Induction of Vascular Endothelial Growth Factor Gene Expression by Interleukin-1 β in Rat Aortic Smooth Muscle Cells

Li, Jian; Perrella, Mark A.; Tsai, Jer‐Chia; Yet, Shaw‐Fang; Hsieh, Chung-Ming; Yoshizumi, Masao; Patterson, Cam; Endege, Wilson O.; Fang, Zhou; Lee, Mu-En

doi:10.1074/jbc.270.1.308

Cited by 307 publications

(166 citation statements)

References 33 publications

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“…These observations suggest that IL-1β may alter the functional properties of vascular cells, including the induction of angiogenesis. Vascular-biology studies have shown that IL-1β increase VEGF mRNA in rat aortic smooth muscle cells in a timeand dose-dependent manner (Li et al, 1995). This induction of VEGF expression was found to be due to an increase in transcription as well as an increase in the stability of its mRNA.…”

Section: Discussionmentioning

confidence: 94%

Regulation of vascular endothelial growth factor expression in human colon cancer by interleukin-1β

et al. 1999

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Summary Expression of vascular endothelial growth factor (VEGF), an important angiogenic factor in colon cancer, is tightly regulated by factors in the microenvironment. However, specific factors indigenous to the organ microenvironment of colon cancer growth that regulate VEGF expression in human colon cancer are not well defined. We investigated interleukin-1β (IL-1β) induction of VEGF expression in colon cancer cells and the mechanism by which this occurs. HT29 human colon cancer cells were treated with IL-1β for various periods. Induction of VEGF mRNA by IL-1β peaked at 24 h (> fivefold) and returned to baseline by 48 h. SW620 human colon cancer cells also reached a peak induction of VEGF mRNA 24 h after treatment with IL-1β. VEGF was induced at a dose range between 1 and 20 ng ml -1 of IL-1β. IL-1β induction of VEGF was also confirmed at the protein level. To examine the mechanism for VEGF induction by IL-1β, we transiently transfected VEGF promoter-reporter constructs into HT29 cells. IL-1β increased the activity of the VEGF promoter-reporter construct. Pretreatment of HT29 cells with dactinomycin abrogated the induction of VEGF mRNA by IL-1β. The half-life of VEGF mRNA was not prolonged by treatment with IL-1β. These findings suggest that IL-1β regulates VEGF expression in human colon cancer cells by increasing transcription of the VEGF gene.

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Section: Discussionmentioning

confidence: 94%

Regulation of vascular endothelial growth factor expression in human colon cancer by interleukin-1β

et al. 1999

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“…Although those results were obtained in another species (canine) and under different experimental conditions, it is apparent that the major angiogenic factor gene response for the skeletal and respiratory muscles is VEGF. The short half-life of VEGF mRNA levels in vascular smooth muscles [24] could explain the acute increase in VEGF as an early response to increased metabolic needs.…”

Section: Discussionmentioning

confidence: 99%

Diaphragmatic angiogenic growth factor mRNA responses to increased ventilation caused by hypoxia and hypercapnia

et al. 2001

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This study investigates the effect of increased ventilation on the expression of messenger ribonucleic acid (mRNA) levels of vascular endothelial growth factor (VEGF), basic ®broblast growth factor (bFGF) and transforming growth factorb 1 (TGF-b 1 ) in the diaphragm of intact, awake, spontaneously breathing rats, compared with responses in paralysed, mechanically-ventilated animals at similar blood gas and ventilatory levels.Four groups of intact, rats were studied in a body box, each group breathing one of four gases: room air, 12% oxygen (O 2 ), 5% carbon dioxide (CO 2 ), or 12% O 2 +5% CO 2 for 1 h. Another 4 groups of paralysed, mechanically-ventilated animals were matched for arterial blood gas and ventilatory level.The results showed that VEGF mRNA abundance was increased three-fold and that of bFGF 1.5-fold when 12% O 2 +5% CO 2 were breathed, but TGF-b 1 did not change. A signi®cant linear relationship of VEGF and bFGF mRNA to minute ventilation was observed in awake animals (r=0.98, p<0.02 and r=0.87, p<0.03, respectively). The paralysed, mechanically-ventilated animals showed no mRNA increases for any probe. Systemic hypoxia had no additional effect on VEGF or bFGF levels in the diaphragm.It was concluded that messenger ribonucleic acid for vascular endothelial growth factor and basic ®broblast growth factor in the diaphragm rises signi®cantly as a result of active ventilation and not due to blood gas/pH changes or to passive muscle shortening per se. Eur Respir J 2001; 17: 681±687.

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“…11) (Cohen et al, 1996;Goad et al, 1996;Hoffmann et al, 2002;Li et al, 1995;Pertovaara et al, 1994;Ryuto et al, 1996). The mechanisms by which these factors contribute to up-regulation of retinal VEGF transcription are not yet clear.…”

Section: Transcriptional Regulation Of Vegf Expression-tissuementioning

confidence: 99%

Vascular endothelial growth factor in eye disease

Penn

Madan

Caldwell

et al. 2008

Progress in Retinal and Eye Research

610

527

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Collectively, angiogenic ocular conditions represent the leading cause of irreversible vision loss in developed countries. In the U.S., for example, retinopathy of prematurity, diabetic retinopathy and age-related macular degeneration are the principal causes of blindness in the infant, working age and elderly populations, respectively. Evidence suggests that vascular endothelial growth factor (VEGF), a 40 kDa dimeric glycoprotein, promotes angiogenesis in each of these conditions, making it a highly significant therapeutic target. However, VEGF is pleiotropic, affecting a broad spectrum of endothelial, neuronal and glial behaviors, and confounding the validity of anti-VEGF strategies, particularly under chronic disease conditions. In fact, among other functions VEGF can influence cell proliferation, cell migration, proteolysis, cell survival and vessel permeability in a wide variety of biological contexts. This article will describe the roles played by VEGF in the pathogenesis of retinopathy of prematurity, diabetic retinopathy and age-related macular degeneration. The potential disadvantages of inhibiting VEGF will be discussed, as will the rationales for targeting other VEGF-related modulators of angiogenesis.

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Induction of Vascular Endothelial Growth Factor Gene Expression by Interleukin-1 β in Rat Aortic Smooth Muscle Cells

Cited by 307 publications

References 33 publications

Regulation of vascular endothelial growth factor expression in human colon cancer by interleukin-1β

Regulation of vascular endothelial growth factor expression in human colon cancer by interleukin-1β

Diaphragmatic angiogenic growth factor mRNA responses to increased ventilation caused by hypoxia and hypercapnia

Vascular endothelial growth factor in eye disease

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