Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43

Bonavia, Aurelio; Arbour, Nathalie; Yong, V. Wee; Talbot, Pierre J.

doi:10.1128/jvi.71.1.800-806.1997

Cited by 82 publications

(89 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Up until now, no clear specific association has ever been made with any known human neuropathology. However, even though the mechanism by which they reach the human CNS is still to be decrypted, at least three of the six coronaviruses that infect humans were shown to be neuroinvasive and neurotropic in humans: HCoV-229E and HCoV-OC43 (Arbour et al, 1999a(Arbour et al, , 1999b(Arbour et al, , 2000Bonavia et al, 1997), as well as SARS-CoV (Gu et al, 2005;Xu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Human coronaviruses: Viral and cellular factors involved in neuroinvasiveness and neuropathogenesis

et al. 2014

View full text Add to dashboard Cite

Among the various respiratory viruses infecting human beings, coronaviruses are important pathogens, which usually infect the upper respiratory tract, where they are mainly associated with common colds. However, in more vulnerable populations, such as newborns, infants, the elderly and immune-compromised individuals, these opportunistic pathogens can also affect the lower respiratory tract, leading to pneumonia, exacerbations of asthma, and various types of respiratory distress syndrome. The respiratory involvement of human coronaviruses has been clearly established since the 1960s. Nevertheless, for almost three decades now, data reported in the scientific literature has also demonstrated that, like it was described for other human viruses, coronaviruses have neuroinvasive capacities since they can spread from the respiratory tract to the central nervous system (CNS). Once there, infection of CNS cells (neurotropism) could lead to human health problems, such as encephalitis and long-term neurological diseases. Neuroinvasive coronaviruses could damage the CNS as a result of misdirected host immune responses that could be associated with autoimmunity in susceptible individuals (virus-induced neuroimmunopathology) and/or viral replication, which directly induces damage to CNS cells (virus-induced neuropathology). Given all these properties, it has been suggested that these opportunistic human respiratory pathogens could be associated with the triggering or the exacerbation of neurologic diseases for which the etiology remains poorly understood. Herein, we present host and viral factors that participate in the regulation of the possible pathogenic processes associated with CNS infection by human coronaviruses and we try to decipher the intricate interplay between virus and host target cells in order to characterize their role in the virus life cycle as well as in the capacity of the cell to respond to viral invasion.

show abstract

Section: Introductionmentioning

confidence: 99%

Human coronaviruses: Viral and cellular factors involved in neuroinvasiveness and neuropathogenesis

et al. 2014

View full text Add to dashboard Cite

show abstract

“…These ubiquitous but not well studied viruses have been associated with 30% of common colds in humans (Myint, 1995). Experimentally, both the 229E and OC43 prototype strains can infect human astrocytes and microglia in primary cultures (Bonavia et al, 1997) and their RNA was detected in a significant proportion of human autopsy brain samples Arbour and Talbot, unpublished results), which is consistent with their neurotropic potential. In clinical studies, various reports have associated human coronaviruses with nosocomial respiratory infections in premature newborns, as diagnosed by indirect immunofluorescence (Sizun et al, 1995), and enterocolitis in neonates with evidence of human coronaviruses shown by electron microscopy (Chany et al, 1982).…”

Section: Introductionmentioning

confidence: 61%

“…The technique we used would be more appropriate for treating a large number of human samples containing few cells. We have already reported the use of the RT-PCR technique with clinical samples Bonavia et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

“…mixed with 80 vl of PBS, spun down at 5900× g for 10 min (Eppendorf centrifuge, Model 5415C) and resuspended in 150 vl of 0.1% (v/v) diethyl pyrocarbonate (DEPC; Sigma). The solution was boiled for 5 min, cellular debris eliminated by centrifugation for 2 min at 12000× g and the supernatant used immediately or stored at − 90°C (Bonavia et al, 1997). The oligonucleotide primer pairs used for RT-PCR are described in Table 1.…”

