Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests

Weynants, Vincent; Tibor, Anne; Denoël, Philippe; Saegerman, Claude; Godfroid, Jacques; Thiange, P.; Letesson, Jean‐Jacques

doi:10.1016/0378-1135(95)00153-0

Cited by 78 publications

(54 citation statements)

References 20 publications

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“…It has been known for many years that the OPS from Brucella spp. possesses epitopes, as defined by mAbs, that are not shared with the OPS from Yersinia enterocolitica O:9, which in most other respects has an almost identical structure and is the foremost suspect for the aetiology of most false-positive serological reactions (FPSRs) (48). The main structural features within some of these OPS epitopes have been identified and evaluated (49), although some that have been hypothesised remain elusive (50).…”

Section: Insights Into Anti-brucella Immune Responsesmentioning

confidence: 99%

The quest for a true One Health perspective of brucellosis

Godfroid¹,

DeBolle²,

Roop³

et al. 2014

Rev. Sci. Tech. OIE

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SummaryOne Health is an interdisciplinary collaboration that aims at mitigating risks to human health arising from microorganisms present in non-human animal species, which have the potential to be transmitted and cause disease in humans. Different degrees of scientific collaboration and sectoral integration are needed for different types of zoonotic diseases, depending on the health and associated economic gains that can be expected from a One Health approach. Indeed, mitigating zoonotic risks related to emerging diseases with pandemic potential is different from mitigating risks related to endemic zoonotic diseases like brucellosis. Likewise, management of brucellosis at the wildlife-livestock interface in wildlife conservation areas is in essence different from mitigating transmission of a given Brucella species within its preferential host species, which in turn is different from mitigating the spillover of a given Brucella species to non-preferential host species, humans included. Brucellosis economic models often oversimplify and/or wrongly assess transmission between reservoir hosts and spillover hosts. Moreover, they may not properly value non-market outcomes, such as avoidance of human disease, consumer confidence and conservation biology issues. As a result, uncertainty is such that the economic predictions of these models can be questionable. Therefore, understanding the infection biology of Brucella species is a prerequisite. This paper reviews and highlights important features of the infection biology of Brucella species and the changing epidemiology of brucellosis that need to be integrated into a true One Health perspective of brucellosis.

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Section: Insights Into Anti-brucella Immune Responsesmentioning

confidence: 99%

The quest for a true One Health perspective of brucellosis

Godfroid¹,

DeBolle²,

Roop³

et al. 2014

Rev. Sci. Tech. OIE

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“…However, a natural reservoir of Brucella bacteria in wildlife, e.g., Brucella suis biovar 2 in hares in Denmark, can still pose a threat. Thus, the task of detection and identification remains challenging and requires reliable and sensitive diagnostics tools.The diagnosis of brucellosis is based mainly on serological responses, which can be unspecific owing to cross-reaction or subsensitive reactions in samples from areas with a low or subclinical prevalence of brucellosis (5,7,9,16). Culture-based verification of suspected cases, or pathological findings in clinical cases, can be time-consuming and also can impose a hazard to laboratory personal.…”

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confidence: 99%

Validated 5′ Nuclease PCR Assay for Rapid Identification of the Genus Brucella

et al. 2004

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A real-time, genus-specific 5 nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n ‫؍‬ 23) and did not detect non-Brucella strains (n ‫؍‬ 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.Owing to continuous efforts to control and eradicate brucellosis in domestic animals, the levels of brucellosis have been reduced in many countries (6). However, a natural reservoir of Brucella bacteria in wildlife, e.g., Brucella suis biovar 2 in hares in Denmark, can still pose a threat. Thus, the task of detection and identification remains challenging and requires reliable and sensitive diagnostics tools.The diagnosis of brucellosis is based mainly on serological responses, which can be unspecific owing to cross-reaction or subsensitive reactions in samples from areas with a low or subclinical prevalence of brucellosis (5,7,9,16). Culture-based verification of suspected cases, or pathological findings in clinical cases, can be time-consuming and also can impose a hazard to laboratory personal. Thus, numerous alternative verification methods, based mostly on amplification of universal genes in a conventional PCR, have been reported, although some have produced false-positive results (reviewed in reference 3).The Brucella-specific perosamine synthetase (per) gene is highly conserved and present even in the naturally rough Brucella species B. ovis and B. canis and spontaneously rough strains of B. abortus and B. melitensis (4). The per gene is, with various degrees of similarity, present in a few other bacteria, including Yersinia enterocolitica serotype O:9, Vibrio cholerae O1, Escherichia coli O:157, some serovars of E. hermanni and Stenotrophomonas maltophilia, and Salmonella group N (O:30) (14). To the best of our knowledge, the only real-time PCR work reported is based on hybridization probes used in three different assays for identification of three Brucella species (15). This report describes for the first time the development of a ready-to-go, nonproprietary, open-formula (thus possessing the potential for standardization), 5Ј hydrolysis probe-using real-time PCR assay including an internal amplification control (IAC) for direct verification of suspected Brucella colonies on agar plates.The Brucella-specific primers were designed as previously described (11). The primer combination bruc1-bruc5 was found to be most selective (11). In the present study, three different TaqMan probes (6-carboxyfluorescein [FAM] labeled) were designed and compared in the assay: Bruc1, in close proximity to the 3Ј end of the forward primer; Bruc2, in the middle of the amplified fragment; and Bruc3, within a few nucleotides of the 3Ј end of the reverse primer (Table 1).An artificially created chimerical DNA (12), a second set of primers, and a second TaqMan probe (VIC labeled) (12) were used f...

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“…Phage typing has been a very handy tool for species and biovars characterization alongwith biochemical tests (Godfroid et al, 2002, Singh et al, 2014 There are many brucellosis tests have been published to determine accurate diagnosis of brucellosis. Different serological tests have been developed by keeping various goals in mind but the validation of all these tests is still an issue, the combination of different serological tests with appreciable specificity and sensitivity values can be utilized to know the status of animals (Ariza et al, 1992;Weynants et al, 1996). It is imperative to use both direct and indirect methods for accurate and reliable diagnosis of brucellosis (Carmichael and Greene, 1990;Wanke, 2004).…”

Section: Introductionmentioning

confidence: 99%