Infection of Cats with Molecularly Cloned and Biological Isolates of the Feline Immunodeficiency Virus

Sparger, Ellen E.; Beebe, Amy M.; Dua, Niels; Himathongkam, Sunee; Elder, John H.; Torten, M.; Higgins, Joanne

doi:10.1006/viro.1994.1677

Cited by 45 publications

(58 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notable clinical disease or decreases in CD4: CD8 T-cell ratios were not observed after challenge in either the vaccinated or the unvaccinated control cats (data not shown). The absence of CD4:CD8 T-cell ratio alterations observed in unvaccinated control cats was consistent with previous observations in which CD4 T-cell alterations were associated with later stages of infection with the FIV-PPR biological isolate (35).…”

Section: Resultssupporting

confidence: 92%

Vaccination of Cats with Attenuated Feline Immunodeficiency Virus Proviral DNA Vaccine Expressing Gamma Interferon

Gupta

Leutenegger

Dean

et al. 2007

J Virol

Self Cite

View full text Add to dashboard Cite

Section: Resultssupporting

confidence: 92%

Vaccination of Cats with Attenuated Feline Immunodeficiency Virus Proviral DNA Vaccine Expressing Gamma Interferon

Gupta

Leutenegger

Dean

et al. 2007

J Virol

Self Cite

View full text Add to dashboard Cite

“…All 3 infected, euthanized animals experienced marked declines in CD4 ϩ and CD8 ϩ T cells (Fig. 7), consistent with past observations (11,15,44,57). In the one surviving inoculated cat, Bw, a consistent increase in CD8 ϩ T cells and a relatively stable CD4 ϩ -T-cell number contrasted with its moribund littermates (Fig.…”

Section: Downloaded Fromsupporting

confidence: 90%

“…This initial phase is followed by a protracted asymptomatic period with progressive loss of CD4 ϩ T lymphocytes and a terminal AIDS phase characterized by succumbing to opportunistic infections (31,33,55,56). Considerable variability exists among FIV strains, not only in sequence relatedness but also in pathogenicity in vivo (8,11,15,32,38,44,57).…”

mentioning

confidence: 99%

“…Sodora et al (42) defined the original Petaluma strain (30,46) as a prototype subgroup A and the divergent Japanese TM2 strain (27) as prototype subgroup B. A majority of FIV sequences obtained worldwide were categorized in the A and B subgroups, and these groups vary widely in degrees of pathogenicity in vivo (8,11,15,32,38,44,57). A subtype C was also defined and is the least prevalent FIV subgroup (1,42).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of a Highly Pathogenic Molecular Clone of Feline Immunodeficiency Virus Clade C

Rozieres

Mathiason

Rolston

et al. 2004

J Virol

Self Cite

View full text Add to dashboard Cite

We have derived and characterized a highly pathogenic molecular isolate of feline immunodeficiency virus subtype C (FIV-C) CABCpady00C. Clone FIV-C36 was obtained by lambda cloning from cats that developed severe immunodeficiency disease when infected with CABCpady00C (Abbotsford, British Columbia, Canada). Clone FIV-C36 Env is 96% identical to the noninfectious FIV-C isolate sequence deposited in GenBank (FIV-Cgb; GenBank accession number AF474246) (A. Harmache et al.) but is much more divergent in Env when compared to the subgroup A clones Petaluma (34TF10) and FIV-PPR (76 and 78% divergence, respectively). Clone FIV-C36 was able to infect freshly isolated feline peripheral blood mononuclear cells and primary T-cell lines but failed to productively infect CrFK cells, as is typical of FIV field isolates. Two-week-old specific-pathogen-free cats infected with FIV-C36 tissue culture supernatant became PCR positive and developed severe acute immunodeficiency disease similar to that caused by the uncloned CABCpady00C parent. At 4 to 5 weeks postinfection (PI), 3 of 4 animals developed CD4+-T-cell depletion, fever, weight loss, diarrhea, and opportunistic infections, including ulcerative stomatitis and tonsillitis associated with abundant bacterial growth, pneumonia, and pyelonephritis, requiring euthanasia. Histopathology confirmed severe thymic and systemic lymphoid depletion. Interestingly, the dam also became infected with a high viral load at 5 weeks PI of the kittens and developed a similar disease syndrome, requiring euthanasia at 11 weeks PI of the kittens. This constitutes the first report of a replication-competent, infectious, and pathogenic molecular clone of FIV-C. Clone FIV-C36 will facilitate dissection of the pathogenic determinants of FIV

show abstract

“…In contrast, studies with FIV have documented a significant decline in CD4 count (less than 400\ml) and inversion in CD4\CD8 ration within 4-8 weeks postinoculation, (Bendinelli et al, 1995 ;Dua et al, 1994 ;O'Neil et al, 1995 ;M. H. Myles & E. A. Hoover, unpublished results) though attenuated strains of FIV-Fca may be less capable of altering lymphocyte subset kinetics (Sparger et al, 1994).…”

Section: Flow Cytometrymentioning

confidence: 95%

Infectivity of lion and puma lentiviruses for domestic cats.

VandeWoude

O’Brien²,

Hoover³

1997

Journal of General Virology

View full text Add to dashboard Cite

Infection of Cats with Molecularly Cloned and Biological Isolates of the Feline Immunodeficiency Virus

Cited by 45 publications

References 0 publications

Vaccination of Cats with Attenuated Feline Immunodeficiency Virus Proviral DNA Vaccine Expressing Gamma Interferon

Vaccination of Cats with Attenuated Feline Immunodeficiency Virus Proviral DNA Vaccine Expressing Gamma Interferon

Characterization of a Highly Pathogenic Molecular Clone of Feline Immunodeficiency Virus Clade C

Infectivity of lion and puma lentiviruses for domestic cats.

Contact Info

Product

Resources

About