Industrial Applications of Endoglucanase Obtained from Novel and Native Trichoderma atroviride

Abstract

An endo-β-1,4-glucanase (EG), produced under submerged fermentation by local isolate Trichoderma atroviride, was purified using ammonium sulfate precipitation, column and ion exchange chromatography with 55.16 fold and a specific activity of 30.9 EU mg -1 . The studies of PAGE, SDS-PAGE and zymogram test have been carried out. The EG had optimum activity at pH 5.0 and 50 °C respectively. Using CMC as substrate, the enzyme showed maximum activity (V max ) of 6.7 (µmol glucose min . While the EG activity was activated by NaCl, inhibited by MgCl 2 and MgSO 4 . Otherwise, Tween 80, Triton X-100 and the total saponins known as biosurfactants enhanced the EG activity. The obtained EG had low K m and high thermal stability. Additionally, usability of the EG in biotechnological application was investigated. The results showed that the EG had potentially sufficient effects on denim fabric and pretreated lignocellulosic wastes.

Gamma Rays Mediated Mutagenesis of Super Koji (Aspergillus oryzae) to enhance the Thermostability and Production of CMCases: Process Scale-up at Pilot Level

“…Submerged fermentation is the widely used method for commercial production of cellulases due to the easy recovery of extracellular enzymes, although submerged fermentation can result in reduction of enzyme activity and dilution of enzymes [53]. In the current study the A.oryzae mutant 60(5) was grown in 10 L fermenters under submerged conditions and maximum enzyme units obtained were 913 U dl -1 by using Raw Maize Starch as carbon source (2% w/v).…”

Purification, characterization and thermodynamic analysis of cellulases produced from Thermomyces dupontii and its industrial applications

“…Enzyme activity was ceased by keeping the samples at 80 °C for about 10 min. The samples were then carefully washed with tap water and left at room temperature for drying ( Sahin et al, 2016 ).…”