Induktion von Knochengewebe auf unterschiedlichen Matrizes: Eine In-vitro- und In-vivo-Pilotstudie in der SCID Maus

Kasten, Philip; Luginbühl, Reto; Vogel, J.; Niemeyer, P; Weiß, Stefan; Griensven, Martijn van; Krettek, Christian; Bohner, Marc; Bösch, U.; Tonak, Marcus

doi:10.1055/s-2004-820342

Cited by 12 publications

(5 citation statements)

References 8 publications

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“…Very little data has been published to date on the use of this cement in the form of granules for critical-size defects [19,20,29]. According to Bohner and Baumgart, the in vivo resorption rate of porous granules can be predicted to be much faster than in dense blocks or prismatic objects made out of the same material [30].…”

Section: Discussionmentioning

confidence: 99%

“…There have, however, so far only been a limited number of clinical and experimental studies conducted to test the feasibility of these CaP cement-granules, which are moreover difficult to compare with our animal model. In vitro cell culture tests [34-36] and only few in vivo implantations have been performed [19,20]. However, there is presently no in vivo study clearly demonstrating differences in performance between CaP cement-granules and sintered ceramic granules [14].…”

Section: Discussionmentioning

confidence: 99%

“…Now using appropriate techniques granules can be made from CaP cements with very high surface areas that might be potent drug carriers or biologically very reactive [14-16]. In spite of the many positive features of CaP cements, there have only been a few in vivo studies in which these have been used in the form of granules to date [12,14,16,19,20]. …”

Section: Introductionmentioning

confidence: 99%

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The progress of early phase bone healing using porous granules produced from calcium phosphate cement

et al. 2010

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Objective Bone grafting is a vital component in many surgical procedures to facilitate the repair of bone defects or fusions. Autologous bone has been the gold standard to date in spite of associated donor-site morbidity and the limited amount of available donor bone. The aim of this study was to investigate the progress of bone regeneration and material degradation of calcium phosphate granules (CPG) produced from a calcium phosphate self-setting cement powder compared to the use of autologous bone grafting in the treatment of "critical size defects" on load-bearing long bones of minipigs. Methods A critical size defect in the tibial metaphysis of 16 mini-pigs was filled either with autologous cancellous graft or with micro- and macroporous carbonated, apatic calcium phosphate granules (CPG) produced from a calcium phosphate self-setting cement powder. After 6 weeks, the specimens were assessed by X-ray and histological evaluation. The amount of new bone formation was analysed histomorphometrically. Results The semi-quantitative analysis of the radiological results showed a complete osseous bridging of the defect in three cases for the autograft group. In the same group five animals showed a beginning, but still incomplete bridging of the defect, whereas in the CPG group just two animals developed this. All other animals of the CPG group showed only a still discontinuous new bone formation. Altogether, radiologically a better osseous bridging was observed in the autograft group compared to the CPG group. Histomorphometrical analysis after six weeks of healing revealed that the area of new bone was significantly greater in the autograft group concerning the central area of the defect zone (p < 0.001) as well as the cortical defect zone (p < 0.002). All defects showed new bone formation, but only in the autograft group defects regenerated entirely Conclusions Within the limits of the present study it could be demonstrated that autologous cancellous grafts lead to a significantly better bone regeneration compared to the application of calcium phosphate granules (CPG) produced from a calcium phosphate self-setting cement powder after 6 weeks. In the early phase of bone-healing, the sole application of CPG appears to be inferior to the autologous cancellous grafts in an in vivo critical size defect on load-bearing long bones of mini-pigs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The progress of early phase bone healing using porous granules produced from calcium phosphate cement

et al. 2010

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“…As shown in Fig. 47 On the other hand, it has been demonstrated that a highly interconnected pore network with high porosity could benefit cell growth, transport of nutrients and metabolic waste, the deposit of cellular matrix and the in growth of newly formed tissues. This result indicated that 20% DBM/SF scaffolds had the most prominent effect on cell attachment and growth.…”

Section: Discussionmentioning

confidence: 99%

Delivery of demineralized bone matrix powder using a salt-leached silk fibroin carrier for bone regeneration

et al. 2015

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Demineralized bone matrix (DBM) has been widely used for bone regeneration due to its osteoinductivity and osteoconductivity. However, the use of DBM powder is limited due to the difficulties in handling, the tendency to migrate from graft sites and the lack of stability after surgery. In this study, a mechanically stable, salt-leached porous silk fibroin carrier was used to improve the handling properties of DBM powder and to support the attachment, proliferation and osteogenic differentiation of rat bone marrow derived mesenchymal stem cells (rBMSCs). The DBM-silk fibroin (DBM/SF) scaffolds were fabricated with different contents of DBM powder (0%, 10%, 20%, 40% and 80% DBM/SF scaffolds). It was found that the DBM/SF scaffolds could form a stable composite preventing the migration of DBM powder. Moreover, the microarchitecture and mechanical properties of the scaffolds were influenced by the DBM powder. rBMSCs were seeded on the DBM/SF scaffolds and cultured for 14 days. Cell proliferation assays and cell morphology observations indicated that 20% DBM/SF scaffolds exhibited good cell attachment and proliferation. In addition, compared with the other groups, the cellular function was more actively exhibited on 20% DBM/SF scaffolds, as evident by the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis for osteoblast-related gene markers (e.g. COL1A1, ALP and cbfa1), the immunocytochemical evaluations of osteoblast-related extracellular matrix components (e.g. COL1A1, OCN and ONN) and the ALP activities. All the data suggested that DBM powder could be delivered using a silk fibroin carrier with improved handling characteristics and that 20% DBM/SF scaffolds had great potential as osteogenesis promoting scaffolds for successful applications in bone regeneration. † Electronic supplementary information (ESI) available: Immunocytochemistry staining video (10Â objective) of rBMSCs cultured on 20% DBM/SF scaffolds for 7 (Video S1) and 14 days (Video S2). The confocal images were captured from the surface to a depth of 165 mm with increments of 3 mm. The videos were created by the 3D projections module of LAS AF to observe the 3D morphologies of the cellscaffold constructs. See

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“…Alkaline phosphatase (ALP) staining was performed as described in the manufacturer's instructions (Rainbow, Shanghai, China). Meanwhile, ALP activity was determined at 405 nm using p-nitrophenylphosphate (pNPP) (Sigma-Aldrich) as the substrate, 19 while the total protein content was determined by the protein assay kit according to the protocol provided. 20 The ALP activity OD values were nally normalized to the total protein content OD values correspondingly.…”

Section: Osteogenic Differentiation Of Hbmscsmentioning

confidence: 99%

Hydrothermal fabrication of magnetic mesoporous carbonated hydroxyapatite microspheres: biocompatibility, osteoinductivity, drug delivery property and bactericidal property

et al. 2014

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Induktion von Knochengewebe auf unterschiedlichen Matrizes: Eine In-vitro- und In-vivo-Pilotstudie in der SCID Maus

Abstract: All three matrices promoted BMSC proliferation and differentiation to osteogenic cells in vitro. Human BMSC on CDHA showed signs of osteogenic differentiation after subcutaneous implantation into SCID mice.

Cited by 12 publications

References 8 publications

The progress of early phase bone healing using porous granules produced from calcium phosphate cement

The progress of early phase bone healing using porous granules produced from calcium phosphate cement

Delivery of demineralized bone matrix powder using a salt-leached silk fibroin carrier for bone regeneration

Hydrothermal fabrication of magnetic mesoporous carbonated hydroxyapatite microspheres: biocompatibility, osteoinductivity, drug delivery property and bactericidal property

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