Infection with Salmonella enterica Serovar Typhimurium Leads to Increased Proportions of F4/80+ Red Pulp Macrophages and Decreased Proportions of B and T Lymphocytes in the Spleen

Rosche, Kristin L.; Aljasham, Alanoud T.; Kipfer, James N.; Piatkowski, Bryan; Konjufca, Vjollca

doi:10.1371/journal.pone.0130092

Cited by 27 publications

(16 citation statements)

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“…It has been reported that S. typhimurium mainly attacks the intestine after it infects mice, which could induce the dysfunction of gastrointestinal tract and the damage of gastrointestinal mucous membrane, were consistented with our results [22], [23]. After infected with S. typhimurium, www.wjir.org most of mice have a systemic inflammatory response and an enlarged spleen [24]. Since S. typhimurium is a type of facultative intracellular pathogen, it could live in the macrophage [25], induce the apoptosis of macrophage, and release of mature proinflammatory cytokines, which could lead to the decrease of the macrophage ratio [26].…”

Section: Discussionsupporting

confidence: 92%

The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

Zhao¹,

Fotina²,

Wang³

et al. 2019

World Journal of Innovative Research

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Pages 33-39 33 www.wjir.org  Abstract-The infection of Salmonella is the main cause for gastrointestinal diseases worldwide. There are about 20 to 40 million cases with gastroenteritis caused by Salmonella infection across the world every year. In our study, we set up the Chinese Salmonella typhimurium CVCC541 infected mouse model by intraperitoneal injection with different amount of bacteria respectively. The results suggested the severity of clinical symptoms in mice was positively correlated with the dosage and duration of infection. Mice infected by S. typhimurium CVCC541 behaved loose coat and hunched back, sluggish, anorexic, and tendency to gather together. The necropsy results showed the severity of pathological changes was also positively correlated with the dosage of infection. The major lesion occurred in the intestine. Severe hemorrhage in the intestinal tract, especially in jejunum and ileum. Meanwhile, we found the amount of neutrophils increased, and the amounts of macrophages and T lymphocytes decreased significantly in the spleen of mice infected with S. typhimurium CVCC541. We first reported the clinical symptoms and pathological damages of mice caused by the infection of S. typhimurium CVCC541 at various concentrations in detail, which would provide the reference for studies of the molecular mechanism of Salmonella infection and drug resistance.

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Section: Discussionsupporting

confidence: 92%

The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

Zhao¹,

Fotina²,

Wang³

et al. 2019

World Journal of Innovative Research

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“…Inflammatory response plays an important role in the pathological damage such as liver fibrosis, intestinal inflammation, and splenomegaly during schistosomiasis. Macrophages, as one kind of important immunoregulatory cell, play an irreplaceable role in the process of inflammatory reaction as well as the pathological damage process of the spleen (27). Therefore, the in-depth study of macrophage-related inflammation is extremely important to clarify the pathogenesis of schistosomiasis characterized by splenomegaly.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-21 Mediates the Inhibiting Effect of Praziquantel on NLRP3 Inflammasome in Schistosoma japonicum Infection

Kong

Guo

et al. 2020

Front. Vet. Sci.

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Praziquantel (PZQ), a traditional helminthicide drug, has been shown to exert an anti-inflammatory effect on splenomegaly induced by schistosomiasis via regulating macrophage polarization. Meanwhile, miR-21 has been demonstrated to control macrophage polarization. However, the role of miR-21 in the regulation of macrophage polarization by PZQ in schistosomiasis is still unclear. In the present study, we found that M1-type macrophages were the predominant splenic macrophages in chronic schistosomiasis and that NLRP3 inflammasome-related molecules were upregulated. PZQ inhibited NLRP3 inflammasome in M1 macrophages and reduced the expression of miR-21. Furthermore, using the methods of quantitative real-time PCR and transfection, the downregulation of NLRP3/IL-1β by PZQ in M1 macrophages were reversed by miR-21 overexpression. These results indicated that miR-21 was involved in the inhibiting effect of PZQ on activation of NLRP3 inflammasome. Moreover, miR-21 might target Smad7 to mediate the anti-inflammatory effect of PZQ in polarized macrophages. This study provides an in-depth mechanism of PZQ in the treatment of schistosomiasis.

