Infection of the tracheal epithelium by infectious bronchitis virus is sialic acid dependent

Winter, Christine; Herrler, Georg; Neumann, Ulrich

doi:10.1016/j.micinf.2007.12.009

Cited by 57 publications

(66 citation statements)

References 21 publications

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“…QX strain is rapidly replicating virus causing complete ciliostasis of TOCs in the third day post infection comparable to the fifth day post infection for other relatively slowing replicating viruses as 4/91 and Beaudette strains (Lukert and Packer, 1972;Hu et al, 1975;Andrade et al, 1982;Winter et al, 2008;Abd El Rahman et al, 2009). In addition, TOCs can easily prepared, cultivated and since recent research is eager to establish alternative culture systems to reduce and/or replace infectious studies involving live animals.…”

Section: Discussionmentioning

confidence: 76%

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Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

El-Rahman¹,

Hoffmann²,

Lueschow³

et al. 2015

Adv. Anim. Vet. Sci.

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| Infectious bronchitis virus (IBV) is the causative agent of an acute and highly contagious disease affecting the respiratory, renal and/or reproductive systems of chicken called infectious bronchitis disease. Controlling of this disease needs continuous identification of the circulating strains and genotypes. In the present study, six IBV samples were isolated from different poultry farms located in northern Egypt and propagated in the allantoic cavity of specific pathogen-free embryonated chicken eggs causing curling and dwarfing of embryos at different serial egg passages. The harvested allantoic fluids were titrated by chicken embryo kidney cell cultures selecting suitable viral concentrations for investigating the ciliostasis effect of IBV on tracheal organ cell cultures. In addition, the nucleotide and protein sequences of the IBV spike glycoprotein (S) were used for molecular relationship analysing and construction of phylogenetic trees. All examined viruses induced complete ciliostasis at the fifth day post inoculation. Isolates shared total sequence identities of 99-100% on nucleotide and amino acid levels. The Egyptian strain (Eg/CLEVB-1/IBV/012) showed 99% and the Israeli strain (IS/1494/06 IBV) showed 98% with the collected isolates and recognized as the local strains with the highest total sequence identities and phylogenetic relatedness of all strains available from the Gene Bank. The Italy02 strain of IBV is the nearest relative to the collected isolates with total sequence identities of 79 and 77% at nucleotide and amino acid levels, respectively. The new variant strains currently isolated from the northern of Northern Egypt should be evaluated periodically for developing suitable autogenously based virus vaccines.

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Section: Discussionmentioning

confidence: 76%

“…TOCs were prepared according to Winter et al (2008) and incubated at 37°C under rotation. Only TOCs with a ciliary activity of 100% were selected for comparing of the ciliostatic effect of isolated and reference viruses.…”

Section: Inoculation Of Chicken Tracheal Organ Cell Cultures (Tocs)mentioning

confidence: 99%

Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

El-Rahman¹,

Hoffmann²,

Lueschow³

et al. 2015

Adv. Anim. Vet. Sci.

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“…The TOC were prepared from 20-day-old specific pathogen free chicken embryos (Lohmann, Cuxhaven, Germany) and 26-day-old turkey embryos (Moorgut Kartzfehn, Bösel, Germany) as previously described (Winter et al, 2008). Briefly, the tracheae were dissected and cleaned from connective tissue and attached muscles and cut with a sterile microtome blade into rings of approximately 1 mm thickness.…”

Section: Methodsmentioning

confidence: 99%

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Hartmann¹,

Sid²,

Rautenschlein³

2015

Avian Pathology

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Avian metapneumovirus (aMPV) is a pathogen with worldwide distribution, which can cause high economic losses in infected poultry. aMPV mainly causes infection of the upper respiratory tract in both chickens and turkeys, although turkeys seem to be more susceptible. Little is known about virus-host interactions at epithelial surfaces after aMPV infection. Tracheal organ cultures (TOC) are a suitable model to investigate virus-host interaction in the respiratory epithelium. Therefore, we investigated virus replication rates and lesion development in chicken and turkey TOC after infection with a virulent aMPV subtype A strain. Aspects of the innate immune response, such as interferon-α and inducible nitric oxide synthase mRNA expression, as well as virus-induced apoptosis were determined. The aMPV-replication rate was higher in turkey (TTOC) compared to chicken TOC (CTOC) (P < 0.05), providing circumstantial evidence that indeed turkeys may be more susceptible. The interferon-α response was down-regulated from 2 to 144 hours post infection in both species compared to virus-free controls (P < 0.05); this was more significant for CTOC than TTOC. Inducible nitric oxide synthase expression was significantly up-regulated in aMPV-A-infected TTOC and CTOC compared to virus-free controls (P < 0.05). However, the results suggest that NO may play a different role in aMPV pathogenesis between turkeys and chickens as indicated by differences in apoptosis rate and lesion development between species. Overall, our study reveals differences in innate immune response regulation and therefore may explain differences in aMPV -A replication rates between infected TTOC and CTOC, which subsequently lead to more severe clinical signs and a higher rate of secondary infections in turkeys.

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“…Tracheal organ cultures (TOCs) have previously been used for examining viruses, including IBV, within an in vitro environment (Raj & Jones, 1996;Wang et al, 1997;Winter et al, 2008). Such studies have allowed for the comparison of strain virulence, analysis of infection mechanisms and virus neutralization.…”

mentioning

confidence: 99%

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures

Ball

Bennett

Forrester

et al. 2016

Journal of General Virology

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Despite regular co-vaccination of two different strains of live infectious bronchitis vaccine viruses, little is known about possible mutations in these viruses following vaccination. As an alternative to chicks, this study used an in vitro infection model to identify single-nucleotide polymorphisms (SNPs) within the part-S1 gene of two live infectious bronchitis virus vaccine strains (793B and Massachusetts) following single or dual inoculation onto tracheal organ cultures. Results indicate that viral titres reduced over the duration of the study; conversely, the amount of detected infectious bronchitis virus genome increased. Results demonstrate a greater number of nonsynonymous SNPs in both vaccine strains when they are co-inoculated, compared with the single inoculations. The influence of the increased SNP and hydrophobic properties of the translated proteins on the vaccine viruses' virulence is unknown.

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Infection of the tracheal epithelium by infectious bronchitis virus is sialic acid dependent

Cited by 57 publications

References 21 publications

Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

Isolation and Characterization of New Variant Strains of Infectious Bronchitis Virus in Northern Egypt

Avian metapneumovirus infection of chicken and turkey tracheal organ cultures: comparison of virus–host interactions

Genetic mutations in live infectious bronchitis vaccine viruses following single or dual in vitro infection of tracheal organ cultures

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