Infection of Chickens Caused by Avian Influenza Virus A/H5N1 Delivered by Aerosol and Other Routes

Сергеев, А. Н.; Demina, O. K.; Pyankov, Oleg V.; Pyankova, Olga G.; Agafonov, A. P.; Kiselev, S. A.; Agranovski, Igor E.; Сергеев, А. А.; Shikov, A. N.; Шишкина, Л. Н.; Safatov, A. S.; Сергеев, А. Н.

doi:10.1111/j.1865-1682.2012.01329.x

Cited by 22 publications

(25 citation statements)

References 26 publications

(50 reference statements)

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“…Furthermore, the frequency of infection and the titers of virus detected were significantly higher in specimens from chickens inoculated with aerosols than with intranasal drops. These findings combined with our previous report (6) are in agreement with those of Sergeev et al (19), who demonstrated that an HP A/Chicken/Kurgan/05/2005 (H5N1) virus administered to chickens in aerosols spread more rapidly along the respiratory tract than the virus inoculated by intranasal drops. Likewise, clinical signs appeared earlier in ferrets infected through inhalation of aerosolized A/Vietnam/1203/2004 (H5N1) virus compared to those infected by intranasal drops (11).…”

Section: Discussionsupporting

confidence: 93%

“…A human clinical study of influenza infection reported that the 50% infectious dose (ID 50 ) by aerosol inhalation was approximately 100-fold less than by inoculation with intranasal drops (24). In accord with that report, studies in chickens have shown that the ID 50 values of both HPAI H5N1 and LPAI H9N2 viruses were substantially lower by aerosol inoculation than by intranasal drops (6,19). Sergeev et al (19) found that aerosolized HPAI H5N1 virus was rapidly spread to various organs via the respiratory infection in chickens.…”

mentioning

confidence: 49%

“…In accord with that report, studies in chickens have shown that the ID 50 values of both HPAI H5N1 and LPAI H9N2 viruses were substantially lower by aerosol inoculation than by intranasal drops (6,19). Sergeev et al (19) found that aerosolized HPAI H5N1 virus was rapidly spread to various organs via the respiratory infection in chickens. To provide comparable information on LPAI virus, we continued to use an H9N2 virus as a model (6).…”

mentioning

confidence: 69%

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Replication of an H9N2 Avian Influenza Virus and Cytokine Gene Expression in Chickens Exposed by Aerosol or Intranasal Routes

Guan

Sharif

2015

Avian Diseases

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This study related the replication of an H9N2 avian influenza virus in chickens to the induction of host acute immune response after aerosol or intranasal inoculation with the virus. On 1, 2, 4, and 7 days postinoculation (dpi), oropharyngeal swabs and tissue specimens of trachea, lungs, spleen, and cecal tonsils were collected for quantification of viral RNA. Expression of cytokine genes in lungs, spleen, and cecal tonsils was quantified by reverse transcriptase-PCR. Virus was detected in all oropharyngeal swabs up to 4 dpi in chickens from both inoculation groups. However, virus was detected more frequently (P<0.05) and in higher titers (1-4 log difference) in specimens of trachea and lungs from the group exposed to aerosols than from the group given intranasal drops. In accord with viral replication findings, expressions of cytokine genes interleukin (IL)-1β (on 2 and 7 dpi), IL-6 (on 2 dpi), and interferon (IFN)-γ (on 2 and 4 dpi) were up-regulated to a significantly higher level (P<0.05) in lung tissue specimens from the group exposed to virus aerosol than from controls that were given saline intranasally. Only IFN-γ on 1 dpi was up-regulated (P<0.05) above that of controls in lung tissue specimens from the group given intranasal drops of virus. In comparison, replication of the virus and induction of IL-1β and IL-6 genes were limited in spleen and cecal tonsil tissue specimens from both groups of chickens inoculated with the virus. These findings indicate that virus administered in aerosols was more efficient than virus administered as intranasal drops, in infecting the lower respiratory tract and in inducing the activity of the cytokine genes. The intense respiratory infection caused by virus aerosols might increase the shedding and transmission of the H9N2 virus in chickens.

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Section: Discussionsupporting

confidence: 93%

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confidence: 49%

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confidence: 69%

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Replication of an H9N2 Avian Influenza Virus and Cytokine Gene Expression in Chickens Exposed by Aerosol or Intranasal Routes

Guan

Sharif

2015

Avian Diseases

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“…The importance of the oro-faecal transmission in spread of AIV is well recognized (Sergeev et al, 2013). However, in the dynamics of HPAI infection, other factors such as the host, virus strain, immunity and environment, which all contribute to the outcomes of H5N1 infections, are not well understood (Spickler et al, 2008;Kwon & Swayne, 2010;Hénaux & Samuel, 2011;Pantin-Jackwood et al, 2013;Wibawa et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the M2e antibody response following highly pathogenic H5N1 avian influenza virus infection and reliability of M2e ELISA for identifying infected among vaccinated chickens

