Industrial Waste Utilization for Low-Cost Production of Raw Material Oil Through Microbial Fermentation

Abstract

In view of ever-growing demand of biodiesel, there is an urgent need to look for inexpensive and promising renewable raw material oils for its production. In this context, the aim of this study was to evaluate the potential use of industrial wastes for low-cost production of oils through microbial fermentation. Among the strains tested, Yarrowia lipolytica grew best and produced highest lipid when grown on decanter effluent from palm oil mill. When crude glycerol by-product from a biodiesel plant was added into the effluent as a co-substrate, Y. lipolytica produced a higher biomass of 3.21 g/L and a higher amount of lipid of 2.21 g/L which was 68 % of the dry weight. The scale up and process improvement in a 5-L bioreactor increased the biomass and lipid up to 5.53 and 2.81 g/L, respectively. A semi-continuous mode of operation was an effective mode for biomass enhancement while a fed-batch mode was effective for lipid enhancement. These yeast lipids have potential to be used as biodiesel feedstocks because of their similar fatty acid composition to that of plant oil.

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

“…In another study, crude glycerol with 60% impurity was added to an industrial effluent at a concentration of 4%. Utilization of the feedstock resulted in a lipid titer of 2.21 g/L, which was increased to 2.81 g/L after scale-up [40].…”

“…Utilization of the feedstock resulted in a lipid titer of 2.21 g/L, which was increased to 2.81 g/L after scale-up [40].…”

“…The ratio of C18:1/C18 was about 8, based on the associated peak heights. Similarly, oleic acid and palmitic acid accounted for 44% and 36% of total fatty acids when glycerol was used in the production medium [40]. Papanikolaou et al (2013) reported that oleic acid and palmitic acid constitute 52% and 21% of total fatty acids in the engineered strain devoid of PHD1 gene using glycerol at a concentration of 60 g/L under nitrogen-limiting conditions [37].…”

Lipid production by yeasts growing on biodiesel-derived crude glycerol: strain selection and impact of substrate concentration on the fermentation efficiency

“…; Duarte et al . ,b; Kitcha and Cheirslip ; Louhasakul and Cheirsilp ; Wensel et al . ), or as a substrate for the production of metabolic compounds (such as citric acid, acetic acid, polyols, etc.)…”

The Ways of Increasing Bioconversion of Crude Glycerol in Biosurfactants of Rhodococcus erythropolis ІМВ Аc-5017, Acinetobacter calcoaceticus ІМВ В-7241 and Nocardia vaccinii IМВ В-7405

“…В то же время, учитывая объемы производства биодизеля в мире -более 11 млн т в 2008 году с ежегодным последующим увеличением на 8−10 % [15], а также количество обра-зующегося в качестве отходов технического глицерина -10 % от получаемого биодизеля [13], становится понятно, что для эффективного использования такого отхода в качестве субстрата в биотехнологических процессах его содержание в среде культивирования продуцентов прак-тически ценных микробных метаболитов должно быть как можно выше.…”