Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S

Liu, Yuhua; Hu, Xiaoke; Liu, Hui

doi:10.1016/j.ibiod.2015.11.007

Cited by 12 publications

(4 citation statements)

References 31 publications

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“…HC8-3S (GenBank accession number MN216306), which were both isolated from the crude oil-contaminated marine sediments of Bohai Bay, China. Detailed information about these two strains in crude oil utilization could also be found in our previous study (Lin et al, 2014;Liu et al, 2016;Chen et al, 2017).…”

Section: Bacteria Crude Oil Media and Chemicalsmentioning

confidence: 91%

Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers

Chen

Kong

et al. 2020

International Biodeterioration & Biodegradation

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Bioremediation is an attractive strategy of utilizing bacteria to remove crude oil contaminants. In this study, two salt-tolerant crude oil-degrading and biosurfactant-producing bacteria, Dietzia sp. CN-3 and Acinetobacter sp. HC8-3S, were functionally combined to construct a bacterial consortium. The consortium achieved 95.8% degradation efficiency of crude oil in 10 days and various n-alkanes, cycloalkanes, branched alkanes and aromatic hydrocarbons were all depleted more effectively than single strains. Functional optimization of the consortium degraded crude oil efficiently in a wide range of pH (4-10) and salinity (0-120 g L − 1). Furthermore, two alkane hydroxylase genes, alkB in CN-3 and alkM in HC8-3S, were cloned and their expression were examined by real-time quantitative polymerase chain reaction, indicating that alkB was more prominent in long-chain alkanes (C 20 , C 24 and C 26) utilization and alkM played crucial roles in medium-and long-chain alkanes (C 14 , C 16 , C 20 , C 24 and C 26) degradation. In soil microcosms artificially contaminated with crude oil and bioaugmented with the consortium, 58.3% of total petroleum hydrocarbons were depleted after 60 days and the degradation rate (485.8 mg kg − 1 d − 1) was higher than those reported in previous studies. Consequently, the consortium is a promising candidate in crude oil bioremediation.

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Section: Bacteria Crude Oil Media and Chemicalsmentioning

confidence: 91%

Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers

Chen

Kong

et al. 2020

International Biodeterioration & Biodegradation

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“…Acinetobacter sp. strains are most often found in contaminated habitats [3] and act as the best degraders for the bioremediation of soil polluted by crude oil [14]. The marine crude oil-degrading bacterium Acinetobacter sp.…”

Section: Introductionmentioning

confidence: 99%

“…The marine crude oil-degrading bacterium Acinetobacter sp. strains have been cultured on an industrial scale [14]. Study on the multiple catabolic capacity of 31 isolates belonging to the genus Acinetobacter has shown multiple degradation of hydrocarbons, dibenzothiophene, fluorene, dibenzofuran, benzyl sulfide, and sodium benzoate [15].…”

Section: Introductionmentioning

confidence: 99%

Crude Oil Degrading Fingerprint and the Overexpression of Oxidase and Invasive Genes for n-hexadecane and Crude Oil Degradation in the Acinetobacter pittii H9-3 Strain

