Induction, subculture cycle, and regeneration of callus in Safed musli (Chlorophytum borivilianum) using different types of phytohormones

Abstract

Background:Chlorophytum borivilianum is an industrially valued medicinal crop. Propagation through seeds is not feasible because of low germination percentage and long dormancy period. Therefore, callus culture and plant regeneration can be an alternative to improve this crop production. Also, callus can serve as an alternative source of bioactive compounds.Objective:To evaluate the effect of different phytohormones on callus induction, subculture cycle, and regeneration studies of callus in C. borivilianum.Materials and Methods:Young shoot buds of C. borivilianum were inoculated on Murashige and Skoog medium fortified with 3% sucrose and different concentrations (0, 1, 5, 10, and 15 mg/L) of either naphthalene acetic acid or 2,4-dichlorophenoxyacetic acid or indole-3-acetic acid and callus induction was evaluated up to four subcultures cycles. Shoot regeneration from callus was studied on Murashige and Skoog media fortified with 6-benzylaminopurine andkinetin or thidiazuron at varied levels (0, 0.5, 1, 2, and 3 mg/L). Microshoots were rooted on Murashige and Skoog media supplemented with 1.0 mg/L indole-3-butyric acid and plantlets were acclimatized before transferred to the natural conditions.Results:Callus induction was better evidenced on Murashige and Skoog media containing 5 mg/L 2,4-dichlorophenoxyacetic acid up to fourth subculture. Callus differentiated into shoots on Murashige and Skoog media fortified with 6-benzylaminopurine or kinetin, whereas thidiazuron completely failed to regenerate shoots. Furthermore, microshoots rooted on 1.0 mg/L indole-3-butyric acid containing Murashige and Skoog media. The rooted plantlets were successfully acclimatized and established in soil with 88.3% survivability.Conclusion:The type of auxins played an important role in inducing callus tissue from shoot bud explants of Safed musli. In future, this in vitro protocol could benefit in crop improvement programs and serve as a new source of bioactive compounds from Safed musli callus tissue for various therapeutic applications.SUMMARY Explants de-differentiated to form callus on Murashige and Skoog media containing 5 mg/L 2,4-D up to fourth subculture.Callus re-differentiated into shoots on Murashige and Skoog media fortified with 0.5 mg/L BAP.In vitro rooting of shoots was achieved on 1.0 mg/L IBA containing Murashige and Skoog media.The rooted plantlets were successfully acclimatized and established in soil with 88.3% survivability. Abbreviations used: MS: Murashige and Skoog, NAA: naphthalene acetic acid, 2,4-D: 2,4-dichlorophenoxyacetic acid, IAA: indole-3-acetic acid, BAP: 6-benzylaminopurine, Kn: Kinetin, TDZ: thidiazuron, IBA: indole-3-butyric acid, RCBD: Randomized Complete Block Design, DMRT: Duncan's Multiple Range Test

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

“…This factor becomes crucial to assess the genetical stability of in vitro-cultured plants, making their valorization easier. Moreover, long-term subcultures constitute an efficient strategy to improve interesting phenomena under in vitro conditions, such as rooting (Mendonça et al, 2019;Wang and Yao, 2020), plant regeneration (Konar et al, 2019) and callus induction (Nakasha et al, 2016), very useful for biotechnological production of by-products from medicinal plants.…”

In vitro propagation of pharmaceutical and endangered medicinal plants- a mini review

“…Callus growth was better exhibited on MS media having 5 mg/l 2, 4-D. Differentiation of callus into shoots was noted on MS media with BAP or kinetin, whereas TDZ was unable to regrow any shoots. The rooted plantlets were effectively acclimatized and grown in soil resulting in 88.3% survivability (Nakasha et al, 2016). Mun and Mun (2016) reported an efficient clonal mass growth of Rheum coreanum Nakai, a rare herb in Korea using rhizome in vitro culture.…”

Origanum vulgare L. Cuttings Rhizogenesis in Microclonal Reproduction in Vitro

“…і лікарських [20], застосовується як для оптимізації біотехнологій мікроклонального розмноження цінних видів рослин, так і з використанням калюсних культур, культури пиляків тощо для біотехнологічного забезпечення селекційного процесу [21,22]. Утім дослідники наголошують на диференційованій генотиповій реакції за рахунок внутрішньовидової спадкової мінливості на варіювання як фітогормонального складу живильних середовищ, так і взагалі умов культивування [23,24].…”