Infection with Helicobacter pylori strains carrying babA2 and cagA is associated with an increased risk of peptic ulcer disease development in Iraq

Abdullah, Shahla M.; Hussein, Nawfal R.; Salih, Azad; Merza, Muayad Aghali; Goreal, Amer Abdalla; Odeesh, Odeesh Y.; Majed, Halat S.; Assafi, Mahde Saleh; Hawrami, Khidir

doi:10.1016/j.ajg.2012.12.001

Cited by 11 publications

(5 citation statements)

References 23 publications

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“…We looked within our populations for the associations between virulence factors and PUD. In agreement with previous reports from Iraq [19–21] , a significant association between cagA status and PUD was observed. Also, research from a neighbouring country, Turkey, has shown results similar to those from Iraq [3] .…”

Section: Discussionsupporting

confidence: 93%

Duodenal ulcer promoting gene 1 (dupA1) is associated with A2147G clarithromycin-resistance mutation but not interleukin-8 secretion from gastric mucosa in Iraqi patients

Hussein

Tunjel

Majed

et al. 2015

New Microbes and New Infections

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Helicobacter pylori causes peptic ulceration and gastric adenocarcinoma. The aims were to study the influence of dupA1 positivity upon interleukin-8 (IL-8) secretion from gastric mucosa and determine the prevalence of mutations responsible for clarithromycin and fluoroquinolone resistance. DNA was extracted from 74 biopsies and the virulence factors were studied. Levels of IL-8 in gastric mucosa were measured using ELISA and the mutations responsible for clarithromycin and fluoroquinolone resistance were determined using a GenoType-HelicoDR assay. The prevalence of cagA in strains isolated from gastric ulcer (GU) and duodenal ulcer (DU) was significantly higher than those isolated from non-ulcer disease (NUD) (90% and 57.9% versus 33.3%; p 0.01). The vacA s1m1 genotype was more prevalent in patients with DU (73.7%) and GU (70%) than in those with NUD (13.3%) (p 0.01). The prevalence of dupA1 was higher in DU patients (36.8%) than those with GU (10%) and NUD (8.9%) (p 0.01). Multivariate analysis showed that a cagA+/vacA s1i1m2 virulence gene combination was independently associated with the developing peptic ulcer disease (PUD) with increased odds of developing PUD (p 0.03; OR = 2.1). We found no significant difference in the levels of IL-8 secretion in gastric mucosa infected with H. pylori dupA-negative and H. pylori dupA1-positive strains (dupA-negative: mean ± median: 28 ± 26 versus 30 ± 27.1 for dupA1; p 0.6). While 12 strains were clarithromycin resistant, only three isolates were levofloxacin resistant. A significant association was found between dupA1 genotype and A2147G clarithromycin resistance mutation (p <0.01). Further study is needed to explore the relationship between virulence factors and disease process and treatment failure.

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Section: Discussionsupporting

confidence: 93%

Duodenal ulcer promoting gene 1 (dupA1) is associated with A2147G clarithromycin-resistance mutation but not interleukin-8 secretion from gastric mucosa in Iraqi patients

Hussein

Tunjel

Majed

et al. 2015

New Microbes and New Infections

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“…This fact highlights the importance of its detection in patients infected with H. pylori (Abdullah et al, 2012;Hatakeyama, 2004;Serrano et al, 2007). Of all H. pylori-positive patients analyzed in this study, 40.8% (64/157) were cagA positive, which is similar to the prevalence previously detected in Brazil (Leite et al, 2005;Ramis et al, 2010).…”

Section: Discussionsupporting

confidence: 81%

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Vianna

Ramis

Halicki

et al. 2015

Diagnostic Microbiology and Infectious Disease

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“…Many virulence-associated genes of H. pylori , including outer inflammatory protein a ( OipA ), vacuolating cytotoxin gene a ( vacA ), cytotoxin-associated gene a ( cagA ) and blood-group antigen-binding adhesion ( babA 2 ) are believed to have a critical role in determining the final clinical manifestation of the infection[9,10]. Therefore, various studies have conducted to discover better insights into the role of these proposed virulence factors in pathogenesis of digestive diseases[11-14]. None of the mentioned virulence factors have distinguished as discriminating factor in the development of peptic ulcer disease and gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

Role ofdupAin virulence ofHelicobacter pylori

Abadi¹,

Perez-Perez²

2016

WJG

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Helicobacter pylori (H. pylori) is a gastric human pathogen associated with acute and chronic gastritis, 70% of all gastric ulcers, 85% of all duodenal ulcers, and both forms of stomach cancer, mucosal-associated lymphoid tissue (MALT) lymphoma and adenocarcinoma. Recently, attention has focused on possible relationship between presence of certain virulence factor and H. pylori-associated diseases. Some contradictory data between this bacterium and related disorders has been observed since not all the colonized individuals develop to severe disease. The reported diseases plausibility related to H. pylori specific virulence factors became an interesting story about this organism. Although a number of putative virulence factors have been identified including cytotoxin-associated gene a (cagA) and vacA, there are conflicting data about their actual participation as specific risk factor for H. pylori-related diseases. Duodenal ulcer promoting gene a (dupA) is a virulence factor of H. pylori that is highly associated with duodenal ulcer development and reduced risk of gastric cancer. The prevalence of dupA in H. pylori strains isolated from western countries is relatively higher than in H. pylori strains from Asian countries. Current confusing epidemiological reports will continue unless future sophisticated and molecular studies provide data on functional and complete dupA cluster in H. pylori infected individuals. This paper elucidates available knowledge concerning role of dupA in virulence of H. pylori after a decade of its discovery.

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Infection with Helicobacter pylori strains carrying babA2 and cagA is associated with an increased risk of peptic ulcer disease development in Iraq

Cited by 11 publications

References 23 publications

Duodenal ulcer promoting gene 1 (dupA1) is associated with A2147G clarithromycin-resistance mutation but not interleukin-8 secretion from gastric mucosa in Iraqi patients

Duodenal ulcer promoting gene 1 (dupA1) is associated with A2147G clarithromycin-resistance mutation but not interleukin-8 secretion from gastric mucosa in Iraqi patients

Detection of Helicobacter pylori CagA EPIYA in gastric biopsy specimens and its relation to gastric diseases

Role ofdupAin virulence ofHelicobacter pylori

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