Inertial focusing of CTCs in a novel spiral microchannel

Shiriny, Afshin; Bayareh, Morteza

doi:10.1016/j.ces.2020.116102

Cited by 49 publications

(24 citation statements)

References 60 publications

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“…The number of loops in spiral microchannels can be variable, with less pressure drop in the microchannels with fewer loops, but in more loops, higher flow rates can be used for the sample fluid. 50 The first loop is 2 cm in diameter, and the distance between the spirals is 500 mm. In the microchannel manifold, two inlet plugs are provided for the sample fluid and buffer fluid, with a width of 250 mm, which will create an angle of 90°between the two plugs.…”

Section: Detailed Designmentioning

confidence: 99%

Design and experimental investigation of a novel spiral microfluidic chip to separate wide size range of micro-particles aimed at cell separation

Tabatabaei

Targhi

2021

Proc Inst Mech Eng H

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Isolation of microparticles and biological cells on microfluidic chips has received considerable attention due to their applications in numerous areas such as medical and engineering fields. Microparticles separation is of great importance in bioassays due to the need for smaller sample and device size and lower manufacturing costs. In this study, we first explain the concepts of separation and microfluidic science along with their applications in the medical sciences, and then, a conceptual design of a novel inertial microfluidic system is proposed and analyzed. The PDMS spiral microfluidic device was fabricated, and its effects on the separation of particles with sizes similar to biological particles were experimentally analyzed. This separation technique can be used to separate cancer cells from the normal ones in the blood samples. These components required for testing were selected, assembled, and finally, a very affordable microfluidic kit was provided. Different experiments were designed, and the results were analyzed using appropriate software and methods. Separator system tests with polydisperse hollow glass particles (diameter 2–20 µm), and monodisperse Polystyrene particles (diameter 5 & 15 µm), and the results exhibit an acceptable chip performance with 86% of efficiency for both monodisperse particles and polydisperse particles. The microchannel collects particles with an average diameter of 15.8, 9.4, and 5.9 μm at the proposed reservoirs. This chip can be integrated into a more extensive point-of-care diagnostic system to test blood samples.

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Section: Detailed Designmentioning

confidence: 99%

Design and experimental investigation of a novel spiral microfluidic chip to separate wide size range of micro-particles aimed at cell separation

Tabatabaei

Targhi

2021

Proc Inst Mech Eng H

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“…This combination of CTC enumeration and downstream molecular profiling represents a powerful technique for monitoring cancer progression and the personalisation of cancer treatments [ 36 ]. Inertial focusing using microfluidic devices is another label-free alternative that combats these issues via the size- and deformability-based separation of CTCs from whole blood [ 37 ]. Another promising in vivo method for CTC capture, the GILUPI CellCollector ® , facilitates the screening of large volumes of blood using a medical wire coated with antibodies against EpCAM that is placed intravenously to capture CTCs that flow by [ 38 ].…”

Section: Bloodmentioning

confidence: 99%

Clinical Proteomics of Biofluids in Haematological Malignancies

Dunphy

O’Mahoney

Dowling

et al. 2021

IJMS

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Since the emergence of high-throughput proteomic techniques and advances in clinical technologies, there has been a steady rise in the number of cancer-associated diagnostic, prognostic, and predictive biomarkers being identified and translated into clinical use. The characterisation of biofluids has become a core objective for many proteomic researchers in order to detect disease-associated protein biomarkers in a minimally invasive manner. The proteomes of biofluids, including serum, saliva, cerebrospinal fluid, and urine, are highly dynamic with protein abundance fluctuating depending on the physiological and/or pathophysiological context. Improvements in mass-spectrometric technologies have facilitated the in-depth characterisation of biofluid proteomes which are now considered hosts of a wide array of clinically relevant biomarkers. Promising efforts are being made in the field of biomarker diagnostics for haematologic malignancies. Several serum and urine-based biomarkers such as free light chains, β-microglobulin, and lactate dehydrogenase are quantified as part of the clinical assessment of haematological malignancies. However, novel, minimally invasive proteomic markers are required to aid diagnosis and prognosis and to monitor therapeutic response and minimal residual disease. This review focuses on biofluids as a promising source of proteomic biomarkers in haematologic malignancies and a key component of future diagnostic, prognostic, and disease-monitoring applications.

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“…Particular channel geometries create inertial forces that result in a bias towards certain equilibrium positions, a phenomenon which can be used for ordering of cells within a given microfluidic chip. Inertial focusing has been used, for example, in spiral microchannels and serpentine channels [77][78][79][80][81][82]. Inertial effects can also be combined with other hydrodynamic techniques, or flow fractionation techniques as well, such as pinched flow fractionation.…”

Section: Inertial Focusingmentioning

confidence: 99%

Preparation of Tissues and Heterogeneous Cellular Samples for Single-Cell Analysis

Welch¹,

Tripathi²

2021

Sample Preparation Techniques for Chemical Analysis

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While sample preparation techniques for the chemical and biochemical analysis of tissues are fairly well advanced, the preparation of complex, heterogenous samples for single-cell analysis can be difficult and challenging. Nevertheless, there is growing interest in preparing complex cellular samples, particularly tissues, for analysis via single-cell resolution techniques such as single-cell sequencing or flow cytometry. Recent microfluidic tissue dissociation approaches have helped to expedite the preparation of single cells from tissues through the use of optimized, controlled mechanical forces. Cell sorting and selective cellular recovery from heterogenous samples have also gained traction in biosensors, microfluidic systems, and other diagnostic devices. Together, these recent developments in tissue disaggregation and targeted cellular retrieval have contributed to the development of increasingly streamlined sample preparation workflows for single-cell analysis technologies, which minimize equipment requirements, enable lower processing times and costs, and pave the way for high-throughput, automated technologies. In this chapter, we survey recent developments and emerging trends in this field.

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Inertial focusing of CTCs in a novel spiral microchannel

Cited by 49 publications

References 60 publications

Design and experimental investigation of a novel spiral microfluidic chip to separate wide size range of micro-particles aimed at cell separation

Design and experimental investigation of a novel spiral microfluidic chip to separate wide size range of micro-particles aimed at cell separation

Clinical Proteomics of Biofluids in Haematological Malignancies

Preparation of Tissues and Heterogeneous Cellular Samples for Single-Cell Analysis

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