Inductive and synergistic interactions between plant allelochemical flavone and Bt toxin Cry1Ac in <i>Helicoverpa armigera</i>

Wang, Shan; Zhang, Min; Huang, Jinyong; Li, Leyao; Huang, Kejie; Zhang, Yuting; Li, Yalu; Deng, Zhongyuan; Ni, Xinzhi; Li, Xianchun

doi:10.1111/1744-7917.12897

Cited by 9 publications

(8 citation statements)

References 50 publications

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“…Plant secondary metabolites like phenols, alkaloids, flavonoids, and terpenes have been shown to impede the growth and population development of insects [ 2 , 3 , 4 ]. These toxic metabolites are difficult to digest or are neurotoxic to insects [ 2 , 5 ]. In response, insects have evolved diverse strategies, including detoxification enzymes, such as cytochrome P450 (P450s) monooxygenases, carboxylesterases, and glutathione S-transferases (GSTs), to adapt to different host plants and detoxify phyto-toxins [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug Riptortus pedestris to Different Food Resources

Zhang,

Wang,

et al. 2023

Insects

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The bean bug, Riptortus pedestris (Hemiptera: Heteroptera), poses a significant threat to soybean production, resulting in substantial crop losses. Throughout the soybean cultivation period, these insects probe and suck on various parts of plants, including leaves, pods, and beans. However, the specific mechanisms by which they adapt to different food resources remain unknown. In this study, we conducted gut transcriptomic analyses of R. pedestris fed with soybean leaves, pods, and beans. A total of 798, 690, and 548 differently expressed genes (DEGs) were monitored in G—pod vs. G—leaf (comparison of insect feeding on pods and leaves), G—bean vs. G—leaf (comparison of insect feeding on beans and leaves), and G—pod vs. G—bean (comparison of insect feeding on pods and beans), respectively. When fed on pods and beans, there was a significant increase in the expression of digestive enzymes, particularly cathepsins, serine proteases, and lipases. Conversely, when soybean leaves were consumed, detoxification enzymes, such as ABC transporters and 4-coumarate-CoA ligase, exhibited higher expression. Our findings indicate that R. pedestris dynamically regulates different metabolic pathways to cope with varying food resources, which may contribute to the development of effective strategies for managing this pest.

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Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug Riptortus pedestris to Different Food Resources

Zhang,

Wang,

et al. 2023

Insects

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“…Among the reported synergistic cases are azadirachtin + Bacillus thuringiensis Berliner sub sp. kurstaki against H. armigera [32], maize insect resistance cysteine protease (Mir1-CP) + Cry2A against Helicoverpa zea, H. virescens, Spodoptera frugiperda, and Diatraea grandiosella [33], gossypol + Cry1Ac against a resistant strain of H. zea [34], jasmonic acidinduced resistance plus Cry1Ac or Cry1Ac + Cry2Ab against S. frugiperda [35], and flavone + Cry1Ac against H. armigera [36]. The antagonistic cases are tannis + Cry1Ac against H. armigera [37], quercetin + Cry1Ac against H. armigera [38], and Bt + trichlorfon against Plutella xylostella [39].…”

Section: Introductionmentioning

confidence: 99%

Sequential and Simultaneous Interactions of Plant Allelochemical Flavone, Bt Toxin Vip3A and Insecticide Emamectin Benzoate in <em>Spodoptera frugiperda</em>

Huang¹,

He²,

Wang³

et al. 2023

Preprint

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Target pests of genetically engineered crops producing both defensive allelochemicals and Bacillus thuringiensis (Bt) toxins often sequentially or simultaneously uptake allelochemicals, Bt toxins, and/or insecticides. How the three types of toxins interact to kill pests remains underexplored. Here we investigated the interactions of Bt toxin Vip3A, plant allelochemical flavone, and insecticide emamectin benzoate in Spodoptera frugiperda. Simultaneous administration of flavone LC25 + Vip3A LC25, emamectin benzoate LC25 + Vip3A LC25, and flavone LC15 + emamectin benzoate LC15 + Vip3A LC15 but not flavone LC25 + emamectin LC25 yielded a mortality significantly higher than their expected additive mortality (EAM). One-day preexposure to one toxin at LC5 followed by 6-day exposure to the same toxin at LC5 plus another toxin at LC50 showed that the mortality of flavone LC5 + Vip3A LC50, emamectin benzoate LC5 + Vip3A LC50, and Vip3A LC5 + emamectin benzoate LC50, were significantly higher than their EAM, while that of flavone LC5 + emamectin benzoate LC50 was significantly lower than their EAM. No significant difference existed among the mortalities of Vip3A LC5 + flavone LC50, emamectin benzoate LC5 + flavone LC50, and their EAMs. The results suggest that the interactions of the three toxins are largely synergistic (inductive) or additive, depending on their combinations and doses.

