Infection of the Cunner, Tautogolabrus adspersus (Walbaum), with Metacercariae of Cryptocotyle lingua (Creplin, 1825)

Sekhar, Sudarshan; Threlfall, William

doi:10.1017/s0022149x00021738

Cited by 7 publications

(5 citation statements)

References 19 publications

(12 reference statements)

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“…Potter, Gardner & Claridge (1988) and Shorter (1976) found that metacercarial cysts of C. lingua predominated above the lateral line of specimens of Merlangius merlangus. Sekhar & Threlfall (1970) found that infection by C. lingua was invariably lowest on the belly and the isthmus of Tautogolabrus adspersus. Van den Broek (1979) reported that among the gadoids Merlangius merlangus and Trisopterus luscus, C. lingua metacercariae were concentrated above the lateral line.…”

Section: Discussionmentioning

confidence: 96%

The effects of computer-controlled shadow stimuli on the success of cercarial transmission by Cryptocotyle lingua (Digenea: Heterophyidae)

Rea

Irwin

1992

Parasitology

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Pectoral fins from juvenile rainbow trout (Oncorhynchus mykiss) were used in a bioassay, the object of which was to quantify the effect of simulated shadow stimuli on the transmission success of the cercariae. For a period of 60 min, parasites and fins were exposed to a sequence of computer-controlled shadow stimuli, continuous light or total darkness, and subsequently the number of infections was counted. Trials were conducted with cercariae 1, 12 and 24 h old. Within the constraints of the experimental procedure, shadows were found to increase significantly the number of infections achieved by the 12-h-old parasites. As the cercariae aged, their ability to infect host tissue declined. The relevance of these results to transmission in the natural environment is discussed.

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Section: Discussionmentioning

confidence: 96%

The effects of computer-controlled shadow stimuli on the success of cercarial transmission by Cryptocotyle lingua (Digenea: Heterophyidae)

Rea

Irwin

1992

Parasitology

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“…Over the past few years, wild-caught cunners captured along the NL coastal waters by the field service team of the DOS, MUN, have often exhibited characteristic skin lesions and ulcers. Unfortunately, infectious diseases impacting cunner fish are not well characterized and documented [ 52 ]. In this study, we report the identification and complete characterization of a novel Gram-negative marine pathogen infecting wild cunners.…”

Section: Discussionmentioning

confidence: 99%

A Novel Marine Pathogen Isolated from Wild Cunners (Tautogolabrus adspersus): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380

et al. 2021

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Cunner (Tautogolabrus adspersus) is a cleaner fish being considered for utilized in the North Atlantic salmon (Salmo salar) aquaculture industry to biocontrol sea lice infestations. However, bacterial diseases due to natural infections in wild cunners have yet to be described. This study reports the isolation of Pseudomonas sp. J380 from infected wild cunners and its phenotypic, genomic, and transcriptomic characterization. This Gram-negative motile rod-shaped bacterium showed a mesophilic (4–28 °C) and halotolerant growth. Under iron-limited conditions, Pseudomonas sp. J380 produced pyoverdine-type fluorescent siderophore. Koch’s postulates were verified in wild cunners by intraperitoneally (i.p.) injecting Pseudomonas sp. J380 at 4 × 103, 4 × 105, and 4 × 107 colony forming units (CFU)/dose. Host-range and comparative virulence were also investigated in lumpfish and Atlantic salmon i.p. injected with ~106 CFU/dose. Lumpfish were more susceptible compared to cunners, and Atlantic salmon was resistant to Pseudomonas sp. J380 infection. Cunner tissues were heavily colonized by Pseudomonas sp. J380 compared to lumpfish and Atlantic salmon suggesting that it might be an opportunistic pathogen in cunners. The genome of Pseudomonas sp. J380 was 6.26 megabases (Mb) with a guanine-cytosine (GC) content of 59.7%. Biochemical profiles, as well as comparative and phylogenomic analyses, suggested that Pseudomonas sp. J380 belongs to the P. fluorescens species complex. Transcriptome profiling under iron-limited vs. iron-enriched conditions identified 1159 differentially expressed genes (DEGs). Cellular metabolic processes, such as ribosomal and energy production, and protein synthesis, were impeded by iron limitation. In contrast, genes involved in environmental adaptation mechanisms including two-component systems, histidine catabolism, and redox balance were transcriptionally up-regulated. Furthermore, iron limitation triggered the differential expression of genes encoding proteins associated with iron homeostasis. As the first report on a bacterial infection in cunners, the current study provides an overview of a new marine pathogen, Pseudomonas sp. J380.

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“…Host species reported infected with Cryptocotyle include Littorina littorea snails from Western Scotland (McQueen et al 1973) and the Baltic, North and Celtic seas (Europe) (Thieltges et al 2009), North American fish, i.e. herrings (Clupea harengus) from the northern New England coast (USA) (Sindermann & Farrin 1962) and cunners (Tautogolabrus adspersus) from the Newfoundland coast (Canada) (Sekhar 1970), as well as stray dogs from Rebun Island, Hokkaido (Japan) (Yoshimura 1965). Similarly, Cryptocotyle infections have been recorded in many North European marine fish species including Atlantic cod (Gadus morhua) (Borges et al 2015;Koie 1984) and whiting (Merlangius merlangus) (Rea & Irwin 1991).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel

et al. 2021

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Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting (Merlangius merlangus) and Atlantic cod (Gadus morhua) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems.

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Infection of the Cunner, Tautogolabrus adspersus (Walbaum), with Metacercariae of Cryptocotyle lingua (Creplin, 1825)

Cited by 7 publications

References 19 publications

The effects of computer-controlled shadow stimuli on the success of cercarial transmission by Cryptocotyle lingua (Digenea: Heterophyidae)

The effects of computer-controlled shadow stimuli on the success of cercarial transmission by Cryptocotyle lingua (Digenea: Heterophyidae)

A Novel Marine Pathogen Isolated from Wild Cunners (Tautogolabrus adspersus): Comparative Genomics and Transcriptome Profiling of Pseudomonas sp. Strain J380

Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel

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