et al. 2015

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A surveillance method able to differentiate between vaccinated and infected poultry is required for those countries that practice vaccination against highly pathogenic avian influenza H5N1. The external domain of the M2 protein (M2e) of influenza virus is a potentially useful differentiating-infected from vaccinated animals (DIVA) antigen but little is known about the M2e antibody response and factors influencing its detection. In this study, the M2e antibody response was characterized in layer birds vaccinated and challenged with an Indonesian H5N1 virus isolate, using a single M2e peptide or four-branched multiple antigenic peptide form of M2e (MAP-M2e) as antigens in two separate ELISAs. Anti-M2e antibodies were absent in chicks with high level of maternal haemagglutination inhibition antibodies and also in all layers vaccinated once, twice or three times with an inactivated commercial H5N1 vaccine. In contrast, anti-M2e antibodies were detected in vaccinated layers challenged with H5N1 virus and their presence was associated with virus isolation and an increase in haemagglutination inhibition titres. The number of birds that developed M2e antibodies, as well as the strength and duration of the M2e antibody response were strongly influenced by the length of the interval between vaccination and challenge. Birds challenged at six weeks after vaccination all developed M2e antibodies by 14 days that lasted until at least 56 days after infection. In birds challenged at two weeks after vaccination, only a proportion of birds developed M2e antibodies by 14 days that lasted only until 28 days post-infection. Both single M2e peptide and MAP-M2e ELISAs had high diagnostic specificity but the diagnostic sensitivity of MAP-M2e ELISA was significantly higher and more effective in detecting M2e antibody in immune and infected birds. The results show that MAP-M2e ELISA would be useful for surveillance in countries using vaccination to control highly pathogenic avian influenza H5N1.

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“…The AAHLnucleoprotein ELISA is a competitive ELISA that had been proved to be sensitive and specific for detection of antibody to the NP of type-A influenza viruses in birds and mammals (Sergeant et al 2009;Sergeev et al 2013) and was used in the surveillance of AI in wild and domesticated birds in Australia (OCVO 2010).…”

Section: Discussionmentioning

confidence: 99%

Circulating H5N1 virus among native chicken living around commercial layer farms

Tarigan¹,

Indriani²,

Ignjatovic³

2015

JITV

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ABSTRAK Tarigan Sejak diterapkannya program vaksinasi kasus penyakit avian influenza (AI) H5N1 pada ayam pembibitan dan ayam ras petelur jarang terdengar. Penelitian in bertujuan menganalisis apakah redanya kasus tersebut berhubungan dengan hilangnya sumber infeksi di sekitar peternakan. Sampel serum dikumpulkan dari 421 ayam buras yang tinggal di sekitar peternakan ayam petelur di Kabupaten Cianjur dan Sukabumi, Jawa Barat pada Maret-April 2014. Antibodi virus AI H5N1 dianalisis dengan uji haemagglutination ihibition (HI), ELISA dan immunoblotting untuk mendeteksi antibodi terhadap haemagglutin virus H5N1, domain eksternal protein M2 (M2E) dan nukleoprotein (NP) virus AI. Sebanyak 8,6% dari ayam buras yang diperiksa seropositif terhadap virus AI berdasarkan satu atau lebih dari uji serologis. Hasil penelitian mengungkapkan bahwa virus H5N1 masih beredar pada ayam buras yang berkeliaran disekitar kandang ayam ras petelur. Sera yang positif dengan uji HI, M2E dan NP ELISA berturut turut 2,4%, 3,3% dan 3,8%. Tidak terlihat kesesuaian antara hasil satu uji dengan uji lainya. Penyebab ketidaksesuaian hasil tersebut diduga karena HI test, MM2e ELISA dan NP ELISA mengukur antibody yang berbeda yang kemunculan dan durasi masing masing antibodi tersebut berbeda. Kenyataan bahwa virus H5N1 masih beredar di sekitar peternakan ayam petelur menunjukkan bahwa ancaman virus AI masih membayangi peternakan ayam komersial dan karena itu vaksinasi dan biosekuriti yang ketat masih dibutuhkan. Soon after the application of vaccination programme against high pathogenic avian influenza H5N1 outbreak of the disease in breeder and commercial layer farms has diminished remarkably in West Java. This study aimed to investigate whether the H5N1 decline is related to the disappearance of source of infection around the farms. Serum samples were collected from 421 native chicken living around commercial layer farms in the Districs of Cianajur and Sukabumi, West Java in March-April 2014. Antibodies to avian influenza virus (AIV) H5N1 were measured using haemaglutination inhibition (HI), ELISAs and immunoblotting that measured presence of antibodies to the haemagglutin of H5N1 strain, as well as the M2e and nucleoprotein (NP) of all avian influenza viruses. Based on the combined results, 8.6% of the native chickens were seropositive to AI virus based on one or more of serological tests. This study provided serological evidence that H5N1 virus was still circulating among native chicken living around commercial layer farms. Many positive sera were however positive for antibodies in one test only: 2.4%, 3.3% and 3.8% by HI test, M2e and NP ELISA, respectively. It could be speculated that the incongruity of the results is due to the fact that HI, MM2e ELISA and NP ELISA all measure different type of antibodies and the duration of these antibodies in serum following infection with H5N1 differ. The fact that H5N1 virus is still circulating around commercial layer farms infers that the commercial farms are still under threat and therefore vaccination ...

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Infection of Chickens Caused by Avian Influenza Virus A/H5N1 Delivered by Aerosol and Other Routes

Cited by 22 publications

References 26 publications

Replication of an H9N2 Avian Influenza Virus and Cytokine Gene Expression in Chickens Exposed by Aerosol or Intranasal Routes

Replication of an H9N2 Avian Influenza Virus and Cytokine Gene Expression in Chickens Exposed by Aerosol or Intranasal Routes

Characterization of the M2e antibody response following highly pathogenic H5N1 avian influenza virus infection and reliability of M2e ELISA for identifying infected among vaccinated chickens

Circulating H5N1 virus among native chicken living around commercial layer farms

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