Induction, regeneration and characterization of tetraploids and variants in ‘Tapestry’ caladium

Cai, Xiaodong; Cao, Zhe; Xu, Shixiao; Deng, Zhanao

doi:10.1007/s11240-014-0636-8

Cited by 31 publications

(44 citation statements)

References 28 publications

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“…Chromosome numbers were determined as described by Cao et al (2014), by squashing root tip cells, staining them with aceto-carmine, and photographing at 91000 magnification percentage of aneuploid variants reached 62.5 %. This percentage was 77.1 % higher than the rate of aneuploids (35.7 %) in 'Tapestry' caladium plants regenerated from colchicine-treated leaf explants (Cai et al 2015). The 'Tapestry' leaf explants used in the previous study (Cai et al 2015) were from immature, furled leaves cultured on a medium containing NAA.…”

Section: Discussionmentioning

confidence: 82%

“…This percentage was 77.1 % higher than the rate of aneuploids (35.7 %) in 'Tapestry' caladium plants regenerated from colchicine-treated leaf explants (Cai et al 2015). The 'Tapestry' leaf explants used in the previous study (Cai et al 2015) were from immature, furled leaves cultured on a medium containing NAA. These conditions (immature leaf explants ?…”

Section: Discussionmentioning

confidence: 82%

“…Observed variations include changes in leaf shape, apex, base and margin, color of lamina, spotting, veins, and petiole attachment and color (Ahmed et al 2002(Ahmed et al , 2004Thepsithar et al 2010). In a previous study, we observed a number of caladium aneuploids, which prompted us to suspect that chromosome number changes might be part of the genetic causes of the observed variations (Cai et al 2015).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 88%

“…Mean nuclear contents followed by different letters were statistically different according to T test with the Bonferroni adjusted P value = 0.0002. Nuclear DNA content changes in the somaclonal variants were calculated as described by Cai et al (2015), by assuming 100 % in the wildtype and then subtracting 100 from the percent DNA content increase (?) or decrease (-) of the variants.…”

Section: Discussionmentioning

confidence: 99%

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Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Cao

Sui

Cai

et al. 2016

Plant Cell Tiss Organ Cult

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Somaclonal variation is a major concern to many applications of plant cell and tissue culture techniques, yet it could be a useful source of variation for plant genetic improvement. High frequencies of somaclonal variants were reported in caladium, but little was known about changes in caladium somaclonal variants at the cellular and molecular levels. Twenty-four somaclonal variants were identified among 'Red Flash' caladium plants regenerated from two types of leaf explants and on two media containing different auxins. Results showed that the type of leaf explants and auxin affected the occurrence of somaclonal variants in 'Red Flash'. The highest percentage of variants (25.0 %) was observed among plants regenerated from mature leaf explants cultured on the media containing 2,4-dichlorophenoxyacetic acid. These somaclonal variants exhibited considerable changes in leaf shape, coloring of the main veins, spots, margins, and leaf size, and could be separated into 10 somaclonal variant groups. Twelve variants contained 1.1-5.4 % less nuclear DNA and appeared to have lost one chromosome. Two variants contained 5.4-9.2 % less nuclear DNA and appeared to have lost two chromosomes. One variant contained 95.0 % more nuclear DNA and 2n = 58 chromosomes. Two SSR markers (CaM1 and CaM103) revealed DNA banding pattern changes in nine variants, including allele loss in eight variants and allele size change in one variant. These results suggested that several cytological and/or molecular causes were involved in the somaclonal variation in 'Red Flash' and chromosome number change was a frequent cause leading to a high frequency of aneuploids and marker allele loss. The CaM1-carrying chromosome seemed to be unstable and prone to loss during tissue culture.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 82%

Section: Electronic Supplementary Materialsmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

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Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Cao

Sui

Cai

et al. 2016

Plant Cell Tiss Organ Cult

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“…Caladium (Caladium × hortulanum Birdsey) is an important ornamental plant valued for its long-lasting colorful foliage, and is commonly grown in containers and in the landscape (Deng 2018(Deng , 2012. A great deal of reports indicated that caladium could be propagated by in vitro culturing of leaf segments, meristematic tissues or petiole segments (Ahmed et al 2004;Thongpukdee et al 2010), and morphological variants were frequently observed among these regenerated plants (Cai et al 2015;Cao et al 2016). Recent studies found that chromosome gain or loss was a common type of cytological variation in caladium (Cai et al 2015;Cao et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Effects of Different Nutrient Solutions on the Acclimatisation of in vitro Caladium Plantlets using a Simplified Hydroponic System

Zhang¹,

Gu²,

Chen³

et al. 2019

JSM

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An efficient acclimatisation protocol is critical to improve the survival rate and promote growth of in vitro plantlets. Herein, tissue-cultured plantlets of 'Red Flash' caladium (Caladium × hortulanum Birdsey) were treated with deionised water and three nutrient solutions including Japanese garden formula (JG), South China Agriculture University formula B for leafy vegetables (SCAU-B) and Hoagland solution in a simplified hydroponic system under indoor environmental conditions. The results showed that all the plantlets survived after 30 days of hydroponic culture, and the three nutrient solutions showed significant positive effects on the plant growth and the content of chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, soluble sugar and soluble protein compared to the deionised water treatment. No significant difference was recorded for the other growth parameters except the average leaf number per plantlet among the nutrient solution treatments. For the analysed physiological parameters, the plantlets treated by SCAU-B contained significantly higher content than those of the Hoagland treatment except the protein content. No significant difference was found between the JG and the SCAU-B treatment except the carotenoid content, and between the JG and the Hoagland treatment except the protein content. The plantlets acclimatised in the nutrient solutions also had a good performance when transferred to plastic pots. These results implied that water alone was not capable of maintaining healthy growth of in vitro caladium plantlets, and hydroponics approach using the three nutrient solutions could be a feasible method for acclimatisation of the plantlets.

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Caladium

Deng

2018

Handbook of Plant Breeding

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Induction, regeneration and characterization of tetraploids and variants in ‘Tapestry’ caladium

Cited by 31 publications

References 28 publications

Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Somaclonal variation in ‘Red Flash’ caladium: morphological, cytological and molecular characterization

Effects of Different Nutrient Solutions on the Acclimatisation of in vitro Caladium Plantlets using a Simplified Hydroponic System

Caladium

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