Infection pattern and transmission potential of chikungunya virus in two New World laboratory-adapted Aedes aegypti strains

Dong, Shao-Jing; Kantor, Asher M.; Lin, Jingyi; Passarelli, A. Lorena; Clem, Rollie J.; Franz, Alexander W. E.

doi:10.1038/srep24729

Cited by 42 publications

(98 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, midgut infection and dissemination of arboviruses are associated with the ingestion of a bloodmeal. CHIKV dissemination from the midgut has been observed as early as 24 h postoral acquisition of the virus, a time point at which the epithelial tissue of the blood-filled midgut was still overstretched (Dong et al, 2016). Thus, it appears that the virus was already disseminating from the midgut before transcriptional upregulation of Aemmp1 had reached its peak, which occurred at around 72 hpbm, a time point at which the bloodmeal was already digested.…”

Section: Discussionmentioning

confidence: 99%

Identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, Aedes aegypti

Kantor

Dong

Held

et al. 2016

Insect Molecular Biology

View full text Add to dashboard Cite

Aedes aegypti is a major vector for arboviruses such as dengue, chikungunya, and Zika viruses. During acquisition of a viremic bloodmeal, an arbovirus infects mosquito midgut cells before disseminating to secondary tissues, including the salivary glands. Once virus is released into the salivary ducts it can be transmitted to another vertebrate host. The midgut is surrounded by a basal lamina (BL) in the extracellular matrix, consisting of a proteinaceous mesh composed of collagen IV and laminin. BL pore size exclusion limit prevents virions from passing through. Thus, the BL likely requires remodelling via enzymatic activity to enable efficient virus dissemination. Matrix metalloproteinases (MMPs) are extracellular endopeptidases that are involved in remodelling of the extracellular matrix. Here, we describe and characterize the nine Ae. aegypti encoded MMPs, AeMMPs 1-9, which share common features with other invertebrate and vertebrate MMPs. Expression profiling in Ae. aegypti revealed that Aemmp4 and Aemmp6 were upregulated during metamorphosis, whereas expression of Aemmp1 and Aemmp2 increased during bloodmeal digestion. Aemmp1 expression was also upregulated in the presence of a bloodmeal containing chikungunya virus. Using polyclonal antibodies, AeMMP1 and AeMMP2 were specifically detected in tissues associated with the mosquito midgut.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, Aedes aegypti

Kantor

Dong

Held

et al. 2016

Insect Molecular Biology

View full text Add to dashboard Cite

show abstract

“…CHIKV prevalence into saliva of the HWE increases from 20% at 2 dpi to 60% at 7 dpi, differing from the ORL strain (55% at 2 dpi to 65% at 7 dpi)[31]. Our study also demonstrates that the increase of the ZIKV BR saliva detection rate was more pronounced in the HWE infected mosquitoes (0% at 7 dpi to 50% at 14 dpi) in relation to the RED (0% at 7 dpi to 11.1% at 14 dpi) and ROCK (11.1% at 7 dpi to 22.2% at 14 dpi).…”

Section: Discussionmentioning

confidence: 99%

Laboratory strains of Aedes aegypti are Competent to Brazilian Zika virus

Costa-da-Silva

Ioshino

Araújo

et al. 2016

Preprint

View full text Add to dashboard Cite

The Zika virus outbreaks are unprecedented human threat in relation to congenital malformations and neurological/autoimmune complications. Since this virus has high potential to spread in regions presenting the vectors, improvement in mosquito control is a top priority. Thus, Aedes aegypti laboratory strains will be fundamental to support studies in different research fields implicated on Zika-mosquito interactions which are the basis for the development of innovative control methods. In this sense, our aim was to determine the main infection aspects of a Brazilian Zika strain in reference Aedes aegypti laboratory mosquitoes. We orally exposed Rockefeller, Higgs and Rexville mosquitoes to the Brazilian ZIKV (ZIKV BR ) and qRT-PCR was applied to determine the infection, dissemination and detection rates of ZIKV in the collected saliva as well as viral levels in mosquito tissues. The three strains sustain the virus development but Higgs showed significantly lower viral loads in bodies at 14 days post-infection (dpi) and the lowest prevalences in bodies and heads. The Rockefeller strain was the most susceptible at 7 dpi but similar dissemination rates were observed at 14 dpi. Although variations exist, the ZIKV BR RNA shows detectable levels in saliva of the three strains at 14 dpi but is only detected in Rockefeller at 7 dpi. Moreover, saliva samples from the three strains were confirmed to be infectious when intrathoracically injected into mosquitoes. The ZIKV BR kinetics was monitored in Rockefeller mosquitoes and virus could be identified in the heads at 4 dpi but was more consistently detected late in infection. Our study presents the first evaluation on how Brazilian Zika virus behaves in reference Aedes aegypti strains and shed light on how the infection evolves over time. Vector competence and hallmarks of the ZIKV BR development were revealed in laboratory mosquitoes, providing additional information to accelerate studies focused on ZIKV-mosquito interactions.