Wang

Liu

2019

IJERPH

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A crude oil-degrading bacterium named strain H9-3 was isolated from crude oil contaminated soil in the Northeastern area of China. Based on its morphological characteristics and 16S rDNA sequence analysis, strain H9-3 is affiliated to Acinetobacter pittii in the group of Gammaproteobacteria. The strain was efficient in removing 36.8% of the initial 10 g·L−1 of crude oil within 21 days. GC-MS was performed and a preference was shown for n-C10, n-C11, i-C14, i-C17, i-C34, n-C12, n-C13, n-C14, n-C27, n-C32 and i-C13, over n-C16, n-C18–C22, n-C24–n-C31, and n-C36. This can be regarded as the specific fingerprint for crude oil degradation by strain H9-3 of Acinetobacter pittii. In addition to crude oil, it was shown that soybean oil and phenols can be utilized as carbon sources by strain H9-3. It was also shown that aniline and α-naphthol cannot be utilized for growth, but they can be tolerated by strain H9-3. Methylbenzene was neither utilized nor tolerated by strain H9-3. Although n-hexadecane was not preferentially consumed by strain H9-3, during culture with crude oil, it could be utilized for growth when it is the sole carbon source. The degradation of some branched alkanes (i-C14, i-C17 and i-C34) and the preferential degradation of crude oil over phenols could be used as a reference for distinguishing A. pittii from A. calcoaceticus. The difference in gene expression was very significant and was induced by diverse carbon sources, as shown in the qRT-PCR results. The oxidation and adhesion events occurred at high frequency during alkane degration by Acinetobacter pittii strain H9-3 cells.

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“…It was reported that 1:4 diluted seawater could increase 10% biomass of Bacillus thuringiensis in 72 hour at 30°C (Ghribi et al, 2007). In addition, crude oil-degrading bacteria isolated from sediment could grow in variety concentration of salt from 10 to 30 g/L (Liu et al, 2016). This bacterial strain entered to stationary phase after 9 hours in 15% SB medium with diluted and non-diluted seawater.…”

Section: Effect Of Seawater Concentration and Sugarcane Molasses Conc...mentioning

confidence: 94%

DEVELOPMENT OF LIQUID BACTERIAL FORMULATION OF Exiguobacterium sp. AO-11 FOR BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER

Boubpha

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Bioremediation is an effective, low cost and environmental friendly technique to clean up crude oil-contaminated environment. This study aimed to develop the ready to use liquid bacterial formulation of petroleum hydrocarbon-degrading Exiguobacterium sp. AO-11 which was isolated from marine sediment, for bioremediation of crude oil contaminated-seawater. Strain AO-11 was evaluated for the degradation of crude oil in various environmental conditions. The results revealed that this strain could degrade crude oil in various environmental conditions including pH 6 to 9, salinity 8 to 45.4 ppt, temperature 25 to 37oC and crude oil concentration 0.25 to 1.5% (v/v). In order to reduce the cost of bacterial formulation, agro-industrial byproducts, soybean oil mill dry sludge 15% (w/v) (SB) in 1:4 diluted seawater based medium was used for bacterial cultivation and it could enhance bacterial cell growth from 6.07±0.06 Log CFU/ml up to 8.6±0.02 Log CFU/ml in 9 hours. For preparation of liquid formulation, phosphate buffer was selected as a suitable suspension solution which could preserve AO-11 survival at 86.7±1.4% in 30 days and maintain crude oil degradation in liquid cultivation at more than 80%. Protective agent (PEG 1%) was selected for prolonged storage and it could preserve AO-11 up to 72.4±0.2% and 58.7±0.7% at 4ºC and 30ºC in 60 days, respectively. The preliminary estimation of the costs of AO-11 liquid formulation was at 68 baht/L. Moreover, 30-day stored AO-11 formulation could degrade 0.5% (v/v) crude oil in seawater up to 61±5.2%. This study revealed the potential of using agro-industrial waste based cultivation medium for bacterial cell production. Furthermore, liquid bacterial formulation was developed and it has potential to be used for bioremediation of crude oil-contaminated seawater.

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Industrial-scale culturing of the crude oil-degrading marine Acinetobacter sp. strain HC8-3S

Cited by 12 publications

References 31 publications

Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers

Enhanced biodegradation of crude oil by constructed bacterial consortium comprising salt-tolerant petroleum degraders and biosurfactant producers

Crude Oil Degrading Fingerprint and the Overexpression of Oxidase and Invasive Genes for n-hexadecane and Crude Oil Degradation in the Acinetobacter pittii H9-3 Strain

DEVELOPMENT OF LIQUID BACTERIAL FORMULATION OF Exiguobacterium sp. AO-11 FOR BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER

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