Infections with the vascular pathogens Verticillium longisporum and Verticillium dahliae induce distinct disease symptoms and differentially affect drought stress tolerance of Arabidopsis thaliana

Reusche, Michael; Truskina, Jekaterina; Thole, Karin; Nagel, Leonhard; Rindfleisch, Sören; Tran, Van‐Tuan; Braus‐Stromeyer, Susanna A.; Braus, Gerhard H.; Teichmann, Thomas; Lipka, Volker

doi:10.1016/j.envexpbot.2013.12.009

Cited by 38 publications

(42 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, Reusche et al (2012) reported that at 7 dpi V. longisporum triggers de novo xylem formation that can compensate for compromised water transport in brassicaceous host plants, which can explain why this vascular pathogen does not affect drought stress responses in oilseed rape (Lopisso et al, 2017). Furthermore, the increased ABA levels in Arabidopsis might contribute to an increased drought stress tolerance that has been observed for V. longisporum-infected plants (Reusche et al, 2014).…”

Section: Discussionmentioning

confidence: 98%

Suppression of abscisic acid biosynthesis at the early infection stage of Verticillium longisporum in oilseed rape (Brassica napus)

Behrens

Schenke

Hossain

et al. 2019

Molecular Plant Pathology

View full text Add to dashboard Cite

Summary Verticillium longisporum infects oilseed rape (Brassica napus) and Arabidopsis thaliana. To investigate the early response of oilseed rape to the fungal infection, we determined transcriptomic changes in oilseed rape roots at 6 days post‐inoculation (dpi) by RNA‐Seq analysis, in which non‐infected roots served as a control. Strikingly, a subset of genes involved in abscisic acid (ABA) biosynthesis was found to be down‐regulated and the ABA level was accordingly attenuated in 6 dpi oilseed rape as compared with the control. Gene expression analysis revealed that this was mainly attributed to the suppression of BnNCED3‐mediated ABA biosynthesis, involving, for example, BnWRKY57. However, this down‐regulation of ABA biosynthesis could not be observed in infected Arabidopsis roots. Arabidopsis ABA‐ defective mutants nced3 and aao3 displayed pronounced tolerance to the fungal infection with delayed and impeded symptom development, even though fungal colonization was not affected in both mutants. These data suggest that ABA appears to be required for full susceptibility of Arabidopsis to the fungal infection. Furthermore, we found that in both 6 dpi oilseed rape and the Arabidopsis nced3 mutant, the salicylic acid (SA) signalling pathway was induced while the jasmonic acid (JA)/ethylene (ET) signalling pathway was concomitantly mitigated. Following these data, we conclude that in oilseed rape the V. longisporum infection triggers a host‐specific suppression of the NCED3‐mediated ABA biosynthesis, consequently increasing plant tolerance to the fungal infection. We believe that this might be part of the virulence strategy of V. longisporum to initiate/establish a long‐lasting compatible interaction with oilseed rape (coexistence), which appears to be different from the infection process in Arabidopsis.

show abstract

Section: Discussionmentioning

confidence: 98%

Suppression of abscisic acid biosynthesis at the early infection stage of Verticillium longisporum in oilseed rape (Brassica napus)

Behrens

Schenke

Hossain

et al. 2019

Molecular Plant Pathology

View full text Add to dashboard Cite

show abstract

“…Because of the long-term persistence of the pathogen in soil and its wide host range (including economically important crops such as cotton, tomato, potato, pepper, watermelon, mint and lettuce), it is difficult to control verticillium wilt (Bhat & Subbarao, 1999). Recently, an abundance of methods has been developed for the study of mechanisms involved in hostpathogen interactions, and more is now understood about the molecular basis of verticillium wilt disease and the molecular mechanisms by which V. dahliae infection causes disease (Fradin & Thomma, 2006;Maruthachalam et al, 2011;Xu et al, 2011;Santhanam & Thomma, 2013;Hoppenau et al, 2014;Reusche et al, 2014;Tian et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Disruption of Cerevisin via Agrobacterium tumefaciens‐mediated transformation affects microsclerotia formation and virulence of Verticillium dahliae

