Infections with Centrocestus armatus Metacercariae in Fishes from Water Systems of Major Rivers in Republic of Korea

Abstract

The infection status of Centrocestus armatus metacercariae (CaMc) was broadly surveyed in freshwater fishes from major river systems in the Republic of Korea (Korea) during 2008–2017. A total of 14,977 fishes was caught and examined by the artificial digestion method. CaMc were detected in 3,818 (97.1%) (2,114 Z. platypus: 96.1% and 1,704 Z. temminckii: 98.4%) out of 3,932 Zacco spp. examined and their density was 1,867 (2,109 in Z. platypus and 1,567 in Z. temminckii) per fish infected. The prevalences with CaMc were high, 93.7–100%, in Zacco spp. from all surveyed areas. However, their densities were more or less different by the surveyed areas and fish species. They were most high in Nakdong-gang in Gyeongsangnam-do (4,201 in average), and followed by Geum-gang (2,343), Nakdong-gang in Gyeongsangbuk-do (1,623), Han-gang (1,564), Tamjin-gang and Yeongsan-gang (1,540), streams in the east coast (1,028), Seomjin-gang (488) and Mangyeong-gang (170). In another species of rasborinid fish, Opsariichthys uncirostris amurensis, CaMc were detected in 222 (74.8%) out of 297 ones examined and their density was 278 (1–4,480) per fish infected. CaMc were also detected in total 41 fish species except for the rasborinid fish, Z. platypus, Z. temminckii and O. uncirostris amurensis. Conclusively, it was confirmed that among the 3 species of rasborinid fish, Z. platypus and Z. temminckii are highly prevalent and O. uncirostris amurensis is moderately prevalent with CaMc. Additionally, we could know that variety of fish species act as the second intermediate hosts of C. armatus in Korea.

Infection Status with Digenetic Trematode Metacercariae in Fishes from Coastal Lakes in Gangwon-do, Republic of Korea

“…Fish species (No. of fish) examined were T. hakonensis (33), C. auratus (22), A. flavimanus (11), M. cephalus (11), T. brevispinis (10), A. lactipes (10), Gymnogobius castaneus (10), K. punctatus (5), Chelon haematocheilus (1).…”

Survey of Zoonotic Trematode Metacercariae in Fish from Water Systems of Geum-gang (River) in Republic of Korea

“…The chub fish species, Z. platypus , Z. koreanus and Z. temminckii , are known to be the susceptible fish hosts for C. armatus metacercariae (CaMc) in Korea [ 23 – 25 ]. In this study, the high endemicities with CaMc were shown in Z. platypus from Geum-gang (100% prevalence and over 3,300 CaMc intensity) in Geumsan-gun and Yugu-cheon (100% prevalence and over 7,000 CaMc intensity) in Gongju-si, Chungcheongnam-do.…”

“…In this study, the high endemicities with CaMc were shown in Z. platypus from Geum-gang (100% prevalence and over 3,300 CaMc intensity) in Geumsan-gun and Yugu-cheon (100% prevalence and over 7,000 CaMc intensity) in Gongju-si, Chungcheongnam-do. In a nation-wide survey of CaMc [ 25 ], the prevalences were very high, 93.7–100% (av. 97.1%), in chub fish species, Zacco spp., from all surveyed sites.…”

Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism

“…Fish bodies were dissected [38], gill arches were removed with the aid of a stereomicroscope (Leica, Milano, Italy) and prepared as wet mounts to be examined for the presence of encysted metacercariae [5,10,16,21,25,26,28,30,47]. Encysted metacercariae were examined under a light microscope (Leica, Milano, Italy) ( Figure 1B) to evaluate their morphology and to be identified according to published characteristics [5,[10][11][12][13][14][15][16][17]26]. Live encysted metacercariae were also recorded at 40× using a light microscope (Leica, Milano, Italy) (Supplementary Materials Video S1).…”

“…Within fish-borne trematodes, Centrocestus formosanus is a small heterophyid fluke, described for the first time in Taiwan [9] and widely distributed in Asia [4,5,[10][11][12][13][14][15]. Since the 1950s, several authors have reported this species in new continents, although its occurrence could still be underestimated [16][17][18][19][20][21][22].…”