Inductive effect produced by a mixture of carbon source in the production of gibberellic acid by Gibberella fujikuroi

Rios‐Iribe, Erika Y.; Flores-Cotera, Luis B.; Chavira, Mario Martín González; González‐Alatorre, Guillermo; Escamilla‐Silva, Eleazar M.

doi:10.1007/s11274-010-0603-4

Cited by 26 publications

(16 citation statements)

References 22 publications

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“…It was observed that GA production initiated on the first day of fermentation, which is contrary to reports that GA commences about 46 h after the initiation of fermentation following nitrogen depletion in the medium (Escamilla et al, 2000;Rodrigues et al, 2009;Rios-Iribe et al, 2011). However, Lale and Gadre (2010) also reported GA production at 20 h by a mutant of G. fujikuroi.…”

Section: Resultscontrasting

confidence: 72%

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Omojasola

Adejoro

2018

Not Sci Biol

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The present study aimed to produce gibberellic acid through fermentation using banana (Musa sapientum) peel waste as substrate. Banana peel, a domestic and industrial waste, constitutes a potential source of cheap fermentable substrate for the production of other value-added products. Fusarium moniliforme ATCC 10052 and Aspergillus niger CBS 513.88 were used as fermenting organisms. The substrate was dried, ground and its proximate composition determined. The powdered substrate was added to a modified CzapekDox broth (a semisynthetic medium), with Carboxyl methylcellulose (CMC) as control. The fermentation conditions were: pH 5.5; inoculum size 1% (5 × 10 5 spores/mL F. moniliforme) (2 × 10 6 spores/mL A. niger); substrate concentration 2 g; temperature 25 ± 2 o C; fermentation time 7 days. The fermentation was optimized by varying pH, inoculum size, substrate concentration and fermentation time. The extracted GA was subjected to infra-red spectroscopy using FT-IR. The parameters which gave the highest GA yields were thereafter combined in a single fermentation. The results of proximate analysis of banana peel substrate revealed 8.65% moisture, 9.54% protein, 5.40% lipids, 11.45% ash, 22.34% crude fibre, and 42.62% carbohydrate. The GA yields of 13.55 g/L and 12.44 g/L were produced from the banana peel substrate and 3.62 and 2.61 g/L from the CMC control by F. moniliforme and A. niger respectively. Under optimized conditions, F. moniliforme produced 17.48 g/L GA, while A. niger produced 13.50 g/L. Extracted GA was similar to standard GA sample and the present results support the potential use of banana peel for fermentative GA production.

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Section: Resultscontrasting

confidence: 72%

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Omojasola

Adejoro

2018

Not Sci Biol

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“…Meanwhile, the root lengths of three osipms1 mutants were significantly increased by amino acids treatment compared to those in untreated seeds (Figure b–e). Ketogenic amino acid, for example Leu, Ile, and Phe, can be degraded directly into acetyl‐CoA for GA biosynthesis (Rios‐Iribe et al ., ). Here, we predicted that amino acid treatment contributing to seed vigour of osipms1 mutants might be through stimulating GA biosynthesis during seed germination.…”

Section: Resultsmentioning

confidence: 97%

Influence of isopropylmalate synthase OsIPMS1 on seed vigour associated with amino acid and energy metabolism in rice

Jiaan

et al. 2018

Plant Biotechnology Journal

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Seed vigour is an imperative trait for the direct seeding of rice. Isopropylmalate synthase (IPMS) catalyses the committed step of leucine (Leu) biosynthesis, but its effect on seed vigour remains unclear. In this study, rice OsIPMS1 and OsIPMS2 was cloned, and the roles of OsIPMS1 in seed vigour were mainly investigated. OsIPMS1 and OsIPMS2 catalyse Leu biosynthesis, and Leu feedback inhibits their IPMS activities. Disruption of OsIPMS1 resulted in low seed vigour under various conditions, which might be tightly associated with the reduction of amino acids in germinating seeds. Eleven amino acids that associated with stress tolerance, GA biosynthesis and tricarboxylic acid (TCA) cycle were significantly reduced in osipms1 mutants compared with those in wide type (WT) during seed germination. Transcriptome analysis indicated that a total of 1209 differentially expressed genes (DEGs) were altered in osipms1a mutant compared with WT at the early germination stage, wherein most of the genes were involved in glycolysis/gluconeogenesis, protein processing, pyruvate, carbon, fructose and mannose metabolism. Further analysis confirmed that the regulation of OsIPMS1 in seed vigour involved in starch hydrolysis, glycolytic activity and energy levels in germinating seeds. The effects of seed priming were tightly associated with the mRNA levels of OsIPMS1 in priming seeds. The OsIPMS1 might be used as a biomarker to determine the best stop time-point of seed priming in rice. This study provides novel insights into the function of OsIPMS1 on seed vigour and should have practical applications in seed priming of rice.

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“…There are reports that the production of phytohormones, like that of several other secondary metabolites, is initiated by the exhaustion of nitrogen (Shukla (Fox & Howlett 2008;Rios-Iribe et al 2011) sources from the medium (Brückner 1992;Tudzynski 1999). Maximal IAA, GA and ethylene production by F. culmorum strains was observed in 96-h cultures, when the sources of carbon and nitrogen in the culture (results not shown) were largely depleted.…”

Section: Discussionmentioning

confidence: 90%

Efficiency of indoleacetic acid, gibberellic acid and ethylene synthesized in vitro by Fusarium culmorum strains with different effects on cereal growth

2014

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Three different Fusarium culmorum strains having a pathogenic, a deleterious (deleterious rhizosphere microorganism), or a promoting (plant growth promoting fungus) effect on plant growth were studied for their ability to synthesize in vitro the phytohormones indoleacetic acid (IAA), gibberellic acid (GA), and ethylene. All the phytohormones tested were synthesized in cultures supplemented with wide concentration ranges of glucose and tryptophan or methionine (precursors of phytohormone synthesis). The amounts of these secondary metabolites synthesized by the particular strains were found to be significantly different. The non-pathogenic PGPF strain (DEMFc2) synthesized the highest amounts of IAA and GA, a fact that could be responsible for the growth-promoting properties of this strain. A pathogenic strain synthesized the highest amount of ethylene, which could be responsible for the negative effect of this strain on plant growth. F. culmorum isolates with a high capacity for IAA synthesis also have a high capacity for GA synthesis and irrespective of the growth conditions, a high positive correlation (R > 0.9) between the concentrations of synthesized IAA and GA in F. culmorum cultures was found. It is worth mentioning that the optimal conditions for the growth of F. culmorum isolates and the synthesis of the individual phytohormones differed from one another. The optimal growth conditions were 1.0% of glucose and 9.9 mM of methionine or 6.0 mM of tryptophan. The optimal conditions for ethylene synthesis were 0.5% of glucose and 6.6 mM of methionine, whereas 1.0% of glucose and 9.0 mM of tryptophan were optimal for IAA and GA synthesis.

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Inductive effect produced by a mixture of carbon source in the production of gibberellic acid by Gibberella fujikuroi

Cited by 26 publications

References 22 publications

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Gibberellic Acid Production by Fusarium moniliforme and Aspergillus niger Using Submerged Fermentation of Banana Peel

Influence of isopropylmalate synthase OsIPMS1 on seed vigour associated with amino acid and energy metabolism in rice

Efficiency of indoleacetic acid, gibberellic acid and ethylene synthesized in vitro by Fusarium culmorum strains with different effects on cereal growth

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