2015

Plant Pathology

View full text Add to dashboard Cite

Agrobacterium tumefaciens-mediated transformation (ATMT) was used to obtain a large number of Verticillium dahliae (Vd991) T-DNA insertion mutants that were randomly integrated. Insertion mutants that produced significantly fewer microsclerotia were chosen for further analysis. Mutant T0065 was identified as having the Cerevisin gene interrupted by T-DNA, and it was named cerevisin. The cerevisin protein showed a high amino acid sequence similarity with the vacuolar protease B. The mutant strain cerevisin displayed decreased production of microsclerotia and conidia, significantly reduced growth rate and reduction in virulence compared to the wild type. Moreover, the composition of secreted proteins differed between the cerevisin mutant and the wild type. Loss of function of Cerevisin decreased secretion of proteins of low molecular weight (14-25 kDa). Upon treatment with the secreted proteins of the mutant, the degree of leaf wilting decreased, indicating that Cerevisin is implicated in production of these proteins, which are putative pathogenicity factors of V. dahliae. The results suggest that Cerevisin is involved in controlling multiple processes of development and metabolism, plays an important role in vegetative growth and microsclerotia formation and affects virulence of V. dahliae.

show abstract

“…S6, see Supporting Information). These structures closely resembled those observed in planta (Vallad and Subbarao, ; Zhang et al ., ; Zhao et al ., ), which have been described recently as hyphopodia (Reusche et al ., ; Zhao et al ., ). Consistent with the differential behaviour in planta of Δ vhb1 and Δ vhp1 revealed by the re‐isolation from the vascular system assay and CLSM images, the former was able to penetrate cellophane paper, whereas the Δ vph1 strain was not (Fig.…”

Section: Resultsmentioning

confidence: 99%

Characterization of two homeodomain transcription factors with critical but distinct roles in virulence in the vascular pathogen Verticillium dahliae

Sarmiento-Villamil

Prieto

Klosterman

et al. 2017

Molecular Plant Pathology

View full text Add to dashboard Cite

Vascular wilt caused by Verticillium dahliae is a destructive disease that represents a chronic economic problem for crop production worldwide. In this work, we characterized two new regulators of pathogenicity in this species. Vph1 (VDAG_06555) was identified in a candidate gene approach as a putative homologue of the transcription factor Ste12. Vhb1 (VDAG_08786), identified in a forward genetics approach, is similar to the homeobox transcription factor Htf1, reported as a regulator of conidiogenesis in several fungi. Deletion of vph1 did not affect vegetative growth, whereas deletion of vhb1 greatly reduced sporulation rates in liquid medium. Both mutants failed to induce Verticillium wilt symptoms. However, unlike Δvph1, Δvhb1 could be re-isolated from the vascular system of some asymptomatic plants. Confocal microscopy further indicated that Δvph1 and Δvhb1 differed in their behaviour in planta; Δvph1 could not penetrate the root cortex, whereas Δvhb1 was impaired in its ability to colonize the xylem. In agreement with these observations, only Δvhb1 could penetrate cellophane paper. On cellophane, wild-type and Δvhb1 strains produced numerous short branches with swollen tips, resembling the hyphopodia formed on root surfaces, contrasting with Δvph1, which generated unbranched long filaments without swollen tips. A microarray analysis showed that these differences in growth were associated with differences in global transcription patterns, and allowed us to identify a large set of novel genes potentially involved in virulence in V. dahliae. Ste12 homologues are known regulators of invasive growth, but Vhb1 is the first putative Htf1 homologue identified with a critical role in virulence.

show abstract

Infections with the vascular pathogens Verticillium longisporum and Verticillium dahliae induce distinct disease symptoms and differentially affect drought stress tolerance of Arabidopsis thaliana

Cited by 38 publications

References 53 publications

Suppression of abscisic acid biosynthesis at the early infection stage of Verticillium longisporum in oilseed rape (Brassica napus)

Suppression of abscisic acid biosynthesis at the early infection stage of Verticillium longisporum in oilseed rape (Brassica napus)

Disruption of Cerevisin via Agrobacterium tumefaciens‐mediated transformation affects microsclerotia formation and virulence of Verticillium dahliae

Characterization of two homeodomain transcription factors with critical but distinct roles in virulence in the vascular pathogen Verticillium dahliae

Contact Info

Product

Resources

About