Induction of vasorelaxation through activation of nitric oxide synthase in endothelial cells by brazilin

Hu, Chien Ming; Kang, Jaw Jou; Lee, Chen Chen; Li, Ching Hao; Liao, Jiunn‐Wang; Cheng, Yu Wen

doi:10.1016/s0014-2999(03)01639-x

Cited by 64 publications

(45 citation statements)

References 48 publications

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“…Hu et al [20] previously reported that brazilin induced vasorelaxation only in intact but not denuded aorta, suggesting that the vasorelaxant effect of brazilin was depen- Acta Pharmacologica Sinica npg dent on endothelium, whereas in our study, different phenomena were observed. Brazilin dose-dependently relaxed NE and Ang II induced contraction in both intact and denuded aortic samples.…”

Section: Discussioncontrasting

confidence: 68%

“…In addition, the effects and some related mechanisms of brazilin in the vascular system have been described. Brazilin relaxed phenylephrine-induced vasoconstriction, and this response could be inhibited by [4,3-a]quinoxalin-1-one (ODQ) and hemoglobin, suggesting that the mechanism by which brazilin caused vasodilation might be endothelium-dependent [19,20] . However, vasoconstriction is a complicated process, involving not only endothelium but also other factors such as K + channels and Ca 2+ channels.…”

Section: Introductionmentioning

confidence: 99%

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Brazilin isolated from the heartwood of Caesalpinia sappan L induces endothelium-dependent and -independent relaxation of rat aortic rings

Yan¹,

Chen²,

Lin³

et al. 2015

Acta Pharmacol Sin

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Aim: Brazilin is one of the major constituents of Caesalpinia sappan L with various biological activities. This study sought to investigate the vasorelaxant effect of brazilin on isolated rat thoracic aorta and explore the underlying mechanisms. Methods: Endothelium-intact and -denuded aortic rings were prepared from rats. The tension of the preparations was recorded isometrically with a force displacement transducer connected to a polygraph. The phosphorylation levels of ERK1/2 and myosin light chain (MLC) were analyzed using Western blotting assay. Results: Application of brazilin (10-100 μmol/L) dose-dependently relaxed the NE-or high K + -induced sustained contraction of endothelium-intact aortic rings (the EC 50 was 83.51±5.6 and 79.79±4.57 μmol/L, respectively). The vasorelaxant effect of brazilin was significantly attenuated by endothelium removal or by pre-incubation with L-NAME, methylene blue or indomethacin. In addition, pre-incubation with brazilin dose-dependently attenuated the vasoconstriction induced by KCl, NE or Ang II. Pre-incubation with brazilin also markedly suppressed the high K + -induced extracellular Ca 2+ influx and NE-induced intracellular Ca 2+ release in endotheliumdenuded aortic rings. Pre-incubation with brazilin dose-dependently inhibited the NE-stimulated phosphorylation of ERK1/2 and MLC in both endothelium-intact and -denuded aortic rings. Conclusion: Brazilin induces relaxation in rat aortic rings via both endothelium-dependent and -independent ways as well as inhibiting NE-stimulated phosphorylation of ERK1/2 and MLC. Brazilin also attenuates vasoconstriction via blocking voltage-and receptoroperated Ca 2+ channels.

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Section: Discussioncontrasting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Brazilin isolated from the heartwood of Caesalpinia sappan L induces endothelium-dependent and -independent relaxation of rat aortic rings

Yan¹,

Chen²,

Lin³

et al. 2015

Acta Pharmacol Sin

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“…Brazilin (in SFN) has been reported to have many biological activities, including immune regulation, 19,20 blood vessel dilatation, 3,11,14 anticoagulation, 8,17 hypoglycemic effect, 10,13,15,16,26,27 oxidative damage inhibition, 6,28 antibiosis, 9 and anti-inflammatory effects. 4,5,7 In addition, it was demonstrated that brazilin powerfully broke deoxynucleic acid chains in anticancer screening.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

et al. 2015

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Background. Bladder cancer is a common malignant tumor with a very high recurrence rate after surgery. Intravesical instillation can help clear up the residual tumor cells after surgery and thereby reduce the recurrence rate. Objective. To establish a bladder tumor transplantation animal model and to evaluate the inhibitory effects of a novel perfusate, Su Fu'ning Lotion (SFN), on bladder tumor. Methods. SFN was compared with several commonly used chemotherapy drugs, including mitomycin (MMC) and pirarubicin (THP) for anticancer effects on the bladder cancer cell lines T24, BTT, and BIU-87 and SFN half inhibitory concentrations (IC 50 ) were determined after 48 hours of treatment. In addition, bladder cancer orthotopic transplantation tumor models were established in BALB/C nude mice and T739 mice, and SFN anticancer effects were assessed in vivo, with normal saline and MMC as negative and positive controls, respectively. Results. SFN, MMC, and THP were all lethal to bladder cancer cells, in vitro, with SFN and THP significantly superior to MMC. IC 50 values for SFN were 13.22, 11.22, and 12.5 µg/mL on T24, BTT, and BIU-87 cells, respectively. In vivo, SFN significantly reduced the mouse bladder wet weight and prolonged the animal survival compared with controls (P < .05), suggesting that SFN significantly inhibited T24/BTT cell growth in mice. Conclusion. SFN inhibited the bladder cancer cell proliferation in vitro and in vivo and significantly prolonged the survival of mice with bladder cancer xenografts, indicating that SFN could be used as a perfusate after surgery for removal of residual bladder cancers cells.

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“…This uncharged diatomic molecule is generated endogenously by three nitric oxide synthases (NOS) enzymes -endothelial NOS, neuronal NOS and inducible NOS -which catalyses the conversion of L-arginine to L-citrulline, yielding NO (Palmer, Ashton, Moncada, 1988;Ignarro, 1990;Zweier et al, 1995;Zhou, Zhu, 2009). Nitric oxide, a small molecule signaling agent (Fukuto et al, 2012), is associated with many biological processes (Toledo, Augusto, 2012) as vasodilatation (Furchgott, Zawadzki, 1980;Hu et al, 2003;Yetik-Anacak, Catravas, 2006;Pacher, Beckman, Liaudet, 2007), immune response (Bogdan, 2001; Bogdan, Röllinghoff, Diefenbach, 2000;…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide releasing-dendrimers: an overview

Roveda

Franco

2013

Braz. J. Pharm. Sci.

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Platforms able to storage, release or scavenge NO in a controlled and specific manner is interesting for biological applications. Among the possible matrices for these purposes, dendrimers are excellent candidates for that. These molecules have been used as drug delivery systems and exhibit interesting properties, like the possibility to perform chemical modifications on dendrimers surface, the capacity of storage high concentrations of compounds of interest in the same molecule and the ability to improve the solubility and the biocompatibility of the compounds bonded to it. This review emphasizes the recent progress in the development and in the biological applications of different NO-releasing dendrimers and the nitric oxide release pathways in these compounds.

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Induction of vasorelaxation through activation of nitric oxide synthase in endothelial cells by brazilin

Cited by 64 publications

References 48 publications

Brazilin isolated from the heartwood of Caesalpinia sappan L induces endothelium-dependent and -independent relaxation of rat aortic rings

Brazilin isolated from the heartwood of Caesalpinia sappan L induces endothelium-dependent and -independent relaxation of rat aortic rings

Evaluation of Su Fu’ning Lotion’s Inhibitory Effects on Bladder Cancer Cells In Vitro and In Vivo by Intravesical Instillation

Nitric oxide releasing-dendrimers: an overview

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