Induction of zymosan‐air‐pouch inflammation in rats and its characterization with reference to the effects of anticomplementary and anti‐inflammatory agents

Konno, Satoshi; Tsurufuji, Susumu

doi:10.1111/j.1476-5381.1983.tb10030.x

Cited by 51 publications

(32 citation statements)

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“…7 Thereafter came a period when it was recognized that this model is useful for the study of immune reactions, bone and cartilage breakdown, and also for drug testing. [8][9][10][11][12][13] In addition to cell infiltrations, the production of biological agents, eg, matrix metalloproteinases (MMPs), was studied in the pouch exudates.…”

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confidence: 99%

Intradermal air pouch leukocytosis as an in vivo test for nanoparticles

Vandooren¹,

Berghmans²,

Dillen³

et al. 2013

IJN

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The need for test systems for nanoparticle biocompatibility, toxicity, and inflammatory or adaptive immunological responses is paramount. Nanoparticles should be free of microbiological and chemical contaminants, and devoid of toxicity. Nevertheless, in the absence of contamination, these particles may still induce undesired immunological effects in vivo, such as enhanced autoimmunity, hypersensitivity reactions, and fibrosis. Here we show that artificial particles of specific sizes affect immune cell recruitment as tested in a dermal air pouch model in mice. In addition, we demonstrate that the composition of nanoparticles may influence immune cell recruitment in vivo. Aside from biophysical characterizations in terms of hydrodynamic diameter, zeta potential, concentration, and atomic concentration of metals, we show that – after first-line in vitro assays – characterization of cellular and molecular effects by dermal air pouch analysis is straightforward and should be included in the quality control of nanoparticles. We demonstrate this for innate immunological effects such as neutrophil recruitment and the production of immune-modulating matrix metalloproteases such as MMP-9; we propose the use of air pouch leukocytosis analysis as a future standard assay.

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Intradermal air pouch leukocytosis as an in vivo test for nanoparticles

Vandooren¹,

Berghmans²,

Dillen³

et al. 2013

IJN

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“…Moreover, zymosan caused the formation of an apparently aggregated layer of PMNL onto the inner surface of the pouch wall. Generation of proinflammatory frag ments, C3a and C5a, of complement via activation by zymosan of the alternative pathway of the complement system was assumed to play an important role in the mechanism of induction of this inflammatory response, since the anticomplementary agent K-76COONa was effective in inhibiting leukocyte accumulation in the inflammatory site (1 ). The present experiments were undertaken to gain further insight into mechanisms of zymosan-induced inflam mation.…”

Section: Discussionmentioning

confidence: 99%

“…Activation of the alternative pathway of the complement system by zymosan has been assumed to be involved in the pathogenesis of the zymosan-air-pouch inflammation (1 ).…”

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confidence: 99%

“…Consequently, the degranulation is generally accompanied with elevation of vascular permeability in the local tissues (11)(12)(13). Induction of zymosan-air-pouch inflam mation: The zymosan-air-pouch inflammation was induced as described (1). In brief, rats were injected with 8.0 ml of air subcu taneously on the back under light ether anaesthesia to make an ellipsoid or oval air pouch.…”

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Analysis of the Factor(s) Involved in Pathogenesis of Zymosan-Induced Inflammation in Rats

Konno

Tsurufuji

1985

Japanese Journal of Pharmacology

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“…In the air pouch model, injection of zymosan into pouches formed by subcutaneous injection of air in the back of the mice induces the accumulation of neutrophils [22]. Similarly, neutrophils accumulate in the peritoneal cavity 4 h after thioglycolate injection [23].…”

Section: Down-regulation Of Cd69 Affects Inflammatory Mediators In Caiamentioning

confidence: 99%

The role of CD69 in acute neutrophil‐mediated inflammation

et al. 2006

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The leukocyte activation marker CD69 functions as a negative regulator of the immune response, both in NK-dependent tumor rejection and in the inflammation associated with lymphocyte-dependent collagen-induced arthritis. In contrast, it has been reported that CD69-deficient mice are refractory to the neutrophil-dependent acute inflammatory response associated with anti-type II collagen antibody-induced arthritis (CAIA), suggesting a positive regulatory role for CD69 in neutrophil function during arthritis induction. To clarify this discrepancy, the CAIA response was independently analyzed in our CD69-deficient mice. In these experiments, the inflammatory response was unaffected by CD69 deficiency. Additionally, the in vivo down-regulation of CD69 expression by treatment of wild-type mice with the anti-CD69 mAb 2.2, which mimics the CD69-deficient phenotype, did not affect the course of arthritis in this model. Moreover, down-regulation of CD69 expression increased expression in arthritic joints of key inflammatory mediators, including IL-1b, IL-6 and the chemokine MCP-1. Neutrophil accumulation in zymosan-treated air pouches and in thioglycolate-treated peritoneal cavities was also unaffected in CD69-deficient mice. In addition, CD69 expression was absent in activated neutrophils. Taken together, these results rule out a significant stimulatory role for CD69 in acute inflammatory responses mediated by neutrophils.

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Induction of zymosan‐air‐pouch inflammation in rats and its characterization with reference to the effects of anticomplementary and anti‐inflammatory agents

Cited by 51 publications

References 32 publications

Intradermal air pouch leukocytosis as an in vivo test for nanoparticles

Intradermal air pouch leukocytosis as an in vivo test for nanoparticles

Analysis of the Factor(s) Involved in Pathogenesis of Zymosan-Induced Inflammation in Rats

The role of CD69 in acute neutrophil‐mediated inflammation

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