Infection-Related Declines in Chill Coma Recovery and Negative Geotaxis in Drosophila melanogaster

Linderman, Jessica A.; Chambers, Moria C.; Gupta, Avni S.

doi:10.1371/journal.pone.0041907

Cited by 42 publications

(38 citation statements)

References 46 publications

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“…Because fly phenotypes are influenced by bacteria [27], [28], [52], this bacterial variation can add to the variance of phenotypic traits. This makes it more difficult to detect genetic variation underlying phenotypic traits and reduces reproducibility between laboratories.…”

Section: Discussionmentioning

confidence: 99%

Host Species and Environmental Effects on Bacterial Communities Associated with Drosophila in the Laboratory and in the Natural Environment

et al. 2013

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The fruit fly Drosophila is a classic model organism to study adaptation as well as the relationship between genetic variation and phenotypes. Although associated bacterial communities might be important for many aspects of Drosophila biology, knowledge about their diversity, composition, and factors shaping them is limited. We used 454-based sequencing of a variable region of the bacterial 16S ribosomal RNA gene to characterize the bacterial communities associated with wild and laboratory Drosophila isolates. In order to specifically investigate effects of food source and host species on bacterial communities, we analyzed samples from wild Drosophila melanogaster and D. simulans collected from a variety of natural substrates, as well as from adults and larvae of nine laboratory-reared Drosophila species. We find no evidence for host species effects in lab-reared flies; instead, lab of origin and stochastic effects, which could influence studies of Drosophila phenotypes, are pronounced. In contrast, the natural Drosophila–associated microbiota appears to be predominantly shaped by food substrate with an additional but smaller effect of host species identity. We identify a core member of this natural microbiota that belongs to the genus Gluconobacter and is common to all wild-caught flies in this study, but absent from the laboratory. This makes it a strong candidate for being part of what could be a natural D. melanogaster and D. simulans core microbiome. Furthermore, we were able to identify candidate pathogens in natural fly isolates.

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Section: Discussionmentioning

confidence: 99%

Host Species and Environmental Effects on Bacterial Communities Associated with Drosophila in the Laboratory and in the Natural Environment

et al. 2013

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“…Drosophila immunity studies are somewhat divided between the two inoculation sites. In some studies pathogens are injected into the abdomen (24,26,(36)(37)(38)(39), and in others they are injected into the thorax (16,(40)(41)(42)(43), while many published papers do not report the site of infection at all. This procedural variance illustrates the general belief that site of inoculation is relatively unimportant to output phenotypes-a belief that is strongly contradicted by our current data.…”

Section: Discussionmentioning

confidence: 99%

Thorax Injury Lowers Resistance to Infection in Drosophila melanogaster

et al. 2014

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The route of infection can profoundly affect both the progression and outcome of disease. We investigated differences in Drosophila melanogaster defense against infection after bacterial inoculation into two sites-the abdomen and the thorax. Thorax inoculation results in increased bacterial proliferation and causes high mortality within the first few days of infection. In contrast, abdomen inoculation results in minimal mortality and lower bacterial loads than thorax inoculation. Inoculation into either site causes systemic infection. Differences in mortality and bacterial load are due to injury of the thorax and can be recapitulated by abdominal inoculation coupled with aseptic wounding of the thorax. This altered resistance appears to be independent of classical immune pathways and opens new avenues of research on the role of injury during defense against infection.

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“…Bacteria mediated D. melanogaster phenotypic effects range from pathogenic (2), over effects on cold tolerance (3), and effects on D. melanogaster nutritional status (4,5) to highly beneficial effects. For example, certain acetic acid bacteria (Acetobacteraceae) and Lactobacillaceae significantly promote D. melanogaster larval growth on amino acid poor diet (6,7) and can ensure longtime fertility and longevity under nutrient poor conditions (8).…”

Section: Introductionmentioning

confidence: 99%

Individual variation of natural D. melanogaster associated bacterial communities

Wang

Staubach

2017

Preprint

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D. melanogaster has become an important model organism to study host-microbe interaction.However, we still know little about the natural microbial communities that are associated with D.melanogaster. Especially, information on inter-individual variation is still lacking because most studies so far have used pooled material from several flies. Here, we collected bacterial 16S rRNA gene community profiles from a set of 32 individuals from a single population and compare the variation to that of samples collected from different substrates and locations. While community differences were on average larger between samples collected from different substrates, there was still a surprising amount of variation of microbial communities between individual flies. The samples clustered into two groups suggesting that there are yet unknown factors that affect the composition of natural fly associated microbial communities and need research. Importance D. melanogaster is an important model organism in evolutionary biology and also for the study of host-microbe interaction. In order to connect these to aspects of D. melanogaster biology, it is crucial to better understand the natural D. melanogaster microbiota because only the natural microbiota can affect the evolution of the host. We present, to our knowledge, the first data set that captures inter-individual variation of D. melanogaster associated bacterial communities.Clustering of communities into two larger groups suggests that there are important drivers of these communities that we do not understand yet suggesting in return that more research on the natural microbiota of D. melanogaster is needed.

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Infection-Related Declines in Chill Coma Recovery and Negative Geotaxis in Drosophila melanogaster

Cited by 42 publications

References 46 publications

Host Species and Environmental Effects on Bacterial Communities Associated with Drosophila in the Laboratory and in the Natural Environment

Host Species and Environmental Effects on Bacterial Communities Associated with Drosophila in the Laboratory and in the Natural Environment

Thorax Injury Lowers Resistance to Infection in Drosophila melanogaster

Individual variation of natural D. melanogaster associated bacterial communities

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