Infection of silkmoth follicular cells with Bombyx mori nuclear polyhedrosis virus

Cytopathological process by multiple nucleopolyhedrovirus in the testis of Bombyx mori L., 1758 (Lepidoptera: Bombycidae)

“…periods that were analyzed may have been insufficient for detection of viral infection, since the BL may greatly delay infection in certain organs, as Smith-Johannsen et al (1986) have already commented. However, the experiment could not have been carried out in later periods p.i.…”

Development of a multiplex polymerase chain reaction for the simultaneous detection of microsporidians, nucleopolyhedrovirus, and densovirus infecting silkworms

“…The NPV infection was detected only from larval samples whereas, no detection was observed in pupa, moth and egg samples. There are conflicting reports of transovarial transmission of NPV (Johannsen et al, 1986;Khurad et al, 2004) and the PCR based detection of NPV warrants further study. Prior reports have described detection of NPV, microsporidian, and DNV by individual PCR methods (Abe et al, 1993;Noguchi et al, 1995;Kawakami et al, 1995).…”

Tissue-specific expression of silkmoth chorion genes in vivo using Bombyx mori nuclear polyhedrosis virus as a transducing vector.

“…This is due to the fact that viral infection of the follicular epithelium that surrounds each occyte is not uniform, even in the presence of collagenase (20); therefore, not all follicular cells of infected animals contain virally transduced chorion genes. The follicular epithelium that surrounds each follicle consists of approximately 5000 cells (27).…”

“…4, lane T), which revealed the presence of considerable quantities of transcripts of various lengths but complete absence of mature mRNA. The prominence of the hybridization signals obtained in the dot hybridizations of the nonfollicular RNA preparations is probably due to the fact that fat body, nervous tissue, muscle, and tissue culture cells, which are either covered by a relatively thin basal lamina or lack one completely, are infected by the virus more readily than follicular cells (20,28,29). Because of the presence of the thick basement membrane that completely surrounds each follicle, the latter are apparently infected by the recombinant virus only after a considerable lag: tissues such as fat body and carcass were found to contain large quantities of viral DNA 24 hr after inoculation with the virus, while viral DNA sequences could only be detected in follicular cells 36-48 hr after inoculation, and their abundance was significantly lower than that of the cells of other tissues (data not shown).…”