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“…Among the reported synergistic cases are azadirachtin + Bacillus thuringiensis Berliner sub sp. kurstaki against H. armigera [ 32 ], maize insect resistance cysteine protease (Mir1-CP) + Cry2A against Helicoverpa zea , H. virescens , Spodoptera frugiperda, and Diatraea grandiosella [ 33 ], gossypol + Cry1Ac against a resistant strain of H. zea [ 34 ], jasmonic acid-induced resistance plus Cry1Ac or Cry1Ac + Cry2Ab against S. frugiperda [ 35 ], and flavone + Cry1Ac against H. armigera [ 36 ]. The antagonistic cases are tannis + Cry1Ac against H. armigera [ 37 ], quercetin + Cry1Ac against H. armigera [ 38 ], and Bt + trichlorfon against Plutella xylostella [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

“…The antagonistic cases are tannis + Cry1Ac against H. armigera [ 37 ], quercetin + Cry1Ac against H. armigera [ 38 ], and Bt + trichlorfon against Plutella xylostella [ 39 ]. So far, there has been only one sequential ingestion case study, which showed that pre-exposure to Cry1Ac significantly induced flavone’s toxicity against H. armigera , whereas pre-exposure to flavone did not induce or inhibit Cry1A’s toxicity against the same pest species [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Sequential and Simultaneous Interactions of Plant Allelochemical Flavone, Bt Toxin Vip3A, and Insecticide Emamectin Benzoate in Spodoptera frugiperda

Huang,

He,

Wang

et al. 2023

Insects

Self Cite

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Target pests of genetically engineered crops producing both defensive allelochemicals and Bacillus thuringiensis (Bt) toxins often sequentially or simultaneously uptake allelochemicals, Bt toxins, and/or insecticides. How the three types of toxins interact to kill pests remains underexplored. Here we investigated the interactions of Bt toxin Vip3A, plant allelochemical flavone, and insecticide emamectin benzoate in Spodoptera frugiperda. Simultaneous administration of flavone LC25 + Vip3A LC25, emamectin benzoate LC25 + Vip3A LC25, and flavone LC15 + emamectin benzoate LC15 + Vip3A LC15 but not flavone LC25 + emamectin LC25 yielded a mortality significantly higher than their expected additive mortality (EAM). One-day pre-exposure to one toxin at LC5 followed by six-day exposure to the same toxin at LC5 plus another toxin at LC50 showed that the mortality of flavone LC5 + Vip3A LC50, emamectin benzoate LC5 + Vip3A LC50, and Vip3A LC5 + emamectin benzoate LC50 were significantly higher than their EAM, while that of flavone LC5 + emamectin benzoate LC50 was significantly lower than their EAM. No significant difference existed among the mortalities of Vip3A LC5 + flavone LC50, emamectin benzoate LC5 + flavone LC50, and their EAMs. The results suggest that the interactions of the three toxins are largely synergistic (inductive) or additive, depending on their combinations and doses.

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Inductive and synergistic interactions between plant allelochemical flavone and Bt toxin Cry1Ac in Helicoverpa armigera

Cited by 9 publications

References 50 publications

Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug Riptortus pedestris to Different Food Resources

Comparative Transcriptomic Analysis Reveals Adaptation Mechanisms of Bean Bug Riptortus pedestris to Different Food Resources

Sequential and Simultaneous Interactions of Plant Allelochemical Flavone, Bt Toxin Vip3A and Insecticide Emamectin Benzoate in <em>Spodoptera frugiperda</em>

Sequential and Simultaneous Interactions of Plant Allelochemical Flavone, Bt Toxin Vip3A, and Insecticide Emamectin Benzoate in Spodoptera frugiperda

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