Infection of Human Polyomaviruses JC and BK in Peripheral Blood Leukocytes from Immunocompetent Individuals

Dörries, Kristina; Vogel, Erika; Günther, Stefan; Czub, Stephanie

doi:10.1006/viro.1994.1008

Cited by 214 publications

(186 citation statements)

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“…Infected lymphocytes could then carry BKV to the bloodstream, allowing virus transport to other organs. The PCR technology disclosed BKV DNA and RNA in brain, peripheral blood mononuclear cells (Elsner and Do¨rries, 1992;De Mattei et al, 1994, 1995Do¨rries et al, 1994;Dolei et al, 2000), kidney, bladder, prostate, uterine cervix, vulva, lips and tongue (Monini et al, 1995a). The results obtained by PCR indicate that BKV can establish latent infection in many more organs and cells than previously thought.…”

Section: Characteristics Of Bkv Infection In Humansmentioning

confidence: 99%

Oncogenic transformation by BK virus and association with human tumors

et al. 2003

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BK virus (BKV), a human polyomavirus closely related to JC virus and Simian Virus 40, is ubiquitous in human populations worldwide. After primary infection, BKV establishes a lifelong latent infection in many organs. BKV transforms rodent cells to the neoplastic phenotype and is highly oncogenic in rodents. This review considers the oncogenic potential of BKV in humans and its possible involvement in human tumors. BKV sequences and T antigen (Tag) are detected in several types of human neoplasms, although the viral load is generally low, with less than one copy of the viral genome per cell. The possible causative role of BKV in human oncogenesis rests on the ability of BKV Tag to inactivate the functions of tumor suppressor proteins p53 and pRB family as well as on its ability to induce chromosomal aberrations in human cells. A 'hit and run' mechanism and secretion of paracrine growth factors by BKV Tag-positive cells, recruiting into proliferation neighboring and distant cells, are discussed as possible BKV pathogenic elements in human oncogenesis.

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Section: Characteristics Of Bkv Infection In Humansmentioning

confidence: 99%

Oncogenic transformation by BK virus and association with human tumors

et al. 2003

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“…Antibodies to BKV have been demonstrated in about 80% of normal adults (Dorries et al, 1994). Southern blots detected BKV DNA in 30% of cadaver kidneys from patients expiring from non-cancer causes, and, likewise, BKV DNA was detected in various tissues by PCR (Chesters et al, 1983;DeMattei et al, 1995).…”

Section: Introductionmentioning

confidence: 96%

Integration of SV40 in human osteosarcoma DNA

1998

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Simian virus 40 (SV40) has been demonstrated in several types of tumors, including osteosarcoma, by polymerase chain reaction (PCR). We detected SV40 sequences by PCR, followed by hybridization, in nine of 35 osteosarcoma tumors and one of 11 osteosarcoma explants. PCR can detect fewer than one virus per cell but gives little detail of the gross structure and abundance of the virus. Analysis by Southern blotting of total DNA from ten osteosarcomas, positive for SV40 by PCR, found viral integration in half of these. Analysis showed integration of one to four copies per cell of rearranged SV40. No SV40 was detectable on blots of the remaining ®ve SV40 + osteosarcomas, perhaps because of the lesser sensitivity of direct hybridization. Inactivation of the p53 and Rb tumor suppressors is a key activity of SV40 T-antigen. Unexpectedly, correlation of these ®ndings with our prior studies indicated that ®ve of ten osteosarcomas positive for SV40 DNA had mutations of p53, and two had deleted Rb. Apparently clonal integration with pre-existing alteration of a tumor suppressor gene, suggests that SV40 may play a role in the ®nal conversion to malignant osteosarcoma.

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“…It persistently infects almost 100% of the human population by early childhood (Gardner, 1973;Padgett, 1981;Do¨rries et al, 1994). It resides in the kidneys in a latent or persistent state, but can be reactivated upon immunosuppression of the host and is associated with hemorrhagic cystitis and polyomavirus nephropathy (Arthur et al, 1986;Hiraoka et al, 1991;Pappo et al, 1996;Randhawa et al, 1999;Li et al, 2002).…”

Section: Introductionmentioning

confidence: 99%