Induction Specificity and Catabolite Repression of the Early Enzymes in Camphor Degradation by Pseudomonas putida

Abstract

The ability of bornane and substituted bornanes to induce the early enzymes for D(+)-camphor degradation and control of these enzymes by catabolite repression were studied in a strain of a Pseudomonas putida. Bornane and 20 substituted bornane compounds showed induction. Of these 21 compounds, bornane and 8 of the substituted bornanes provided induction without supporting growth. Oxygen, but not nitrogen, enhanced the inductive potency of the unsubstituted bornane ring. All bornanedione isomers caused induction, and those with substituents on each of the three consecutive carbon atoms, including the methyl group at the bridgehead carbon, showed induction without supporting growth. Although it was not possible to obtain experimental data for a case of absolute gratuitous induction by compounds not supporting growth, indirect evidence in support of gratuitous induction is presented. It is proposed that the ability of P. putida to tolerate the unusually high degree of possible gratuitous induction observed for camphor catabolism may be related to the infrequent occurrence of bicyclic ring structures in nature. Survival of an organism with a broad specificity for gratuitous induction is discussed. Glucose and succinate, but not glutamate, produced catabolite repression of the early camphor-degrading enzymes. Pathway enzymes differ in their degree of sensitivity to succinate-provoked catabolite repression. The ability of a compound to produce catabolite repression is not, however, directly related to the duration of the lag period (diauxic lag) between growth on camphor and growth on the repressing compound.

Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWW0 under various conditions of nutrient limitation in chemostat culture

“…This phenomenon, which results in batch culture in the sequential utilization of the carbon sources (diauxie), is generally referred to as catabolite repression (18). The expression of catabolic pathways for camphor (11), protocatechuate (35), benzene (22), styrene (25), and aniline (13) was reported earlier to be subject to catabolite repression. The most detailed studies on aromatic compounds have been performed on Pseudomonas putida mt-2 harboring the TOL plasmid pWW0 that encodes the degradation of toluene, m-and p-xylene, pseudocumene, and m-ethyltoluene (3) ( Fig.…”

The P alkBFGHJKL Promoter Is under Carbon Catabolite Repression Control in Pseudomonas oleovorans but Not in Escherichia coli alk + Recombinants

“…In Pseudomonas species, the preferred carbon source is usually an organic acid such as lactate or a tricarboxylic acid cycle intermediate like citrate or succinate (29). In the presence of these substrates, the expression of catabolic pathways for terpenes such as camphor (24); for aromatic compounds like benzene (31), styrene (37), aniline (25), protocatechuate (47), phenol (34), and toluene (13,26,30); for chloroaromatic compounds (32); and for other nonaromatic compounds and carbohydrates (29) is subject to carbon catabolite repression.…”

Biocatalytic Synthesis of a Diketobornane as a Building Block for Bifunctional Camphor Derivatives

“…The well‐characterized camphor degradation pathway from P. putida was used as a starting point for the regio‐ and stereoselective CH bond activation of camphor 17. The first step in this pathway is the selective hydroxylation of camphor at position 5, leading to 2 by one of the best studied monooxygenase systems P450cam (Scheme ) 18.…”