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“…However, based on our work, it would be profitable and interesting to examine the impact of brucellosis on other infectious and noninfectious diseases in the regions where it is common. It has been recently reported (21) that, like infection with Brucella, chronic infection of mice with Salmonella enterica serovar Typhimurium induces a significant decline in the proportions of CD169 ϩ MMMs, suggesting that MZ alteration by chronic bacterial infection could be a common occurrence.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies (17)(18)(19)(20)(21) investigating the impact of infection on MZ macrophage populations have performed only qualitative analyses by comparing their distribution by immunohistology. This approach does not allow for a fine comparison between several experimental conditions.…”

Section: Figmentioning

confidence: 99%

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Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen

et al. 2017

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The spleen is known as an important filter for blood-borne pathogens that are trapped by specialized macrophages in the marginal zone (MZ): the CD209 ϩ MZ macrophages (MZMs) and the CD169 ϩ marginal metallophilic macrophages (MMMs). Acute systemic infection strongly impacts MZ populations and the location of T and B lymphocytes. This phenomenon has been linked to reduced chemokine secretion by stromal cells. Brucella spp. are the causative agent of brucellosis, a widespread zoonotic disease. Here, we used Brucella melitensis infection as a model to investigate the impact of chronic stealth infection on splenic MZ macrophage populations. During the late phase of Brucella infection, we observed a loss of both MZMs and MMMs, with a durable disappearance of MZMs, leading to a reduction of the ability of the spleen to take up soluble antigens, beads, and unrelated bacteria. This effect appears to be selective as every other lymphoid and myeloid population analyzed increased during infection, which was also observed following Brucella abortus and Brucella suis infection. Comparison of wild-type and deficient mice suggested that MZ macrophage population loss is dependent on interferon gamma (IFN-␥) receptor but independent of T cells or tumor necrosis factor alpha receptor 1 (TNF-␣R1) signaling pathways and is not correlated to an alteration of CCL19, CCL21, and CXCL13 chemokine mRNA expression. Our results suggest that MZ macrophage populations are particularly sensitive to persistent low-level IFN-␥-mediated inflammation and that Brucella infection could reduce the ability of the spleen to perform certain MZM-and MMM-dependent tasks, such as antigen delivery to lymphocytes and control of systemic infection.KEYWORDS Brucella melitensis, marginal zone macrophages, low-grade Th1 inflammation, brucellosis, infection, spleen T he spleen is both the largest secondary lymphoid organ and the main filter of blood (for a general review, see reference 1). The white pulp, which contains T and B cell populations, is surrounded by the red pulp, a network of reticular fibers and fibroblasts rich in macrophages, where blood is filtered and old erythrocytes are removed. The marginal zone (MZ) located between the white pulp and the red pulp constitutes a specialized filtering area for blood content. The MZ contains specialized macrophage populations, various dendritic cell (DC) subsets, and MZ B cells. The marginal metallophilic macrophages (MMMs) form an inner ring between the MZ and white pulp, whereas MZ macrophages (MZMs) are situated at the outer boundary of the MZ,

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Infection with Salmonella enterica Serovar Typhimurium Leads to Increased Proportions of F4/80+ Red Pulp Macrophages and Decreased Proportions of B and T Lymphocytes in the Spleen

Cited by 27 publications

References 50 publications

The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

The Establishment of BALB/c Mouse Model Infected by Salmonella Typhimurium CVCC541

MicroRNA-21 Mediates the Inhibiting Effect of Praziquantel on NLRP3 Inflammasome in Schistosoma japonicum Infection

Chronic Brucella Infection Induces Selective and Persistent Interferon Gamma-Dependent Alterations of Marginal Zone Macrophages in the Spleen

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