INDUKSI KALUS TANAMAN KRISAN (Chrysanthemum morifolium) DENGAN PEMBERIAN BENZIL AMINO PURIN (BAP) DAN DICHLOROFENOKSI ACETIL ACID (2,4 D)

Hariyati, Maspan; Bachtiar, I; Sedijani, Prapti

doi:10.29303/jppipa.v2i1.37

Cited by 7 publications

(8 citation statements)

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“…This tissue develops into small spheres that are increasingly clear and cloudy white or yellowish white to form callus aggregates. This condition is in accordance with the statement [12] that callus growth begins with swelling of the explants then on the slices a clear or yellowish white callus will appear and at last the whole surface of the explant will be covered by callus The average callus formation time was 14 DAI. The increase of 0.4 mg. l -1 TDZ in jatropha plant explants was able to produce a shorter callus formation time.…”

Section: Percentage Of Callus Induction and Callus Appearancesupporting

confidence: 88%

Indirect organogenesis of Aceh patchouli leaf explants (Pogostemon cablin Benth) by in vitro

Puspita

Efendi

Zakaria

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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The Aceh patchouli plant (Pogostemon cablin. Benth) is a plantation plant that produces essential oils with great potential, known as patchouli oil. The use of in vitro culture techniques on patchouli plants needs to be developed to meet the needs of patchouli seeds. This study aimed to analyze the effect of plant growth regulators (PGRs) cytokinins 6-Benzyl Amino Purine (BAP) and Thidiazuron (TDZ) and auxin 1-Naphthaleneacetic Acid (NAA) on the organogenesis ability of Aceh patchouli leaf explants var. Lhokseumawe. The treatments analyzed were P1 = MS; P2 = BAP 0.75 mg.l−1, P3 = BAP 1.0 mg.l−1, P4 = TDZ 0.75 mg.l−1, P5 = TDZ 1.0 mg.l−1, P6 = NAA 0.5 mg.l−1, P7 = NAA 1.0 mg.l−1, P8 = BAP 0.75 mg.l−1 + NAA 0.5 mg.l−1, P9 = BAP 0.75 mg.l−1 + NAA 1.0 mg.l−1, P10 = BAP 1.0 mg.l−1 + NAA 0.5 mg.l−1, P11 = BAP 1.0 mg.l−1 + NAA 1.0 mg.l−1, P12 = TDZ 0.75 mg.l−1 + NAA 0.5 mg.l−1, P13 = TDZ 0.75 mg.l−1 + NAA 1.0 mg. l−1, P14 = TDZ 1.0 mg.l−1 + NAA 0.5 mg.l−1, P15 = TDZ 1.0 mg.l−1 + NAA 1.0 mg.l-1. The percentage of callus induction observed, percentage of shoot induction, percentage of browning, percentage of contamination and percentage of live explants at 28 DAI. The results showed that the percentage of explants forming callus was 56.4%, and the percentage of explants forming organ was 54.6%. In these two parameters the treatment of P14 and P15 gave the best results of 6.6% respectively. The percentage of explants experiencing browning was 26.1%, treatment P1 gave the highest response for this parameter, namely 6.6%. The parameter percentage of live explants was 71.3%. The best response was found in treatment P2, P3, P12 and P15, which were 6.6% each. The fastest bud formation occurred at 14 Days After Initiation (DAI) in P4 treatment. The resulting shoots look quite vigor and have formed leaves. In the combination treatment P14, showed the best results, the addition of auxin was able to increase the initiation of embryogenic callus.

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Section: Percentage Of Callus Induction and Callus Appearancesupporting

confidence: 88%

Indirect organogenesis of Aceh patchouli leaf explants (Pogostemon cablin Benth) by in vitro

Puspita

Efendi

Zakaria

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…The growth regulator was effective in the low concentration, with a too high concentration that would inhibit cell division. This is supported by Hariyati et al, (2016) that, the higher BAP concentration will inhibit the callus formation in the chrysanthemum plant. In line with Kieber and Schaller (2014) in plant tissues contain cytokinin oxidase enzymes.…”

Section: Resultsmentioning

confidence: 74%

“…The colour of the yellowish-green callus shows the callus in good condition. According to Hariyati et al, (2016), the callus color was one indicator of callus quality. The green callus shows the formation of chlorophyll.…”

