Infection of a polarized epithelial cell line with wild-type reovirus leads to virus persistence and altered cellular function

Montgomery, Louise B.; Yang, Chiou-Ying; Verdin, Eric; Cahill, Christopher; Maratos–Flier, Eleftheria

doi:10.1099/0022-1317-72-12-2939

Cited by 14 publications

(11 citation statements)

References 45 publications

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“…It is possible that this behaviour reflects cellular alterations and selection of more or less susceptible carrier subpopulations in culture. A mode of changing permissiveness is in line with reports from a reovirus system, where cell modifications were shown to be due to virus persistence (Montgomery et al, 1991). Furthermore, the variability of viral gene expression during persistence can be extended to a related RNA virus group.…”

Section: Persistent Infection With a Variant Of Influenza C/annsupporting

confidence: 84%

Productive and non-productive phases during long-term persistence of influenza C virus

Marschall

Böswald²,

Schuler³

et al. 1993

Journal of General Virology

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Persistent infection with a variant of influenza C/AnnArbor/I/50 virus in MDCK cells has been previously reported. However, the precise molecular mechanism of persistence is still unknown. We show that the release of active progeny virus, as tested for by haemagglutination and acetylesterase profiles, does not take place in freshly seeded MDCK cells. Productive virus replication occurs simultaneously with massive production of structural proteins as shown by immunoprecipitation and immunofluorescence. PCR for the HEF structural proteinencoding segment 4 revealed that positive-sense RNA is present only during virus multiplication whereas negative-sense RNA appears to be constantly detectable, In this study we give initial evidence that influenza C virus can persist in the form of its genomic minus strand RNA, and plus strand transcription, protein synthesis and virus replication remain restricted to productive phases.

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Section: Persistent Infection With a Variant Of Influenza C/annsupporting

confidence: 84%

Productive and non-productive phases during long-term persistence of influenza C virus

Marschall

Böswald²,

Schuler³

et al. 1993

Journal of General Virology

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“…However, detailed data of the kinetics and long-term cultures of (Montgomery et al, 1991), although the cells still exhibited limited cell growth once infected. Furthermore, in our laboratory, MDCK cells were found to be killed upon reovirus infection (Danis and Lemay, 1993;Bisaillon et al, 1999) .…”

Section: Discussionmentioning

confidence: 99%

Transient high level mammalian reovirus replication in a bat epithelial cell line occurs without cytopathic effect

et al. 2013

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Montréal (Québec)H3CSpecific properties of bat cells may thus be in part responsible for the ability of the animals to act as reservoirs for viruses in general and for novel reoviruses in particular. Their peculiar resistance to cell lysis also makes Tb1.Lu cells an attractive model to study the cellular and viral factors that determine the ability of reovirus to replicate and destroy infected cells.2

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“…Assumptions relating to the behaviour of adventitious viruses in MDCK 33016 cell culture were based on data derived from viral growth studies (Section 2.2.1) and supplemented with data extracted from the scientific literature [16][17][18][19][20][21][22][23][24][25]. During generation of the MDCK 33016 viral seed, a minimum of three passages was assumed, each one using a minimum virus dilution of 1:100.…”

Section: Mdck 33016 Cell Culturementioning

confidence: 99%

A quantitative risk assessment of exposure to adventitious agents in a cell culture-derived subunit influenza vaccine

Gregersen

2008

Vaccine

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A risk-assessment model has demonstrated the ability of a new cell culture-based vaccine manufacturing process to reduce the level of any adventitious agent to a million-fold below infectious levels. The cell culture-derived subunit influenza vaccine (OPTAFLU), Novartis Vaccines and Diagnostics) is produced using Madin-Darby canine kidney (MDCK) cells to propagate seasonal viral strains, as an alternative to embryonated chicken-eggs. As only a limited range of mammalian viruses can grow in MDCK cells, similar to embryonated eggs, MDCK cells can act as an effective filter for a wide range of adventitious agents that might be introduced during vaccine production. However, the introduction of an alternative cell substrate (for example, MDCK cells) into a vaccine manufacturing process requires thorough investigations to assess the potential for adventitious agent risk in the final product, in the unlikely event that contamination should occur. The risk assessment takes into account the entire manufacturing process, from initial influenza virus isolation, through to blending of the trivalent subunit vaccine and worst-case residual titres for the final vaccine formulation have been calculated for >20 viruses or virus families. Maximum residual titres for all viruses tested were in the range of 10(-6) to 10(-16) infectious units per vaccine dose. Thus, the new cell culture-based vaccine manufacturing process can reduce any adventitious agent to a level that is unable to cause infection.

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Infection of a polarized epithelial cell line with wild-type reovirus leads to virus persistence and altered cellular function

Cited by 14 publications

References 45 publications

Productive and non-productive phases during long-term persistence of influenza C virus

Productive and non-productive phases during long-term persistence of influenza C virus

Transient high level mammalian reovirus replication in a bat epithelial cell line occurs without cytopathic effect

A quantitative risk assessment of exposure to adventitious agents in a cell culture-derived subunit influenza vaccine

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