Infection of specific strains of Streptococcus mutans, oral bacteria, confers a risk of ulcerative colitis

Kojima, Ayuchi; Nakano, Kazuhiko; Wada, Koichiro; Takahashi, Hirokazu; Katayama, Kazufumi; Yoneda, Masato; Higurashi, Takuma; Nomura, Ryota; Hokamura, Kazuya; Muranaka, Yoshinori; Matsuhashi, Nobuyuki; Umemura, Kazuo; Kamisaki, Yoshínori; Nakajima, Atsushi; Ooshima, Takashi

doi:10.1038/srep00332

Cited by 101 publications

(88 citation statements)

References 29 publications

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“…Though renal insufficiency is known to be a complication of IE, the relationship between S. mutans and kidney disease remains to be elucidated, and further studies should be performed. We previously reported that S. mutans might be a potential risk factor in aggravation of ulcerative colitis (58). In the present study, gastrointestinal hemorrhage and cystorrhagia were also observed.…”

Section: Figsupporting

confidence: 60%

Contribution of the Interaction of Streptococcus mutans Serotype k Strains with Fibrinogen to the Pathogenicity of Infective Endocarditis

et al. 2014

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dStreptococcus mutans, a pathogen responsible for dental caries, is occasionally isolated from the blood of patients with bacteremia and infective endocarditis (IE). Our previous study demonstrated that serotype k-specific bacterial DNA is frequently detected in S. mutans-positive heart valve specimens extirpated from IE patients. However, the reason for this frequent detection remains unknown. In the present study, we analyzed the virulence of IE from S. mutans strains, focusing on the characterization of serotype k strains, most of which are positive for the 120-kDa cell surface collagen-binding protein Cbm and negative for the 190-kDa protein antigen (PA) known as SpaP, P1, antigen I/II, and other designations. Fibrinogen-binding assays were performed with 85 clinical strains classified by Cbm and PA expression levels. The Cbm ؉ /PA ؊ group strains had significantly higher fibrinogen-binding rates than the other groups. Analysis of platelet aggregation revealed that SA31, a Cbm ؉ /PA ؊ strain, induced an increased level of aggregation in the presence of fibrinogen, while negligible aggregation was induced by the Cbmdefective isogenic mutant SA31CBD. A rat IE model with an artificial impairment of the aortic valve created using a catheter showed that extirpated heart valves in the SA31 group displayed a prominent vegetation mass not seen in those in the SA31CBD group. These findings could explain why Cbm ؉ /PA ؊ strains are highly virulent and are related to the development of IE, and the findings could also explain the frequent detection of serotype k DNA in S. mutans-positive heart valve clinical specimens.

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Section: Figsupporting

confidence: 60%

Contribution of the Interaction of Streptococcus mutans Serotype k Strains with Fibrinogen to the Pathogenicity of Infective Endocarditis

et al. 2014

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“…Avid binding of collagenous tissues is an important mechanism utilized by a subset of S. mutans strains expressing CBPs to successfully colonize oral and extraoral tissues (1,22,24,26,27,49). This study highlights two important characteristics that can be attributed to Cnm, (i) enhanced invasion of epithelial cell lines relevant to soft tissues of the oral cavity and (ii) stringent adherence to collagenous oral hard tissues, which enhance the colonization and cariogenic potentials of certain S. mutans strains.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier studies have demonstrated that streptococci dominate the intracellular flora of buccal cells in vivo (50) and have also linked Cnm expression in S. mutans with invasion of HCAEC (26) and HUVEC (27) and adherence to hepatocytes (49). These observations prompted our investigation into Cnm-mediated attachment to, and invasion of, oral epithelial cell lines.…”

