Induksi Tunas dan Perakaran Bambu Kuning Bambusa vulgaris secara in vitro

Astuti, Puji

doi:10.24252/bio.v2i2.476

Cited by 2 publications

(1 citation statement)

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“…In this research, combination of 10 mg/l IBA + 5 mg/l NAA effectively induced roots formation. IBA 10 mg/l has been used also in rooting medium of Garcinia mangostana L. (Joni et al, 2015), mangosteen (Roostika et al, 2008), Bambusa vulgaris (Astuti, 2014) and Bambusa tulda (Sharma & Sarma, 2013), while the application of 5mg/l NAA concentration has been used in Bambusa tulda (Sharma & Sarma, 2013). Auxin affects the plant tissue by two ways: 1) Induction of the secretion of H + ion from the cell to the cell wall, make the cell wall being acid.…”

Section: Root Inductionmentioning

confidence: 99%

In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood

2018

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A diversion of raw material from wood to bamboo is necessary. In vitro culture of bamboo can be used to provide a high number of seedling. The aim of this study was to increase the multiplication of a high quality Bambusa balcooa as a wood alternative material. Part of plants used was the sterile axillary shoot. The explants were planted on MS0 medium for 2 weeks and later on multiplication medium MS+0.3 mg/l BAP + 0.3mg/l TDZ. The shoots obtained were fragmented into clusters (3-5 shoots) used for the next multiplication stage using five different medium formulas: (1) MS0; MS containing: (2) 0.1 mg/l BAP, (3) 0.3 mg/l BAP, (4) 0.1 mg/l BAP + 0.1 mg/l TDZ and (5) 0.3 mg/l BAP + 0.1 mg/l TDZ. The results showed that MS medium containing 0.1 mg/l BAP + 0.1mg/l TDZ was the best medium for B. balcooa propagation. The shoots produced from aforementioned medium had a better quality compared to the other medium. Forty days after planting, the average number of shoots in this medium was 14.25. MS medium containing 0.3 mg/l BAP + 0.1 mg/l TDZ produced the highest number of shoot but in lower quality. Rooting medium containing 10 mg/l IBA + 5 mg/l NAA produced 9-16 root in 8 weeks. Vermicompost was more prevalent for the acclimatization of B. balcooa compared to compost. The use of B.balcooa resulted in in vitro propagation as a substitute alternative for wood is expected to save the environment from illegal logging.

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Section: Root Inductionmentioning

confidence: 99%

In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood

2018

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Propagation of high-density carbon bamboo to support smart ecotourism at the Lake Toba, North Sumatera

Ermawar

Hartati

Harmoko

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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Appropriate propagation technology to promote bamboo sustainability is important in supporting conservation and industrial needs. As part of smart ecotourism package based on the integrated charcoal and bamboo tourism at Lake Toba, this research aimed to develop appropriate propagation for the selected bamboo species. Various bamboo species were collected and propagated by both ex vitro and in vitro propagation. Survival rate of the selected bamboo shoots after 3 months in the ex vitro planting medium was in the range of 25 to 50% of 10 – 25 planted shoots, from the highest to the lowest rate were Bambusa sp., Dendrocalamus asper, Oxythenantera abyssinica, and Balcoa 36. Meanwhile, the efficiency of the shoot formation in the in vitro medium was in the range of 7 to 13%, from the highest to the lowest percentage were D. asper, Gigantochloa cf robusta, O. abyssinica and Bambusa sp. Various types of explant (leaves disc, shoots tips, and nodal segment) from G. robusta and O. abyssinica were induced in six different combinations and concentrations of induction callus medium. Explant from nodal segment of G. robusta and O. abyssinica showed a positive response, i.e., 6% and 20% consecutively, in the Murashige & Skoog (MS) medium containing 4 mg/L of 2,4-D and 0.5 mg/L of IBA. The nodal segment explant of G. robusta was also able to form callus (13%) on the MS medium containing 6 mg/L of kinetin. A similar efficiency percentage was also obtained from O. abyssinica nodal segment explant on the MS medium containing 3 mg/L of 2,4-D and 2 mg/L of kinetin. This research opens a possibility of cultivating bamboo by in vitro propagation and generating new variety of bamboo with desirable characteristics.

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Induksi Tunas dan Perakaran Bambu Kuning Bambusa vulgaris secara in vitro

Cited by 2 publications

References 0 publications

In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood

In Vitro Propagation of Bambusa balcooa as Alternative Material of Wood

Propagation of high-density carbon bamboo to support smart ecotourism at the Lake Toba, North Sumatera

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