Inertial Microfluidic Syringe Cell Concentrator

Xiang, Nan; Shi, Xin; Han, Yu; Shi, Zhi‐Guo; Jiang, Fan; Zhang, Ni

doi:10.1021/acs.analchem.8b02201

Cited by 42 publications

(44 citation statements)

References 49 publications

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“…To better understand the physics of our IM‐centrifuge for cell concentration, we experimentally characterized the particle/cell‐focusing performance in our MFIM chip. The spiral channels in our MFIM chip were designed with simple, two‐loop geometry, and had a low‐aspect‐ratio cross‐section ( AR = channel height ( H )/channel width ( W )) of ∼0.2, according to our previous experiment , which generated a low flow resistance and reduced energy use. The detailed structure parameters of our spiral channels are shown in Supporting Information Table S1.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, our IM‐centrifuge can be employed to concentrate various other bioparticles. The throughput of our IM‐centrifuge is higher than those of other inertial microfluidic concentrators , and is especially suitable for being used under the hand‐powered mode. Further increasing the throughput of our IM‐centrifuge could be realized through multiplexing the cores in the MFIM chip.…”

Section: Resultsmentioning

confidence: 99%

“…Second, nearly all of the current inertial microfluidic concentrators are still fabricated in polydimethylsiloxane (PDMS) using complicated and time‐consuming soft lithography, which prevents the disposable use of inertial microfluidic concentrators in low‐resource settings. To address these issues, we developed in our previous work a single‐core syringe cell concentrator via three‐dimensional (3D) printing . However, the relatively low throughput limited by the single‐core design makes the processing of large‐volume samples difficult, especially for hand‐powered applications (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Low‐cost multi‐core inertial microfluidic centrifuge for high‐throughput cell concentration

Xiang

Shi

et al. 2019

Electrophoresis

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We developed a low-cost multi-core inertial microfluidic centrifuge (IM-centrifuge) to achieve a continuous-flow cell/particle concentration at a throughput of up to 20 mL/min. To lower the cost of our IM-centrifuge, we clamped a disposable multilayer film-based inertial microfluidic (MFIM) chip with two reusable plastic housings. The key MFIM chip was fabricated in low-cost materials by stacking different polymer-film channel layers and double-sided tape. To increase processing throughput, multiplexing spiral inertial microfluidic channels were integrated within an all-in-one MFIM chip, and a novel sample distribution strategy was employed to equally distribute the sample into each channel layer. Then, we characterized the focusing performance in the MFIM chip over a wide flow-rate range. The experimental results showed that our IM-centrifuge was able to focus various-sized particles/cells to achieve volume reduction. The sample distribution strategy also effectively ensured identical focusing and concentration performances in different cores. Finally, our IM-centrifuge was successfully applied to concentrate microalgae cells with irregular shapes and highly polydisperse sizes. Thus, our IM-centrifuge holds the potential to be employed as a low-cost, high-throughput centrifuge for disposable use in low-resource settings.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low‐cost multi‐core inertial microfluidic centrifuge for high‐throughput cell concentration

Xiang

Shi

et al. 2019

Electrophoresis

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“…The use of a large amount of sheath fluid also significantly increases the operating cost. Other approaches mainly rely on the internally or externally imposed forces, such as inertial,[10] viscoelastic [11], electric [12], acoustic [13], and hybrid forces [14,15]. These approaches can accomplish good separation in many circumstances but may have potential drawbacks when the biological particles are handled.…”

Section: Introductionmentioning

confidence: 99%

Separation of micro and sub‐micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis

Xue

Sun

et al. 2020

Electrophoresis

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In the present study, we numerically demonstrate an approach for separation of micro and sub-micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis. The dual streams are constructed by an intermediate sheath flow, after which the negative magnetophoretic force induced by an array of permanent magnets dominates the separation of diamagnetic particles. A simple and efficient numerical model is developed to calculate the motions of particles under the action of magnetic field and flow field. Effects of the average flow velocity, the ratio of sheath fluid flow to sample fluid flow, the number of the magnet pair as well as the position of magnet pair are investigated. The optimal parametric condition for complete separation is obtained through the parametric analysis, and the separation principle is further elucidated by the force analysis. The separation of smaller micro and sub-micro diamagnetic particles is finally demonstrated. This study provides an insight into the negative magnetophoretic phenomenon and guides the fabrication of feasible, low-cost diagnostic devices for sub-micro particle separation. Abbreviations: SE, separation efficiencyColor online: See article online to view Figs. 1-4 in color.

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“…

italicRe = {italicρ}_{normalf} {italicU}_{avg} {italicD}_{normalH} goodbreaknormal/ italicμ

where ρ f is the density of the fluid, D H is the hydraulic diameter of the channel, and µ is the dynamic viscosity of fluid, can reach Re ≈ 400 in comparison with other aforementioned structures, which normally range from 50 to 200 (50 < Re < 200). In the past decade, numerous applications of spiral inertial microfluidics have been shown that can be mainly categorized into separation, e.g., circulating tumor cells (CTCs) enrichment from patient's peripheral blood and concentration or filtration, such as syringe cell concentrator and removal of blood cells for enhancing recovery of viral nucleic acid . Inertial microfluidics throughput is scaled out relatively easily via multiplexing microchannels due to passive focusing of bioparticles, which is dependent only on hydrodynamic forces.…”

Section: Introductionmentioning

confidence: 99%

Scaled‐Up Inertial Microfluidics: Retention System for Microcarrier‐Based Suspension Cultures

Moloudi

Yang

et al. 2019

Biotechnology Journal

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Recently, particle concentration and filtration using inertial microfluidics have drawn attention as an alternative to membrane and centrifugal technologies for industrial applications, where the target particle size varies between 1 µm and 500 µm. Inevitably, the bigger particle size (>50 µm) mandates scaling up the channel crosssection or hydraulic diameter (D H > 0.5 mm). The Dean-coupled inertial focusing dynamics in spiral microchannels is studied broadly; however, the impacts of secondary flow on particle migration in a scaled-up spiral channel is not fully elucidated. The mechanism of particle focusing inside scaled-up rectangular and trapezoidal spiral channels (i.e., 5-10× bigger than conventional microchannels) with an aim to develop a continuous and clog-free microfiltration system for bioprocessing is studied in detail. Herein, a unique focusing based on inflection point without the aid of sheath flow is reported. This new focusing mechanism, observed in the scaled-up channels, out-performs the conventional focusing scenarios in the previously reported trapezoidal and rectangular channels. Finally, as a proof-ofconcept, the utility of this device is showcased for the first time as a retention system for a cell-microcarrier (MC) suspension culture.

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Inertial Microfluidic Syringe Cell Concentrator

Cited by 42 publications

References 49 publications

Low‐cost multi‐core inertial microfluidic centrifuge for high‐throughput cell concentration

Low‐cost multi‐core inertial microfluidic centrifuge for high‐throughput cell concentration

Separation of micro and sub‐micro diamagnetic particles in dual ferrofluid streams based on negative magnetophoresis

Scaled‐Up Inertial Microfluidics: Retention System for Microcarrier‐Based Suspension Cultures

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