Section: Discussionmentioning

confidence: 99%

The Collagen Binding Protein Cnm Contributes to Oral Colonization and Cariogenicity of Streptococcus mutans OMZ175

et al. 2015

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f Streptococcus mutans is the etiological agent of dental caries and one of the many bacterial species implicated in infective endocarditis. The expression of the collagen-binding protein Cnm by S. mutans has been associated with extraoral infections, but its relevance for dental caries has only been theorized to date. Due to the collagenous composition of dentinal and root tissues, we hypothesized that Cnm may facilitate the colonization of these surfaces, thereby enhancing the pathogenic potential of S. mutans in advancing carious lesions. As shown for extraoral endothelial cell lines, Cnm mediates the invasion of oral keratinocytes and fibroblasts by S. mutans. In this study, we show that in the Cnm ؉ native strain, OMZ175, Cnm mediates stringent adhesion to dentinal and root tissues as well as collagen-coated surfaces and promotes both cariogenicity and carriage in vivo. In vitro, ex vivo, and in vivo experiments revealed that while Cnm is not universally required for S. mutans cariogenicity, it contributes to (i) the invasion of the oral epithelium, (ii) enhanced binding on collagenous surfaces, (iii) implantation of oral biofilms, and (IV) the severity of caries due to a native Cnm ؉ isolate. Taken together, our findings reveal that Cnm is a colonization factor that contributes to the pathogenicity of certain S. mutans strains in their native habitat, the oral cavity.

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“…S. mutans has several major surface protein antigens, including collagen-binding protein (Cnm), a cell surface collagen-binding protein related to the ability to bind to the extracellular matrix, which has been purified and its encoding gene specified [15]. Our recent studies suggest that cnm -positive S. mutans strains could be associated with aggravated cerebral haemorrhaging [16-18], non-alcoholic steatohepatitis [19, 20] and inflammatory bowel disease [21]. We also demonstrated that cnm -positive S. mutans was associated with IgAN [22, 23].…”

Section: Introductionmentioning

confidence: 99%

<b><i>Campylobacter rectus</i></b> in the Oral Cavity Correlates with Proteinuria in Immunoglobulin A Nephropathy Patients

Misaki

Naka

Wato

et al. 2018

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Background: Periodontitis-related pathogens, such as Campylobacter or Treponema species, have recently been shown to be associated with immunoglobulin A nephropathy (IgAN). Some strains of Streptococcus mutans, a major pathogen of dental caries, harbour the cnm gene that encodes a collagen-binding protein (Cnm). This has also been demonstrated to be associated with urinary protein levels in IgAN patients. Objectives: The purpose of the present study was to analyse the association of IgAN with C. rectus, Treponema denticola and cnm-positive S. mutans in the oral cavity of humans. Methods: The presence of C. rectus, T. denticola and cnm-positive S. mutans strains in saliva samples of 117 IgAN patients and 56 healthy controls was evaluated by PCR, and the subjects’ clinical parameters were analysed. Results: C. rectus was significantly more prevalent in the IgAN group than in the control group (p < 0.05). The C. rectus-positive group was significantly associated with proteinuria in the IgAN group (p < 0.05). In addition, the C. rectus-positive and cnm-positive S. mutans group was shown to be more closely associated with urinary protein levels than the other groups (p < 0.0083). Conclusion: Our results suggest that harbouring C. rectus in the oral cavity could be associated with proteinuria in IgAN patients.

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Infection of specific strains of Streptococcus mutans, oral bacteria, confers a risk of ulcerative colitis

Cited by 101 publications

References 29 publications

Contribution of the Interaction of Streptococcus mutans Serotype k Strains with Fibrinogen to the Pathogenicity of Infective Endocarditis

Contribution of the Interaction of Streptococcus mutans Serotype k Strains with Fibrinogen to the Pathogenicity of Infective Endocarditis

The Collagen Binding Protein Cnm Contributes to Oral Colonization and Cariogenicity of Streptococcus mutans OMZ175

<b><i>Campylobacter rectus</i></b> in the Oral Cavity Correlates with Proteinuria in Immunoglobulin A Nephropathy Patients

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