Section: Immunoperoxidase Assay For Infectious 6irusmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture

Sizun

Arbour

Talbot

1998

Journal of Virological Methods

View full text Add to dashboard Cite

Human coronaviruses, with two known serogroups named 229E and OC43, cause up to one third of common colds and may be associated with serious diseases such as nosocomial respiratory infections, enterocolitis, pericarditis and neurological disorders. Reliable methods of detection in clinical samples are needed for a better understanding of their role in pathology. As a first step in the design of such diagnostic procedures, the sensitivities and specificities of two viral diagnostic assays were compared in an experimental cell culture model: an indirect immuno-fluorescence assay using monoclonal antibodies and reverse transcriptase-polymerase chain reaction amplification of viral RNA from infected cells. Immunofluorescence detected human coronaviruses in cells infected at a MOI as low as 10(-2) (log TCID50/ml = 4.25 for HCV-229E and 2.0 for HCV-OC43; log PFU/ml = 4.83 for HCV-229E and 1.84 for HCV-OC43) versus 10(-3) (HCV-OC43) or 10(-4) (HCV-229E) for reverse transcriptase-polymerase chain reaction amplification (log TCID50/ml = 1.75 for HCV-229E and 1.5 for HCV-OC43; log PFU/ml = 2.3 for HCV-229E and 1.34 for HCV-OC43). There were no false positive signals with other human respiratory pathogens: influenza virus, respiratory syncytial virus and adenovirus. Moreover, each assay was coronavirus serogroup-specific. These results demonstrate the potential usefulness of immunofluorescence with monoclonal antibodies and reverse transcriptase-polymerase chain reaction RNA amplification for the rapid detection of human coronaviruses in infected cell cultures. Both methods could be applied to clinical specimens for the diagnosis of human infections.

show abstract

“…More specifically, human coronaviruses (HCoV) and virus-specific antibodies have been detected respectively in the brain and cerebrospinal fluids of MS patients (Burks et al, 1980;Murray et al, 1992;Stewart et al, 1992;Arbour et al, 2000;Salmi et al, 1982). Furthermore, acute infection of human microglia and astrocytes in primary cultures (Bonavia et al, 1997) and persistent infection of CNS cell lines (Arbour et al, 1999a,b) both demonstrate the neurotropism of human coronavirus.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profile within the mouse central nervous system and activation of myelin-reactive T cells following murine coronavirus infection

Gruslin

Moisan

St‐Pierre

et al. 2005

Journal of Neuroimmunology

View full text Add to dashboard Cite

Multiple sclerosis (MS) is an autoimmune disease associated with environmental factors, possibly including several viruses such as the coronaviruses. Indeed, murine coronavirus (MHV) infection provides a well-known experimental model for MS studies. Intracerebral infection of C57BL/6 mice with MHV-A59 revealed that viral replication was efficient and that clearance of infectious virus occurred as soon as 7 days post-infection. Using cDNA arrays, analysis of gene expression profile in the brain revealed a modulation of 80 different genes following infection, with at least 27 of these genes having previously been directly related to innate or acquired immune responses. Concordingly, an important activation of auto-reactive T cells specific to myelin basic protein was demonstrated. Altogether, these results indicate that an MHV infection of the central nervous system (CNS) leads to an important host genomic response implicating immunity-related genes and to the activation of myelin-reactive autoimmune T cells.

show abstract

Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43

Cited by 82 publications

References 25 publications

Human coronaviruses: Viral and cellular factors involved in neuroinvasiveness and neuropathogenesis

Human coronaviruses: Viral and cellular factors involved in neuroinvasiveness and neuropathogenesis

Comparison of immunofluorescence with monoclonal antibodies and RT-PCR for the detection of human coronaviruses 229E and OC43 in cell culture

Transcriptome profile within the mouse central nervous system and activation of myelin-reactive T cells following murine coronavirus infection

Contact Info

Product

Resources

About