosorbent assay (ELISA), respectively. In addition, the expression of miRNAs was quantified by qPCR, and also the protein level of TLR4 and NF-kb was determined with western blotting. Results: We found that AMPs ameliorated LPS-induced reduction of cell viability and augment apoptosis in H9c2 cells. AMPs efficiently inhibited cytokine expression (IL-6 and TNF-a) and activity of TLR4/ NF-jB pathway in LPS-treated H9c2 cells. Correspondingly, in parallel with the suppression of proinflammatory cytokines and apoptosis, AMPs mitigated pro-inflammatory miRNA, miR-155 expression, while, the expression of miR-155 was found to be increased in LPS-treated H9c2 cells. Also, AMPs activated miR-146 expression in H9c2 cells under LPS treatment. Additionally, the elevated expression of miR-21 provoked by LPS was further enhanced by AMPs. Conclusions: In conclusion, AMPs could alleviate LPS-induced cardiomyocytes cells injury via up-regulation of miR-21, miR-146, and suppression of TLR4/NF-jB pathway, which plays a key role in the down-regulation of LPS-mediated miR-155 and inflammatory cytokine expression.

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“…In Chagas disease, one of the alterations that affect the electrocardiogram (ECG) signal is the abnormal variation of intervals such as the QT interval and morphology of the T wave expressed by their residuum [10]. The QT interval represents the ventricular depolarization and repolarization time interval.…”

Section: Introductionmentioning

confidence: 99%

ECG Multilead QT Interval Estimation Using Support Vector Machines

Cuadros

Dugarte

Wong

et al. 2019

Journal of Healthcare Engineering

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This work reports a multilead QT interval measurement algorithm for a high-resolution digital electrocardiograph. The software enables off-line ECG processing including QRS detection as well as an accurate multilead QT interval detection algorithm using support vector machines (SVMs). Two fiducial points (Qini and Tend) are estimated using the SVM algorithm on each incoming beat. This enables segmentation of the current beat for obtaining the P, QRS, and T waves. The QT interval is estimated by updating the QT interval on each lead, considering shifting techniques with respect to a valid beat template. The validation of the QT interval measurement algorithm is attained using the Physionet PTB diagnostic ECG database showing a percent error of 2.60±2.25 msec with respect to the database annotations. The usefulness of this software tool is also tested by considering the analysis of the ECG signals for a group of 60 patients acquired using our digital electrocardiograph. In this case, the validation is performed by comparing the estimated QT interval with respect to the estimation obtained using the Cardiosoft software providing a percent error of 2.49±1.99 msec.

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Infectious agents and inflammation in donated hearts and dilated cardiomyopathies related to cardiovascular diseases, Chagas' heart disease, primary and secondary dilated cardiomyopathies

Cited by 16 publications

References 41 publications

Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study

Infectious agents is a risk factor for myxomatous mitral valve degeneration: A case control study

Amnion membrane proteins attenuate LPS-induced inflammation and apoptosis by inhibiting TLR4/NF-κB pathway and repressing MicroRNA-155 in rat H9c2 cells

ECG Multilead QT Interval Estimation Using Support Vector Machines

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