Infecciones por micobacterias de crecimiento rápido

García-Martos, Pedro; García-Agudo, Lidia

doi:10.1016/j.eimc.2011.09.017

Cited by 28 publications

(18 citation statements)

References 106 publications

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“…One patient was HIV-positive and died as soon as he began treatment for tuberculosis, which indicates the increasing clinical importance of identifying mycobacterial species 43,44 . Several studies have demonstrate the importance of M. fortuitum infections in immunocompromised patients and found that the most prevalent species in these cases include the Mycobacterium avium complex, Mycobacterium kansasii and Mycobacterium fortuitum 10,11,45 . It can therefore be concluded that multiplex PCR has the ability to identify and differentiate species of M. tuberculosis and NTMs and thus can be used as an auxiliary tool in the differential diagnosis of tuberculosis and diseases caused by NTMs.…”

Section: Discussionmentioning

confidence: 99%

“…have been developed to enable rapid and accurate identifi cation of mycobacterial species [10][11][12] . In Brazil, the PRA-hsp65 technique proposed by Telenti et al 13 , and Devallois et al 14 has enabled the identifi cation of several species of NTM, the results of which correlated well with those from biochemical identification [13][14][15][16][17] .…”

Section: Methodsmentioning

confidence: 99%

“…For many years, the identifi cation of mycobacteria was undertaken by gathering phenotypic results and biochemical test results of isolated species in culture medium 9,10 . In the last 20 or 30 years, with the increasing need to identify a greater number of species and also for early diagnosis, new methods…”

Section: Introductionmentioning

confidence: 99%

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Rapid detection and differentiation of mycobacterial species using a multiplex PCR system

Lima

Duarte

Montenegro

et al. 2013

Rev. Soc. Bras. Med. Trop.

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Introduction:The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods: The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential diagnosis of tuberculosis and diseases caused by nontuberculous mycobacteria (NTM) Results: Forty mycobacterial strains isolated from pulmonary and extrapulmonary origin specimens from 37 patients diagnosed with tuberculosis were processed. Using phenotypic and biochemical characteristics of the 40 mycobacteria isolated in LJ medium, 57.5% (n=23) were characterized as the Mycobacterium tuberculosis complex (MTBC) and 20% (n=8) as nontuberculous mycobacteria (NTM), with 22.5% (n=9) of the results being inconclusive. When the results of the phenotypic and biochemical tests in 30 strains of mycobacteria were compared with the results of the multiplex PCR, there was 100% concordance in the identifi cation of the MTBC and NTM species, respectively. A total of 32.5% (n=13) of the samples in multiplex PCR exhibited a molecular pattern consistent with NTM, thus disagreeing with the fi nal diagnosis from the attending physician. Conclusions: Multiplex PCR can be used as a differential method for determining TB infections caused by NTM a valuable tool in reducing the time necessary to make clinical diagnoses and begin treatment. It is also useful for identifying species that were previously not identifi able using conventional biochemical and phenotypic techniques.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Rapid detection and differentiation of mycobacterial species using a multiplex PCR system

Lima

Duarte

Montenegro

et al. 2013

Rev. Soc. Bras. Med. Trop.

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“…Las pruebas bioquímicas respecto a la reducción de nitratos fueron negativas; por el contrario, la producción de arilsulfatasa (3 días) y la hidrólisis de Tween 80 fueron positivas, así como el crecimiento en presencia de NaCl al 5% y en agar MacConkey sin cristal violeta [3][4][5] . La cepa se envió al Centro Nacional de Microbiología-Instituto de Salud Carlos III para su identificación, donde se obtuvo como resultado M. canariasense mediante el análisis de los genes hsp-65 y 16S rRNA 1 .…”

Section: Agradecimientosunclassified

“…La aplicación de técnicas moleculares en la identificación de MCR ha facilitado la caracterización y la diferenciación de las mismas, aumentando el número de especies conocidas, como es el caso de M. canariasense 3,4 . Las MCR presentan una gran variabilidad respecto a su sensibilidad frente a los antimicrobianos, lo que justifica y aconseja la realización de un antimicrobiograma en los aislados de muestras clínicas.…”

Section: Agradecimientosunclassified