show abstract

“…The presence of p400 in the mosquito tracheal system could indicate that it is involved in tracheal morphogenesis. As arboviruses can infect tracheal cells in A. aegypti (66), p400 could also mediate antiviral protection in these cells. Consistent with its role in hemocyte proliferation and differentiation in the fly model (51), p400 is expressed in hemocytes in A. aegypti females, with the protein localized in the nucleus of undifferentiated prohemocytes and in the nucleus and cytoplasm of differentiated hemocytes.…”

Section: Figmentioning

confidence: 99%

The Aedes aegypti Domino Ortholog p400 Regulates Antiviral Exogenous Small Interfering RNA Pathway Activity and ago-2 Expression

McFarlane

Almire

Kean

et al. 2020

mSphere

View full text Add to dashboard Cite

Arboviruses are pathogens of humans and animals. A better understanding of the interactions between these pathogens and the arthropod vectors, such as mosquitoes, that transmit them is necessary to develop novel control measures. A major antiviral pathway in the mosquito vector is the exogenous small interfering RNA (exo-siRNA) pathway, which is induced by arbovirus-derived double-stranded RNA in infected cells. Although recent work has shown the key role played by Argonaute-2 (Ago-2) and Dicer-2 (Dcr-2) in this pathway, the regulatory mechanisms that govern these pathways have not been studied in mosquitoes. Here, we show that the Domino ortholog p400 has antiviral activity against the alphavirus Semliki Forest virus (Togaviridae) both in Aedes aegypti-derived cells and in vivo. Antiviral activity of p400 was also demonstrated against chikungunya virus (Togaviridae) and Bunyamwera virus (Peribunyaviridae) but not Zika virus (Flaviviridae). p400 was found to be expressed across mosquito tissues and regulated ago-2 but not dcr-2 transcript levels in A. aegypti mosquitoes. These findings provide novel insights into the regulation of an important aedine exo-siRNA pathway effector protein, Ago-2, by the Domino ortholog p400. They add functional insights to previous observations of this protein’s antiviral and RNA interference regulatory activities in Drosophila melanogaster. IMPORTANCE Female Aedes aegypti mosquitoes are vectors of human-infecting arthropod-borne viruses (arboviruses). In recent decades, the incidence of arthropod-borne viral infections has grown dramatically. Vector competence is influenced by many factors, including the mosquito’s antiviral defenses. The exogenous small interfering RNA (siRNA) pathway is a major antiviral response restricting arboviruses in mosquitoes. While the roles of the effectors of this pathway, Argonaute-2 and Dicer-2 are well characterized, nothing is known about its regulation in mosquitoes. In this study, we demonstrate that A. aegypti p400, whose ortholog Domino in Drosophila melanogaster is a chromatin-remodeling ATPase member of the Tip60 complex, regulates siRNA pathway activity and controls ago-2 expression levels. In addition, we found p400 to have antiviral activity against different arboviruses. Therefore, our study provides new insights into the regulation of the antiviral response in A. aegypti mosquitoes.

show abstract

Infection pattern and transmission potential of chikungunya virus in two New World laboratory-adapted Aedes aegypti strains

Cited by 42 publications

References 56 publications

Identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, Aedes aegypti

Identification and initial characterization of matrix metalloproteinases in the yellow fever mosquito, Aedes aegypti

Laboratory strains of Aedes aegypti are Competent to Brazilian Zika virus

The Aedes aegypti Domino Ortholog p400 Regulates Antiviral Exogenous Small Interfering RNA Pathway Activity and ago-2 Expression

Contact Info

Product

Resources

About