Section: Resultsmentioning

confidence: 99%

Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine

Aprilia

Setiari²,

Nurchayati³

2022

J Bio Bio Edu

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Potato has the potential for food diversification.The propagation method in a short period is needed. One of the methods used is plant tissue culture. Benzyl Amino Purine (BAP), determine the success of propagation by tissue culture method. This research aimed to study the use of different BAP concentrations for callus development from stem explants. The explants put on culture medium added by 0, 1, 2, 3 ppm BAP. The development of the stem explant observed every week for a month. The results showed that callus formed in all media. Without BAP treatment, callus were formed after 2 weeks and got browning, then stopped growing. Callus grew and showed differentiation by application of all the BAP concentration. Callus greowth was optimally at 2 ppm BAP treatment. The callus from 1 ppm BAP produced the most number of roots, shoots and leaves than another concentration. This experiment showed that different BAP concentrations affected callus development of S. tuberosum from stem explant. The conclusion was callus growth has obtained by the treatment of 2 ppm BAP, while the development of callus has obtained on addition of 1 ppm BAP. The novelty of this research is the callus induction method from potato sprout stems grown from potato seeds with plant growth regulators Benzyl Amino Purine. Callus induction method from potato sprout stems grown from potato seeds can be a guide for embryogenic callus induction.

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“…Kalus remah memiliki ciriciri ruang antarselnya terlihat renggang dan mudah dipisahkan. Tekstur kalus dipengaruhi oleh pemberian zat pengatur tumbuh, jenis eksplan, dan kondisi lingkungan kultur (Hariyati et al, 2016;Sugiyarto & Kuswandi, 2014). Pemberian kombinasi zat pengatur tumbuh 2,4-D dan kinetin pada kalus memicu aktifnya pembelahan dan pembesaran sel sehingga terbentuk kalus bertekstur remah.…”

Section: Hasil Dan Pembahasanunclassified

Pengaruh Pemberian Elisitor Ekstrak Khamir pada Pertumbuhan Kultur Kalus Gembili dengan Penambahan ZPT 2,4-D dan Kinetin

Lutfiah

Habibah

2022

ijmns

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Senyawa bioaktif pada tanaman gembili dapat dihasilkan lebih banyak dari kalus dibandingkan tanaman utuhnya. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian 2,4-D dan kinetin serta elisitor ekstrak khamir terhadap pertumbuhkan kultur kalus gembili. Rancangan penelitian yang digunakan adalah rancangan acak lengkap dua faktorial, yaitu kombinasi 2,4-D (1 ppm; 0,5 ppm) dan kinetin (1 ppm; 0,5 ppm) serta elisitor ekstrak khamir (0,5%; 1%; 2,5% (b/v)). Kalus berumur tiga bulan disubkultur dan diinkubasi selama 2,5 bulan. Kemudian dielisitasi dengan ekstrak khamir dan dipelihara selama 30 hari. Indikator pertumbuhan kalus yang diamati meliputi berat basah, warna dan tekstur kalus. Data dianalisis menggunakan ANOVA dua arah dan diuji lanjut dengan DMRT 5%. Hasil penelitian menunjukkan bahwa kombinasi 2,4-D dan kinetin berpengaruh terhadap rerata berat basah kalus. Sedangkan pemberian elisitor ekstrak khamir tidak berpengaruh terhadap rerata berat basah kalus gembili. Rerata berat basah kalus tertinggi sebesar 3,35 gram dicapai pada kombinasi 2,4-D 0,5 ppm + kinetin 0,5 ppm dengan elisitor 1% (b/v). Tekstur kalus yang dihasilkan pada seluruh perlakuan adalah remah dan berwarna coklat muda hingga coklat kehitaman.

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INDUKSI KALUS TANAMAN KRISAN (Chrysanthemum morifolium) DENGAN PEMBERIAN BENZIL AMINO PURIN (BAP) DAN DICHLOROFENOKSI ACETIL ACID (2,4 D)

Cited by 7 publications

References 0 publications

Indirect organogenesis of Aceh patchouli leaf explants (Pogostemon cablin Benth) by in vitro

Indirect organogenesis of Aceh patchouli leaf explants (Pogostemon cablin Benth) by in vitro

Callus Development from Potato (Solanum tuberosum) Stem at Various Concentrations of Benzylaminopurine

Pengaruh Pemberian Elisitor Ekstrak Khamir pada Pertumbuhan Kultur Kalus Gembili dengan Penambahan ZPT 2,4-D dan Kinetin

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