Induction of virus-neutralizing antibodies by immunization with Rachiplusia nu per os infected with a recombinant baculovirus expressing the E2 glycoprotein of bovine viral diarrhea virus

Expression and Immunological Studies of Classical Swine Fever Virus Glycoprotein E2 in the Bi-Cistronic Baculovirus/Larvae Expression System

“…By fluorescence intensity, the maximal yield of rE2 without degradation in the hemolymph at 72 hpi was about 600-800 mg/ml, much higher than the yield from other expression systems. 5,7,18) We found that mice immunized with rE2-containing hemolymph developed higher titers of anti-CSFV E2 antibodies than the negative controls after booster vaccinations, as reported by Ferrer et al 26) However, the most convincing evidence of the immunogenicity of CSFV E2 was obtained from the vaccination experiments with pigs. 27) Determination of the immunization efficacy of larva-derived rE2 in piglets was done in this study.…”

“…The vectors, viruses, cell lines, and insects used in this study are listed in Table 1. Sf9 cells were maintained at 26 C in TNM-FH (Trichoplusia ni modified formula Hank's) basal medium (Sigma, St. Louis, MO) supplemented with 10% heat-inactivated fetal bovine serum. T. ni larvae were reared in a non-sterile climatic chamber at 26 AE 1 C and fed an artificial insect diet, as described by O'Reilly et al…”

Potential Use of the Hemagglutinin-Neuraminidase Glycoprotein of Newcastle Disease Virus Expressed in Rachiplusia nu Larvae as an Immunogen for Chickens

“…Insect larvae infected with recombinant baculoviruses can be an extremely useful alternative due to the high levels of protein expression achieved, the lower costs for large-scale production, and a higher efficiency of certain types of posttranslational modifications (19). In the last 10 years, larvae have been successfully used to produce different recombinant proteins (3,5,7,13,16,21,22).…”

Complete Genome Sequence of a Bovine Viral Diarrhea Virus Strain Isolated in Southern China

“…The homologies at the nucleotide level are 89.0% to ~99.2%, and the homologies at the deduced amino acid level are 88.6% to ~97.2% for the BVDV-1b subtype. Immunogenicity studies have shown that the nonstructural 3 (NS3) and envelope 2 (E2) glycoproteins are crucial components for eliciting neutralizing antibodies in cattle (7, 8). However, the amino acid sequence of E2 of GX4, as with the other 29 BVDV-1b subtypes, is highly variable, whereas that of NS3 is conserved.…”