Industrial Enzymes: Enzymes in Nonfood Applications

Antrim, Richard L.; Büchert, Johanna; Burrows, Heidi; Herbots, Ivan; Kottwitz, B.; Lenting, H.B.M.; Niku‐Paavola, Marja‐Leena; Reilly, Peter J.; Suurnäkki, Anna; Viikari, Liisa

doi:10.1002/3527602135.ch5b

Cited by 8 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lipases improve the washing capacity of the proteasecontaining detergents and improve the removal of fatty food stains and sebum from fabrics, which are difficult to remove under normal washing conditions [3]. Another important factor is that using hydrolytic enzymes (lipases, proteases and amylases) in detergent formulations allows laundering at lower temperatures, and thus reduces energy expenditure [4].…”

Section: Introductionmentioning

confidence: 99%

Effect of Surfactants and Polyethylene Glycol on the Activity and Stability of a Lipase from Oilseeds of Pachira aquatica

Polizelli

Tiera

Bonilla-Rodriguez

2008

J Americ Oil Chem Soc

View full text Add to dashboard Cite

Lipases from oilseeds have a great potential for commercial exploration as industrial enzymes. Lipases are used mixed with surfactants in cleaning and other formulated products, and accordingly, both components must be compatible with each other. This work presents the results of the effects of anionic, cationic and nonionic surfactants, polyethylene glycol and urea on the activity and stability of a lipase extracted of oilseeds from Pachira aquatica. The enzyme was purified and the spectrophotometric assays were done using p-nitrophenyl acetate (p-NPA) as substrate pH 7.5 and 25°C. The activity was significantly enhanced by the cationic surfactant CTAB. Bile salts increased the lipase activity in the tested concentration range, whereas anionic and nonionic surfactants showed an inhibitory effect. Aqueous solutions of PEG activated the lipase and maximum activation (161%) occurred in PEG 12,000. This effect on lipase that can be due to exposition of some hydrophobic residues located in the vicinity of the active site or aggregation.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Surfactants and Polyethylene Glycol on the Activity and Stability of a Lipase from Oilseeds of Pachira aquatica

Polizelli

Tiera

Bonilla-Rodriguez

2008

J Americ Oil Chem Soc

View full text Add to dashboard Cite

show abstract

“…Each blood sample was divided into seven parts, one mL each spotted on seven pieces of autoclaved white cotton fabric (3 Â 3 cm) (13). The cotton fabric was previously tested for blood DNA without the addition of samples as negative control.…”

Section: Blood and Semen Sample Processingmentioning

confidence: 99%

Thermal Effects on DNA Degradation in Blood and Seminal Stains: Forensic View

Hady¹,

Thabet²,

Ebrahem³

et al. 2021

Academic Forensic Pathology

View full text Add to dashboard Cite

Forensic investigations using DNA analysis have been grown rapidly. Samples retrieved from crime scene may be exposed to different conditions before proceeding. This study aimed to evaluate the effect of different grades of temperature and burn on DNA extraction and typing. Methods: Seven mL of blood and four mL of semen were collected from each volunteer. Effects of temperature grades (100 °C, 50 °C, 37 °C, 4 °C, −20 °C, and burn) on blood and seminal stain were tested. Results: Bloodstains exposed to temperature grades 100 °C, 50 °C, 37 °C, 4 °C, and −20 °C can be identified using preliminary test while burnt blood stain cannot. Seminal stains exposed to temperature grades 37 °C, 4 °C, and −20 °C can be identified by Florence test while those exposed to 100 °C, 50 °C, and burn cannot. Blood and seminal stains exposed to temperature grades 100 °C, 50 °C, and burn show marked reduction in DNA concentration while maximum DNA conc could be recovered from stains exposed to temperature grade temperature. Both blood and seminal DNA was affected only in case of burn without significant difference between THO1 and Amelogenin primers. Conclusion: High environmental temperature affect the quantity of extracted DNA from different stains but less effect on the quality of extracted DNA. Burn affects both preliminary test, DNA quantity, and quality in stains.

show abstract

“…Each blood sample was divided into four parts, one ml each, spotted on four pieces of autoclaved white cotton fabric of surface area 3×3 cm (Antrim et al, 2004). The cotton fabric was tested as a negative control for blood DNA without the addition of blood samples.…”

Section: B-blood and Semen Sample Processing;mentioning

confidence: 99%

Role of aging in identification of DNA extracted from blood and seminal stains

Abdel-Hady

Thabet

Yassa

et al. 2019

Zagazig Journal of Forensic Medicine

View full text Add to dashboard Cite

DNA investigation plays a vital role than ever before in criminal cases as samples retrieved from crime scene may be exposed to different conditions before proceeding that can lead to degradation of DNA.One of these conditions is the aging effect. The aim of this study was to evaluate the effect of aging process on DNA extraction and typing from blood and seminal stains.The present study was done on twenty male volunteers after written consent; four ml of blood and two ml of semen were collected from the same volunteer and spotted on pieces of cotton fabric. The effect of aging (one, three and six months) extraction of DNA from blood and seminal stains were tested. DNA concentration was measured by spectrophotometer and analyzed by using gel electrophoresis. It was shown that blood and seminal stains left at room temperature for one, three and six months can be identified using presumptive tests. DNA concentration was significantly reduced in blood and seminal stains left for one, three and six months before processing. Quality of DNA was not affected by storage length and DNA fragments could be identified except for bloodstains left for six months which cannot detected by Amelogenin primer. In conclusion; DNA can be extracted from blood and seminal stains left for one, three and six months in room temperature. It is recommended to expose the samples to longer periods more than 6 months at room temperature. Different primers can be used for further research. Other types of samples can be used in further studies rather than blood and semen as saliva and teeth. Key word: blood-semen-degradation-DNA-Amelogeninprimers. I

show abstract

Industrial Enzymes: Enzymes in Nonfood Applications

Cited by 8 publications

References 0 publications

Effect of Surfactants and Polyethylene Glycol on the Activity and Stability of a Lipase from Oilseeds of Pachira aquatica

Effect of Surfactants and Polyethylene Glycol on the Activity and Stability of a Lipase from Oilseeds of Pachira aquatica

Thermal Effects on DNA Degradation in Blood and Seminal Stains: Forensic View

Role of aging in identification of DNA extracted from blood and seminal stains

Contact Info

Product

Resources

About