Infection and persistence of varicella-zoster virus in lymphoblastoid Raji cell line

Leventon-Kriss, S; Gotlieb-Stematsky, T.; Vonsover, Ami; Smetana, Zehava

doi:10.1007/bf02120813

Cited by 9 publications

(4 citation statements)

References 26 publications

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“…The data presented in this report indicate that VZV can persistently infect the human rhabdomyosarcoma cell line A204. Levonton-Kriss et al (11) previously reported persistent VZV infection in Epstein-Barr virus-transformed Rajii cells; however, to our knowledge VZV-A204 is the first nonlymphoid persistent VZV infection in cell culture. The continued presence of infectious virus and antigen expression in VZV-A204 cells for more than 19 months signifies a persistent infection, although it is possible that latent VZV DNA (full or partial genome) may reside in some of the cells.…”

Section: Discussionmentioning

confidence: 64%

“…Significant numbers of infectious particles cannot be obtained by cell disruption of permissive human diploid embryo fibroblast (HDEF), epithelial, or melanoma cells (7,12). In a recent study, it was reported that VZV can persistently infect Epstein-Barr virus-transformed lymphoblastoid Raji cells (11); however, the interpretation of results with these cells is encumbered by the influence of a resident Epstein-Barr virus genome.…”

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confidence: 99%

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Persistent Varicella-Zoster virus infection in a human rhabdomyosarcoma cell line and recovery of a plaque variant

Iltis¹,

Vette²,

Castellano³

et al. 1982

Infect Immun

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Varicella-zoster virus (VZV) has been found to persistently infect the human rhabdomyosarcoma cell line A204. Infectious center assays and fluorescent antibody staining demonstrated continuous production of infectious VZV and viral antigen. The level of infection determined by fluorescent antibody staining was variable, and usually only a small percentage of the cells were capable of producing plaques in permissive fibroblasts. The extent of infection was similar in cell cultures passaged at split ratios of 1:2 or 1:10 and grown at 33 or 37°C. VZV recovered from A204 cells several months after establishment of the persistent infection had markedly increased syncytia-forming activity as compared with the parental VZV grown in human diploid fibroblast cells and the three monkey kidney-derived cell lines Vero, CV-1, and MA104. The expression of this altered phenotype continued after serial passage of the cell-associated virus in human diploid fibroblast and Vero cells. Consequently, we designated the reisolated VZV as plaque variant A. The buoyant densities of VZV plaque variant A and VZV DNAs in CsCl gradients were indistinguishable.

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Section: Discussionmentioning

confidence: 64%

mentioning

confidence: 99%

Persistent Varicella-Zoster virus infection in a human rhabdomyosarcoma cell line and recovery of a plaque variant

Iltis¹,

Vette²,

Castellano³

et al. 1982

Infect Immun

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“…b The values do not add to 100% because CD4 Ϫ CD8 Ϫ double-negative lymphocytes constitute a small fraction of the cells. relevance to VZV pathogenesis in vivo (6,10,13,16). The capacity to investigate VZV interactions with T lymphocytes in the SCID-hu model will be essential for experiments to identify VZV gene functions that account for this important tropism of the virus.…”

Section: Vol 69 1995mentioning

confidence: 99%

Tropism of varicella-zoster virus for human CD4+ and CD8+ T lymphocytes and epidermal cells in SCID-hu mice

Moffat

Stein

Kaneshima

et al. 1995

J Virol

224

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To investigate the cell tropism and pathogenicity of varicella-zoster virus (VZV) strains, we analyzed VZV replication by using SCID-hu mice that carry human fetal thymus/liver implants under the kidney capsule or as subcutaneous fetal skin implants. MRC-5 cells infected with wild-type VZV or the Oka strain, used in the live attenuated varicella vaccine, were injected into the implants. The implants were surgically removed 2, 7, 14, and 21 days postinfection. The VZV titer from infected thymus/liver implants peaked on day 7 for the wild-type strain and on day 14 for the Oka strain. Histological analysis showed necrotic areas characterized by thymocyte depletion and fibrosis. VZV protein synthesis was detectable by immunohistochemical staining in the necrotic areas and in distant regions that did not show cytopathic changes, and VZV DNA was detected by in situ hybridization in the same distribution. Fluorescence-activated cell sorting analysis of thymocytes harvested at day 7 postinfection showed that VZV proteins were expressed in CD4 ؉ , CD8 ؉ , and CD4 ؉ CD8 ؉ T cells; VZV was cultured from each T-cell subpopulation. The Oka strain had tropism for human cell types similar to that of wild-type VZV. T lymphocytes released infectious VZV, which is a novel and important observation about the replication of this otherwise highly cell associated virus. VZV-infected skin implants exhibited microscopic epidermal lesions that were indistinguishable histologically from the characteristic lesions of varicella. These experiments demonstrate a unique tropism of VZV for human T lymphocytes, explaining its capacity to cause viremia in natural disease, and demonstrate the value of the SCID-hu model for studies of VZV pathogenesis.

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“…In comparison with most cell types, human diploid embryo fibroblasts (HDEF) are highly susceptible to VZV infection and are the cell type most often used in FAMA tests. Levonton-Kriss and co-workers (7) have shown that VZV can infect the continuous lymphoblastoid cell line, Raji. Moreover, they demonstrated VZV antigen in the membranes of infected Raji cells by indirect immunofluorescence.…”

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confidence: 99%

Comparison of the Raji cell line fluorescent antibody to membrane antigen test and the enzyme-linked immunosorbent assay for determination of immunity to varicella-zoster virus

Iltis¹,

Castellano²,

Gerber³

et al. 1982

J Clin Microbiol

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A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and parent contacts in 10 families. Varicella-zoster virus-infected human diploid embryonic fibroblasts and continuous lymphoblastoid cells (Raji cells) were compared as indicator cells in the FAMA test. Equivalent results were

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Infection and persistence of varicella-zoster virus in lymphoblastoid Raji cell line

Cited by 9 publications

References 26 publications

Persistent Varicella-Zoster virus infection in a human rhabdomyosarcoma cell line and recovery of a plaque variant

Persistent Varicella-Zoster virus infection in a human rhabdomyosarcoma cell line and recovery of a plaque variant

Tropism of varicella-zoster virus for human CD4+ and CD8+ T lymphocytes and epidermal cells in SCID-hu mice

Comparison of the Raji cell line fluorescent antibody to membrane antigen test and the enzyme-linked immunosorbent assay for determination of immunity to varicella-zoster virus

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